Preparation method of novel skin filler

A dermal filler, a new type of technology, applied in medical science, prosthesis, etc., can solve the problems of complex post-processing, unsuitable for industrial scale-up, low grafting efficiency, etc., and achieve the effect of prolonging the residual time

Inactive Publication Date: 2016-06-29
SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Common methods for introducing amide bonds into sodium hyaluronate molecules include 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) activation, 2-chloro-1-methylpyridinium Iodide (CMPI) activation, etc., but most of these methods have many disadvantages, such as the EDC activation method has a strong dependence on the pH value and low grafting efficiency, which is not suitable for large-scale industrial scale-up; while the CMPI activation method There are disadvantages such as low reactivity and complicated post-processing

Method used

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  • Preparation method of novel skin filler
  • Preparation method of novel skin filler

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Dissolve 0.0914g of lysine in 15mL of water, adjust the pH to 4 with hydrochloric acid, weigh 1g of sodium hyaluronate dry powder, stir well, and put it at 4°C overnight. The next day, weigh 0.6918g of DMTMM and dissolve it completely with 5mL of water and add it to the gel. After stirring completely, react at 4°C for 3 days.

[0019] Cut the reacted gel into pieces, dialyze with PBS buffer, change the dialysate once every 1h and weigh it until the gel mass reaches 50g, then stop the dialysis, grind the gel into granules, and obtain cross-linked Sodium hyaluronate gel.

Embodiment 2

[0021] Dissolve 0.0914g of lysine in 15mL of water, adjust the pH to 6 with hydrochloric acid, weigh 1g of sodium hyaluronate dry powder, stir well, and put it at 4°C overnight. The next day, weigh 0.6918g of DMTMM and dissolve it completely with 5mL of water and add it to the gel. After stirring completely, react at 4°C for 3 days.

[0022] Cut the reacted gel into pieces, dialyze with PBS buffer, change the dialysate once every 1h and weigh it until the gel mass reaches 50g, then stop the dialysis, grind the gel into granules, and obtain cross-linked Sodium hyaluronate gel.

Embodiment 3

[0024] Dissolve 0.0914g of lysine in 15mL of water, adjust the pH to 6.5 with hydrochloric acid, weigh 1g of sodium hyaluronate dry powder, stir well, and put it at 4°C overnight. The next day, weigh 0.6918g of DMTMM and dissolve it completely with 5mL of water and add it to the gel. After stirring completely, react at 4°C for 3 days.

[0025] Cut the reacted gel into pieces, dialyze with PBS buffer, change the dialysate once every 1h and weigh it until the gel mass reaches 50g, then stop the dialysis, grind the gel into granules, and obtain cross-linked Sodium hyaluronate gel.

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Abstract

The present invention relates to a preparation method of a novel dermal filler. The method adopts low-toxic amino acid and its derivatives as cross-linking agent to prepare cross-linked sodium hyaluronate gel; Mild and easy to handle and other advantages, the basic amino acids-lysine and arginine or their derivatives among the essential amino acids for the human body are selected as the cross-linking agent to undergo a cross-linking reaction with the carboxyl end of the sodium hyaluronate molecule, and then partially seal the body The recognition site of hyaluronidase delays the recognition and degradation of the enzyme, and finally increases the residual time in the body. It provides a new technology for the development of safe and long-acting sodium hyaluronate injectable cosmetic fillers. Better market development prospects.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, and relates to a preparation method of a novel cross-linked sodium hyaluronate gel, which is used for medical filling and cosmetology. Background technique [0002] Sodium hyaluronate is an important component of human and animal skin, vitreous, joint lubricating fluid and cartilage tissue, which consists of (1-β-4) D -glucuronic acid and (1-beta-3) N -Acetyl- D -Glucosamine disaccharide units are repeatedly connected, and are widely used in repair surgery, eye surgery or as cosmetic products to fill wrinkles. Hyaluronic acid has good physical and chemical properties and biocompatibility. However, it is rapidly degraded by the enzymatic hydrolysis of hyaluronidase in the body, and its retention time is short, requiring repeated injections to achieve curative effect. Cross-linked hyaluronic acid is a polymer gel obtained by cross-linking and modifying hyaluronic acid with a cross-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075C08L5/08C08K5/357C08K5/17A61L27/20A61L27/60A61L27/52
Inventor 魏长征宋瑞瑞奚宏伟蒋丽霞
Owner SHANGHAI QISHENG BIOLOGICAL PREPARATION CO LTD
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