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Total bacterial count testing slip and preparation method and application thereof

A technology for the total number of colonies and test pieces, applied in biochemical equipment and methods, measurement/inspection of microorganisms, bioreactor/fermenter combination, etc., to achieve easy identification, good color rendering effect, and good color rendering effect

Active Publication Date: 2016-07-13
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these test strips have outstanding advantages of convenience and practicality, as a new technology for microbial detection, there are still some deficiencies in the test strips, and the technical bottlenecks in terms of bacterial growth, colony color diffusion, and accurate counting need to be effectively resolved.

Method used

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  • Total bacterial count testing slip and preparation method and application thereof
  • Total bacterial count testing slip and preparation method and application thereof
  • Total bacterial count testing slip and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 total bacterial colony assay culture medium

[0029] On the basis of the screening of the basic medium, further compare the effects of different growth-promoting factors, surfactants, loosening agents, and color-developing agents on the growth, color, and counting of colonies on the test piece, and optimize the content.

[0030] We determined the original weight components of the culture medium for color development of the total number of colonies: tryptone 20g, glucose 2.5g, yeast extract powder 5g, sodium chloride 5g, bean dregs 6g, mannitol 1g, sodium pyruvate 1g, Tween 801mL , TTC0.3g.

[0031] The preparation method of the culture medium for color development of the total number of colonies is:

[0032] Add 20g of tryptone, 5g of yeast extract powder, 5g of sodium chloride, 2.5g of glucose, and 6g of okara into 90mL of distilled water, heat and stir to dissolve, then add 1g of sodium pyruvate, 1g of mannitol, and 1mL of Tween 80 and...

Embodiment 2

[0035] The preparation of the total number of colony test piece of embodiment 2

[0036] 1) The test piece consists of three layers, namely the upper layer of PET silicone film, the middle layer of polypropylene plastic plate with hollowed out area, and the bottom plate of white square copper paper. The three parts are all cut into 11cm×9cm, the middle hollow area (7cm×7cm) is bonded with the bottom plate to form a culture area, and a light orange 1cm×1cm square is printed on the cardboard for easy counting. The upper, middle and lower parts are bonded to each other with polyacrylic resin pressure-sensitive adhesive, and the bonding width is 5mm.

[0037] 2) Apply the compound cold water soluble gel powder evenly on the inner side of the upper film, with a thickness of 0.15mm, and the mass of cold water gel for each test piece is 0.2g; put the above test pieces into a sterilizer at 121°C under high pressure After sterilizing for 15 minutes, cool to room temperature.

[0038]...

Embodiment 3

[0041] Embodiment 3 Utilizes test sheet to measure the method for the total number of bacterial colonies in food

[0042] 1) Weigh 25g of the food sample to be inspected (the sample to be inspected is solid food such as cooked meat products and biscuits) by aseptic operation, or add 25mL of the food sample to be inspected (the sample to be inspected is liquid food such as beverage) to 225mL of normal saline In a sterile Erlenmeyer flask, mix thoroughly to make a 1:10 homogeneous sample solution.

[0043] 2) Use a 1mL micropipette to draw 1mL of the 1:10 sample homogeneous solution, slowly pour it along the tube wall into a sterile test tube containing 9mL of physiological saline diluent, mix well, and make a 1:100 sample homogeneous solution. Further 10-fold serial dilutions were prepared to obtain serial dilutions of 1:1000, 1:10000, and 1:100000. spare.

[0044] 3) Take 1mL of the dilution solution of the food sample to be tested (the five dilution solutions obtained in st...

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Abstract

The invention discloses a total bacterial count testing slip and a preparation method and application thereof in the aspect of detecting the total bacterial count in food, and belongs to the technical field of food detection.The testing slip is composed of a transparent plastic upper-layer film with the sticky inner side, a plastic middle-layer plate with a hollow area and a bottom plate with printed squares.The inner side of the upper-layer film is coated with a composite cold water soluble gel, and the hollow area of the middle-layer plate is filled with a total bacterial count detection culture medium.After total bacterial count testing is carried out, a bacterial clump is red on the testing slip, and the test slip with the moderate bacterial count is selected for counting.The total bacterial count testing slip has the advantages that after sample liquid to be detected is added into the testing slip, a composite cold water gel and culture medium components form a compact net gel, a growth promoter in the culture medium has the effects of promoting and repairing bacteria growth, and a color developing agent, a bulking agent and a surfactant are beneficial to even distribution of sample liquid, bacterial clump forming and visual counting.When the total bacterial count testing slip is used for testing the total bacterial count in food, the program is easy and convenient to implement, the bacterial clump has the clear color, and counting is accurate.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a test piece for the total number of bacterial colonies, a preparation method and its application in detecting the total number of bacterial colonies in food. Background technique [0002] The total number of bacterial colonies is an important indicator reflecting the hygienic quality of food, and it is used to determine the degree of food contamination by bacteria. The determination of the total number of colonies is a detection index in the detection standards of various agricultural products and foods such as cooked meat products, aquatic products, grain products, instant fruit and vegetable products, instant condiments, beverages, etc., and is also an important monitoring item for food risk prediction and hazard assessment. At present, GB4789.2 "National Food Safety Standard for Determination of Total Bacterial Colony in Food Microbiological Examination" ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34
CPCC12Q1/06
Inventor 陈萍王艳蕊王佳男李玉扩陈红王秀娟任大勇毕云枫
Owner JILIN AGRICULTURAL UNIV
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