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Grifolan extracting method and pharmaceutical application thereof

A polysaccharide and extraction method of Grifola frondosa is applied in the directions of medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients to achieve the effects of improving extraction efficiency, promoting proliferation, and promoting healing and repairing.

Inactive Publication Date: 2016-07-20
ZHANGZHOU PIEN TZE HUANG PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of pharmacological studies have proved that Grifola frondosa polysaccharide (GFP) has significant anti-tumor, hypoglycemic, hypolipidemic, inhibiting hepatitis B DNA replication, anti-HIV virus, anti-oxidation and improving immune system functions. Reports on its application in repairing gastric mucosal trauma

Method used

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  • Grifolan extracting method and pharmaceutical application thereof
  • Grifolan extracting method and pharmaceutical application thereof
  • Grifolan extracting method and pharmaceutical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: Extraction of Grifola frondosa polysaccharide

[0032] Take the dry fruiting body of Grifola frondosa, pass through a 10-mesh sieve after crushing to obtain the fruiting body particles of Grifola frondosa, add 10 times the amount (mass volume ratio) of water and boil for 2 hours; filter, and use 8 times the amount of 2% NaOH for the filter residue Extract the solution for 2 hours; filter, take the filter residue and add 8 times the amount of 1% acetic acid solution to extract for 2 hours; filter, concentrate the filtrate to Brix (soluble solids) ≥ 15%, add 95% ethanol according to the volume ratio of 1:3 Carry out alcohol precipitation for 4 hours, filter, redissolve the filter residue in water, and centrifuge at 10,000 rpm for 10 minutes, and the precipitate obtained is the extract, which is Grifola frondosa polysaccharide with a polysaccharide content of 19%.

Embodiment 2

[0033] Example 2: Effects of Grifola frondosa polysaccharides on the proliferation of human gastric mucosal epithelial cells

[0034] 1. Materials

[0035] Cell line: human normal gastric mucosal epithelial cells GES-1 were purchased from Beijing Institute of Cancer Prevention and Control.

[0036] Medium: The basal medium for GES-1 cell culture is DMEM medium (10% FBS, 100U penicillin, 100U streptomycin).

[0037] Grifola frondosa polysaccharide GFP (polysaccharide of Grifola frondosa): the Grifola frondosa polysaccharide prepared in Example 1 was used.

[0038] 2. Method

[0039] The effect of GFP on the proliferation activity of GES-1 was detected by MTS method. GES-1 in the logarithmic growth phase was digested with trypsin and its concentration was adjusted to 2.0×10 4 cells / mL, seeded in a 96-well plate, 100 μL per well, placed at 37°C, saturated humidity, 5% CO 2 After 24 hours of culture in a sterile incubator, the old culture medium was discarded. The experimenta...

Embodiment 3

[0045] Example 3: Effects of Grifola frondosa polysaccharides on human gastric mucosal wound healing and repair

[0046] 1. Material: Same as Example 2

[0047] 2. Method

[0048] Select GES-1 in the logarithmic growth phase, and use 6×10 per well 4 Cells were seeded in 24-well plates, 400 μL per well. Set at 37°C, 5% CO 2 Cultivate in the incubator for 24 hours. After the cells form a relatively tight connection to form a single layer, use a capillary tube with a diameter of 1.5 mm to draw a vertical trace in the center of each well of the 24-well plate, and the two sides of the wound can be seen under the microscope at the same time. side. Aspirate the original culture medium after scratching, wash each well twice with 1mL PBS to remove non-adherent cells, select the final concentration of GFP as 2, 0.4, 0.08mg / mL according to the results of the proliferation experiment, and culture medium 400μL, the control group only added 400 μL of DMEM basal medium. For each concen...

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Abstract

The invention discloses a grifolan extracting method and the pharmaceutical application thereof.Grifolan is obtained through aqueous extraction, alkaline extraction, acid extraction and alcohol precipitation.It is found that by improving the expression level of mRNA and protein of TFF2 and the expression level of protein of EGF and reducing the expression level of mRNA and protein of TGF-beta 1, proliferation of gastric mucosa epithelial cells and migration of gastric mucosa epithelial cells to a gastric mucosa wound area can be promoted, and then gastric mucosa healing and repairing are promoted and a basis is provided for development and utilization of grifola frondosa and treatment of gastric mucosa wounds and chronic gastritis.

Description

technical field [0001] The invention relates to a method for extracting polysaccharide from Grifola frondosa and its pharmaceutical application. Background technique [0002] The gastric mucosa has a tissue structure that prevents gastric juice from digesting itself and the stimulation of various damage factors, and protects the physiological functions of gastric mucosal cells. The integrity of this barrier is an important basis for the protection of gastric mucosa. Gastric mucosa is often caused by chemical factors (drinking, strong tea, coffee, smoking and irritating drugs such as aspirin, indomethacin, etc.), physical factors (too cold, too hot, too rough food or overeating), bacteria or their toxins. The cause of the irritation caused the injury. The clinical manifestations are a series of symptoms such as pain and discomfort in the epigastric cavity, fullness, belching, acid reflux, stomach burning, nausea, and vomiting. Gastric mucosal changes of different degrees of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61K31/715A61P1/04
CPCC08B37/0024A61K31/715C08B37/0003
Inventor 潘裕添王丽菊黄家福洪绯
Owner ZHANGZHOU PIEN TZE HUANG PHARM
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