A method for extracting protodioscin from yam plants
A technology of protodioscin and plants, which is applied in the field of extracting high-purity protodioscin, which can solve the problems of hydrolysis interference, large water consumption, and large waste water discharge, so as to improve purity and yield, reduce residues, and improve efficiency Effect
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Embodiment 1
[0027] The raw material of this embodiment adopts Dioscorea chinensis. A method for extracting protodioscin from yam plants, comprising the following steps: crushing the raw material yam yam to 15 mesh, adding water three times the weight of the dry weight of the raw material and a compound enzyme of 1.5% of the dry weight of the raw material to carry out enzymatic hydrolysis for 120 minutes; The compound enzyme is composed of cellulase and amylase in a weight ratio of 3:1; after enzymolysis, filter, take the filter residue to granulate, and dry at 50°C for 2.5h after granulation; then perform supercritical extraction to keep CO 2 The flow rate is 25l / h, the pressure is 20MPa, and the extraction temperature is 55°C; after extraction for 1.5h, it is put into a separation tank for separation, the separation pressure is 15MPa, and the separation temperature is 45°C, and the protodioscin product is obtained by separation. After testing, the obtained protodioscin has a purity of 95...
Embodiment 2
[0029] The raw material of this embodiment adopts Dioscorea scutellariae. A method for extracting protodioscin from yam plants, comprising the following steps: crushing the raw material yam dioscens to 20 mesh, adding water twice the weight of the dry weight of the raw material and a compound enzyme of 1% of the dry weight of the raw material to carry out enzymatic hydrolysis for 90 minutes; The compound enzyme is composed of cellulase and amylase in a weight ratio of 3:1; after enzymolysis, filter, take the filter residue to granulate, and dry at 40°C for 2 hours after granulation; then perform supercritical extraction to keep CO 2 The flow rate is 15l / h, the pressure is 15MPa, and the extraction temperature is 50°C; after extraction for 1h, it is put into a separation tank for separation, the separation pressure is 10MPa, and the separation temperature is 35°C, and the protodioscin product is obtained by separation. After testing, the obtained protodioscin has a purity of 98...
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