Method for ultrasonic alkali treatment-based dual-enzyme degradation of bagasse

A technology of ultrasonic and alkali treatment, applied in the field of bagasse resource utilization, can solve the problem that the effect is not obvious, and achieve the effects of low cost, less by-products, and improved degradation rate

Inactive Publication Date: 2016-08-10
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, the yield of cellulose is at most 54%, and the structure of cellulose is relatively dense, so that the effect of enzymolysis will not be obvious

Method used

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  • Method for ultrasonic alkali treatment-based dual-enzyme degradation of bagasse
  • Method for ultrasonic alkali treatment-based dual-enzyme degradation of bagasse
  • Method for ultrasonic alkali treatment-based dual-enzyme degradation of bagasse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Place the bagasse in a dry and ventilated condition to air-dry, crush the air-dried bagasse with a mechanical mixer and sieve (60 mesh), put the sieved bagasse in an oven at 105°C to dry to remove its moisture to constant Re-obtain bagasse powder; in the bagasse powder after drying, pour NaOH solution (mass concentration is 2%) by the solid-to-liquid mass volume ratio 1: 12 of bagasse powder and NaOH solution, and carry out pretreatment with ultrasonic cleaner (power 200W, time 40min); use 10-20ml of sodium citrate buffer to adjust the pH of the system to 4 and add cellulase (0.05g / g bagasse) and xylanase (0.1g / g bagasse) for enzymolysis reaction for 32h (The reaction temperature is 50° C.), and the enzymolysis solution after enzymolysis was treated at 95° C. for 3 hours to inactivate the enzyme. Afterwards, centrifuge with a centrifuge (rotating speed 3000 rap, time 15 min), take the supernatant and use the DNS method to measure the reducing sugar content.

[0027] Re...

Embodiment 2

[0029] Place the bagasse in a dry and ventilated condition to air-dry, crush the air-dried bagasse with a mechanical mixer and sieve (60 mesh), put the sieved bagasse in an oven at 105°C to dry to remove its moisture to constant Re-obtain bagasse powder; in the bagasse powder after drying, pour NaOH solution (mass concentration is 3%) by the solid-to-liquid mass volume ratio 1: 13 of bagasse powder and NaOH solution, and carry out pretreatment with ultrasonic cleaner (power 300W, time 50min); use 10-20ml of sodium citrate buffer to adjust the pH of the system to 5 and add cellulase (0.07g / g bagasse) and xylanase (0.2g / g bagasse) for enzymolysis reaction for 32h (The reaction temperature is 50° C.), and the enzymolysis solution after enzymolysis was treated at 95° C. for 3 hours to inactivate the enzyme. Afterwards, centrifuge with a centrifuge (rotating speed 4000 rap, time 15 min), take the supernatant and use the DNS method to measure the reducing sugar content.

[0030] Re...

Embodiment 3

[0032] Place the bagasse in a dry and ventilated condition to air-dry, crush the air-dried bagasse with a mechanical mixer and sieve (80 mesh), put the sieved bagasse in an oven at 105°C to dry to remove its moisture to constant Re-obtain bagasse powder; in the bagasse powder after drying, pour NaOH solution (mass concentration is 5%) by the solid-to-liquid mass volume ratio 1: 14 of bagasse powder and NaOH solution, and carry out pretreatment with ultrasonic cleaner (power 300W, time 60min); use 10-20ml of sodium citrate buffer to adjust the pH of the system to 6 and add cellulase (0.08g / g bagasse) and xylanase (0.2g / g bagasse) for enzymolysis reaction for 35h (The reaction temperature is 50° C.), and the enzymolysis solution after enzymolysis was treated at 95° C. for 3 hours to inactivate the enzyme. Afterwards, centrifuge with a centrifuge (rotating speed 3000 rap, time 15 min), take the supernatant and use the DNS method to measure the reducing sugar content.

[0033] Re...

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Abstract

The invention discloses a method for ultrasonic alkali treatment-based dual-enzyme degradation of bagasse. The method utilizes bagasse as a raw material. Through supersonic wave-alkali pre-treatment and then cellulase-xylanase synergism-based enzymatic hydrolysis reaction, reducing sugar is produced. The method utilizes supersonic wave cavitation and mechanical action and alkali swelling and saponification effects on lignocelluloses to loose a lignocellulose structure and increase enzyme action sites and to remove lignin components for inhibiting cellulose hydrolysis so that a follow-up hydrolysis process is promoted and action time is shortened. Through synergism of cellulase and xylanase, a complex lignocelluloses space effect is simplified, more enzyme binding sites are provided, lignin content is reduced, hydrolysis time is shortened, enzymatic hydrolysis efficiency is improved, energy consumption is reduced and a reducing sugar yield is improved. The method solves the traditional process defects and has the advantages of high recycling rate, mild reaction conditions, full bagasse degradation, low cost, less side products and high reducing sugar yield.

Description

technical field [0001] The invention belongs to the technical field of bagasse resource utilization, and in particular relates to a method for degrading bagasse by ultrasonic alkali treatment and double enzymes. Background technique [0002] At present, the relatively mature process of producing fuel ethanol is to adopt the method of bioconversion, using fuel ethanol as fuel instead of fossil energy, which can effectively reduce the emission of harmful substances in the atmosphere. On the other hand, sugar refining, as a pillar industry in Guangxi, produces about 16 million tons of bagasse every year, and most of the bagasse is used for boiler combustion, resulting in a waste of biomass energy. If bagasse can be used as raw material for comprehensive utilization, the waste of bagasse can be reduced and resource utilization can be realized. However, how to optimize the utilization of lignocellulose in production is still a problem to be solved. [0003] Lignocellulose has a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02C12P19/00C12P19/12
CPCC12P19/14C12P19/00C12P19/02C12P19/12C12P2201/00
Inventor 孙卫东唐湘毅戴莉田雯苏芬芬
Owner GUANGXI UNIV
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