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Preparation method of electro-chemiluminescence immunosensor based on silver-hybridized bismuth sulfide

A technology of luminescence immunity and electrochemistry, applied in chemiluminescence/bioluminescence, scientific instruments, and analysis by chemical reaction of materials, etc., can solve the problems of long detection period, radioactive contamination, low sensitivity, etc. Low, sensitive specificity detection, the effect of enhanced sensitivity

Active Publication Date: 2016-08-10
上海佑科仪器仪表有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method has the disadvantages of radioactive pollution, low sensitivity, long detection cycle, cumbersome steps, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 A preparation method of electrochemiluminescent sensor for prostate-specific antigen based on gold-hybridized aminated graphene / ceria

[0039] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;

[0040] (2) Put 6 μL of the base material gold-hybridized amidated graphene / ceria solution at a concentration of 1 mg / mL on the surface of the electrode and store it at 4 °C;

[0041] (3) Add 6 µL, 5 µg / mL antibody dropwise, rinse the electrode surface with ultrapure water after drying, and dry in a refrigerator at 4 °C;

[0042] (4) Block non-specific active sites with 3 μL bovine serum albumin solution with a mass fraction of 1%, incubate in a refrigerator at 4 °C for 3 h, and clean;

[0043] (5) Add 6 μL of prostate-specific antigen dropwise on the electrode to capture the antibody, incubate at room temperature for 1 h, and wash it;

[0044] (6) ...

Embodiment 2

[0045] Example 2 A preparation method of electrochemiluminescent sensor for prostate-specific antigen based on gold-hybridized aminated graphene / ceria

[0046] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;

[0047] (2) Put 6 μL of the base material gold hybrid aminated graphene / ceria solution with a concentration of 2 mg / mL on the surface of the electrode and store it at 4 °C;

[0048] (3) Add 6 µL, 8 µg / mL antibody dropwise, rinse the surface of the electrode with ultrapure water after drying, and dry it in a refrigerator at 4 °C;

[0049] (4) Block non-specific active sites with 3 μL bovine serum albumin solution with a mass fraction of 1%, incubate in a refrigerator at 4 °C for 3 h, and clean;

[0050] (5) Add 6 μL prostate-specific antigen dropwise on the electrode to capture the specificity of the antibody, incubate at room temperature for 1 h, a...

Embodiment 3

[0052] Example 3 A preparation method of electrochemiluminescent sensor for prostate-specific antigen based on gold-hybridized aminated graphene / ceria

[0053] (1) Polish the glassy carbon electrode with a diameter of 4 mm with 1.0 μm, 0.3 μm, and 0.05 μm alumina polishing powder in sequence, and rinse it with ultrapure water;

[0054] (2) Put 6 μL of the base material gold-hybridized amidated graphene / ceria solution with a concentration of 3 mg / mL on the surface of the electrode and store it at 4 °C;

[0055] (3) Add 6 µL, 10 µg / mL antibody dropwise, rinse the surface of the electrode with ultrapure water after drying, and dry it in a refrigerator at 4 °C;

[0056] (4) Block non-specific active sites with 3 μL bovine serum albumin solution with a mass fraction of 1%, incubate in a refrigerator at 4 °C for 3 h, and clean;

[0057] (5) Add 6 μL of prostate-specific antigen dropwise on the electrode to capture the antibody, incubate at room temperature for 1 h, and wash it;...

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Abstract

The invention relates to a preparation method of an electro-chemiluminescence immunosensor based on silver-hybridized bismuth sulfide and belongs to the field of electro-chemiluminescence sensors. With cerium dioxide as an electro-chemiluminescence signal source, supporting quantity of an antibody can be increased by means of excellent biocompatibility and large specific surface area of gold-hybridized aminated graphene. With the silver-hybridized bismuth sulfide as a second antibody marker, detection on a prostate specific antigen is achieved according to different electro-chemiluminescence signal intensity of to-be-detected substance in different concentrations.

Description

technical field [0001] The invention relates to a preparation method of an electrochemiluminescence immunosensor based on silver hybrid bismuth sulfide. It specifically relates to a gold hybrid aminated graphene / cerium oxide as a base material, using the biocompatibility of gold nanoparticles and the large specific surface area of ​​aminated graphene to immobilize cerium dioxide and detect antibodies, and prepare a An electrochemiluminescent immunosensor for detecting prostate specific antigen belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] Prostate Specific Antigen (PSA) is a single-chain glycoprotein secreted by the epithelial cells of the prostate acini and ducts. It is functionally a serine protease similar to kallikrein and participates in the liquefaction process of semen. It is an important indicator that is routinely used in the diagnosis and differential diagnosis of benign and malignant prostate di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/574G01N33/543G01N21/76
CPCG01N21/76G01N33/54366G01N33/57434G01N33/58G01N33/6893G01N2800/342
Inventor 赵永蓓魏琴杜斌马洪敏张勇吴丹孙旭范大伟庞雪辉王欢
Owner 上海佑科仪器仪表有限公司
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