Purpose of Lactobacillus reuteri GMNL-263 for preparing hypolipidemic composition

A technology of Lactobacillus reuteri and a composition is applied in the field of use for preparing blood lipid-lowering compositions, and can solve problems such as probiotics for lowering blood lipids that are rarely discussed

Active Publication Date: 2016-08-17
GENMONT BIOTECH
View PDF6 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, according to the above studies, the application of probiotics in

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Purpose of Lactobacillus reuteri GMNL-263 for preparing hypolipidemic composition
  • Purpose of Lactobacillus reuteri GMNL-263 for preparing hypolipidemic composition
  • Purpose of Lactobacillus reuteri GMNL-263 for preparing hypolipidemic composition

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Preparation of GMNL-263 heat-killing bacteria: Lactobacillus reuteri GMNL-263 is stored in Jingyue Biotechnology Co., Ltd. strain depository. The standard strain Lactobacillus reuteri GMNL-263 stored in a frozen vial was inoculated into MRS medium, and cultured in an incubator at 37±1°C for 18 to 36 hours. After condensing the bacteria in the fermented bacteria liquid, use high temperature to heat to kill the bacteria. Finally, the protective agent is added and freeze-dried to make Lactobacillus reuteri GMNL-2632×10 per gram 10 Cells heat kills bacteria powder.

[0024] In addition, the following specific examples can further prove the scope of practical application of the present invention, but it is not intended to limit the scope of the present invention in any form.

[0025] First, 6-week-old weaned male hamsters of the malet strain were purchased from the National Laboratory Animal Center of Taiwan (Taipei, Taiwan), and were raised in plastic cages, 8 per group, 32 in ...

Example Embodiment

[0035] Example 1: Analysis of the biochemical content of serum, liver and feces

[0036] Serum biochemical value analysis: The test items are the lipid concentration in the blood, including triglycerides (TG), total cholesterol (T-CHO), high-density lipoprotein cholesterol (HDL-CHO) and low-density lipoprotein cholesterol (LDL) -CHO), the above test items are all commissioned to analyze by the National Laboratory Animal Center of Taiwan, China.

[0037] Analysis of malonaldehyde (MDA) content in blood: Use the commercially available TBARS Assay Kit (Cayman) to determine the content of malonaldehyde in the serum. Refer to the manufacturer’s instructions for the experimental procedures, and finally analyze by disk spectroscopy at a wavelength of 530nm The instrument measures the absorbance, and calculates the cytokine content in the sample with the standard concentration curve.

[0038] Lipid analysis of liver and feces: Take 0.1g of the analyzed tissue and homogenize it in 1ml of chl...

Example Embodiment

[0045] Example 2: Analysis of the effect of GMNL-263 heat-killing bacteria on mRNA gene expression in liver and adipose tissue

[0046] Use RT-PCR to detect (I) liver tissue and (II) adipose tissue mRNA gene expression. The detected genes are:

[0047] 1. Inflammation-promoting factors: interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α), the expression of the above genes can cause inflammation.

[0048] 2. Lipid synthesis related genes: fatty acid synthase (FAS), cholesterol regulatory element binding protein (sterol response element binding protein-1, SREBP-1c), peroxisome proliferator activated receptor γ ( peroxisome prolifera proliferator-activated receptor gamma (PPAR gamma); among them, fatty acid synthase (FAS) and cholesterol regulatory element binding protein (SREBP-1c) genes are involved in the synthesis of fatty acids, and peroxisome proliferator activated receptor gamma (PPAR gamma) It is related to the metabolism of fatty acids.

[0049] 3. Genes related to cholest...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a purpose of Lactobacillus reuteri GMNL-263 for preparing a hypolipidemic composition. A preservation number of the Lactobacillus reuteri GMNL-263 is BCRC 910452 and CCTCC M 209263, and the Lactobacillus reuteri can specifically inhibit the expression of related genes for synthesis of inflammation factors and lipid as well as can increase the expression of cholesterol metabolism-related genes; and thereby, the Lactobacillus reuteri GMNL-263 can be used for preparing the hypolipidemic composition, and reaches the purpose of treatment of hyperlipidemia.

Description

technical field [0001] The present invention relates to the use of Lactobacillus reuteri GMNL-263 for the preparation of blood lipid-lowering composition, especially refers to the composition containing Lactobacillus reuteri which has the ability to inhibit tumor necrosis factor α (TNF-α), fatty acid synthesis Enzyme (FAS), cholesterol regulatory element binding protein (SREBP-1c) gene expression, and enhance the ability of low-density lipoprotein receptor (LDLR) and cholesterol 7α-hydroxylase (CYP7A1) gene expression, to achieve the purpose of reducing blood lipids. Background technique [0002] Due to factors such as the increased chance of eating out and the refined diet of modern people, modern people consume too many calories and accumulate fat in the body, so they are prone to hyperlipidemia. According to research, hyperlipidemia not only easily leads to fatty liver or atherosclerosis, but also is an important risk factor for hypertension, heart disease, stroke, diabet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K35/747A61P3/06A23L1/30
Inventor 陈奕兴陈雅惠楼子齐沈庭鋆
Owner GENMONT BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap