Molecularly imprinted polymer for detecting ofloxacin and preparing method and application thereof

A molecular imprinting, polymer technology, applied in chemical instruments and methods, other chemical processes, material electrochemical variables, etc., can solve problems such as affecting the ecological environment, affecting microbial functions, and improving bacterial resistance, achieving strong selectivity. , Detection of wide linear range, less susceptible to interference

Active Publication Date: 2016-08-17
JIAXING UNIV
View PDF3 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of ofloxacin will increase bacterial resistance, affect micr

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecularly imprinted polymer for detecting ofloxacin and preparing method and application thereof
  • Molecularly imprinted polymer for detecting ofloxacin and preparing method and application thereof
  • Molecularly imprinted polymer for detecting ofloxacin and preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 graphene / rhodium nanoparticle preparation

[0040] Dissolve 20mg of graphene oxide (GO) in 20mL of ethanol, and disperse it evenly by ultrasonication for 1h; then add 400μL of 0.1M RhCl 3 solution and 4.0 x 10 -4 mol of sodium borohydride, ultrasonication for two hours; finally, the solution was condensed and refluxed at 90°C for 12 hours to obtain a black suspension solution, which was washed by centrifugation and dried overnight in a vacuum oven to obtain graphene / rhodium nanoparticles (GN / Rh).

Embodiment 2

[0041] Embodiment 2 ionic liquid preparation

[0042] (1) 3,3'-(1',4'-butane)-bis-1-vinylimidazolium bromide ([C 4 (mim) 2 ][Br 2 ])Synthesis

[0043] Mix 0.1mol 1-vinylimidazole and 0.05mol 1,4-dibromobutane evenly, add 30mL methanol, stir the mixture at 60°C for 15h to obtain a light yellow viscous liquid, cool to room temperature after the reaction is complete . Then the viscous solution was poured into 500mL anhydrous ether for washing, and the lower layer of viscous solution was decanted gently and vacuum-dried overnight at room temperature to constant weight to obtain [C 4 (mim) 2 ][Br 2 ]. NMR characterization obtained [C 4 (mim) 2 ][Br 2 ], the result is as follows: 1HNMR (400MHz, DMSO-d 6 ,δ,ppm):9.54s,2H),8.28(s,2H),8.02(s,2H),7.40dd,2H),6.00(d,2H),5.41(d,2H),4.29(s, 4H), 1.87(s, 4H); 13C NMR ((400MHz, DMSO-d 6 , δ, ppm): 135.66, 129.28, 123.71, 119.19, 109.06, 48.39, 25.75.

[0044] The specific reaction formula is:

[0045]

[0046] (2) 3,3′-(1′,4...

Embodiment 3

[0050] Example 3 Preparation of Molecularly Imprinted Polymers of the Present Invention

[0051] First, 0.080g carrier GN / Rh, 0.1mmol template molecule ofloxacin and 0.4mmol functional monomer ferrocenylmethyl methacrylate (VFM) were dispersed in 30mL of acetonitrile and toluene mixed solution (V: V=1:1), after ultrasonic mixing, 2.0mmol cross-linking agent [C 4 (mim) 2 ][(PF 6 ) 2 ] and 20mg of initiator azobisisobutylcyanide were added to the mixed solution, and nitrogen gas was introduced for 15 minutes, and then the reaction vessel was sealed. Then, it was reacted in an oil bath at 60°C for 24h, and the obtained solid product was dried in a vacuum oven at 50°C. Finally, Soxhlet extraction was carried out with a Soxhlet extractor, and the mixed solution of methanol and acetic acid (V:V=9:1) eluted the template molecules to obtain the molecularly imprinted polymer GN / Rh-MIP. At the same time, the molecularly imprinted polymer (GN / Rh-MIPMAA) with methacrylic acid as the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a molecularly imprinted polymer for detecting ofloxacin and a preparing method and application thereof. A carrier, a template molecule, a functional monomer and a crosslinking agent are polymerized under the action of an initiating agent to form a composite material, and the template molecule is removed from the composite material to obtain the molecularly imprinted polymer; graphene/rhodium nano particles serve as the carrier, the template molecule is ofloxacin, the functional monomer is methyl ferrocenyl methacrylate, and the crosslinking agent is 3,3'-(1',4'-butane)-bi-1-vinyl imidazole hexafluorophosphoric acid. The invention further discloses application of an electrochemical transducer made of the molecularly imprinted polymer to ofloxacin detection. The electrochemical transducer made of the molecularly imprinted polymer has good adsorptive property to ofloxacin and is high in selectivity, not prone to interference and wide in detection linear range, and the lower detection limit can reach 0.06 micrometer.

Description

technical field [0001] The invention relates to a molecularly imprinted electrochemical sensor, in particular to a molecularly imprinted polymer used for detecting ofloxacin and its preparation method and application. Background technique [0002] Since the discovery of penicillin by Alexander Fleming in 1928, antibiotics have developed rapidly. In the 1840s, antibiotics gradually began to be used in human and veterinary medicine to treat most diseases caused by bacterial and microbial infections. Studies have shown that about 20% of the antibiotics entering the human body are used to treat diseases, and 70% to 90% are excreted with the human body. Moreover, the sewage discharged from pharmaceutical factories and the medical wastewater from hospitals contain a large amount of antibiotics. These antibiotics are adsorbed on The sediment of the water body enters the domestic sewage, thereby affecting the ecological environment. [0003] Ofloxacin (OF), also known as ofloxacin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08F292/00C08F226/06C08F220/14C08J9/26B01J20/26B01J20/30G01N27/26
CPCB01J20/268B01J20/3071C08F292/00C08J9/26C08J2351/10G01N27/26C08F226/06
Inventor 李蕾曾延波唐欢张剑朱旭东徐金雷张晏
Owner JIAXING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap