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Method for preparing pumpkin seed polypeptide-containing cosmetics

A pumpkin seed and mass fraction technology, which is applied in the field of cosmetics containing pumpkin seed polypeptide and its preparation, can solve the problems of pumpkin seed polypeptide not being added and not involved in application fields, and achieve good water solubility, wide application prospects, strong resistance Oxidative effect

Inactive Publication Date: 2016-08-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are invention patents disclosing the preparation methods of pumpkin seed antioxidant peptides and low-molecular-weight peptides, all of which are limited to the preparation methods, but do not involve their specific application fields, and pumpkin seed peptides have not been added to cosmetics

Method used

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  • Method for preparing pumpkin seed polypeptide-containing cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1 Pumpkin Seed Crude Polypeptide and Preparation of Purified Polypeptide

[0013] Crush the pumpkin seed meal, remove the remaining oil with n-hexane, crush and sieve. Use alkali extraction and acid precipitation method, add deionized water according to the ratio of material to liquid 1:30g / mL, adjust the pH to 9.5, stir at 45°C for 2 hours, adjust the pH of the centrifuged supernatant to the isoelectric point of protein 3.4, and centrifuge to collect the precipitate. The precipitate was washed 4 times with water, and heated for 30 min to fully denature the protein. The centrifuged precipitate was freeze-dried to obtain powdered pumpkin seed protein.

[0014] The pumpkin seed protein is added with 2-3% (g / g) alkaline protease according to the substrate concentration of 5%, and the pH is controlled at 9.5 at 55° C., and hydrolyzed for 3 hours. Heat to 95°C to inactivate the enzyme for 20 minutes, centrifuge to take the supernatant and freeze-dry to obtain po...

Embodiment 2

[0019] Embodiment 2 Tightening and revitalizing night cream, based on 1000g of the total amount.

[0020] Water phase: glycerin 50g, deionized water 460.5g, sodium hydroxide 0.5g.

[0021] Oil Phase: Sorbitan Trioleate 20g, Sorbitan Tristearate 15g, Cetearyl Alcohol 50g, Squalane 100g, Caprylic / Capric Triglyceride 40g, Jojoba Oil 20g, Glyceryl monostearate 30g, polydimethylsiloxane 80g, retinol 10g, octyldodecanol 60g, phenoxyethanol 5g.

[0022] Post-addition phase: 15g of acrylic acid (ester) copolymer, 10g of panthenol, 30g of purified polypeptide from pumpkin seeds, 2g of caprylyl glycol, and 2g of essence.

[0023] Production process: Add the raw materials of the oil phase into the main emulsification pot and heat and stir to 85±2°C. After fully dissolving, add the raw materials of the water phase to the auxiliary emulsification pot and heat to 60±2°C. Slowly add the water phase to the oil phase, homogenize at 3000rpm for 20min, cool to 45±2°C, keep stirring for 30min, ...

Embodiment 3

[0026] Embodiment 3 anti-aging emulsion, in total amount 1000g.

[0027] Premixed phase: 25 g of a 3% aqueous solution of hydroxyethyl cellulose.

[0028] Water phase: 621.8g of deionized water, 80g of glycerin, and 30g of butanediol.

[0029] Oil Phase: Cetearyl Ether - 212g, Cetearyl Ether - 2115g, Hydrogenated Polyisobutylene 50g, Dimethicone 30g, Caprylic / Capric Triglyceride 30g, Ethylhexyl Palmitate 40g, 5g butylmethoxydibenzoylmethane, 5g 4-methylbenzylidene camphor, 5g phenoxyethanol.

[0030] Post-addition phase: 30g of purified polypeptide from pumpkin seeds, 1g of triethanolamine, 5g of vitamin E acetate, 5g of nicotinamide, 10g of water-soluble azone / PEG-40 hydrogenated castor oil, and 0.2g of essence.

[0031] Production process: Make 3% aqueous solution of hydroxyethyl cellulose with deionized water, and disperse it with a dispersing disc for 1 hour to obtain a premixed phase. Add the premixed phase to the water phase, heat and stir in the main emulsification p...

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Abstract

The invention belongs to the technical field of cosmetics, and specifically relates to a firming and revitalizing night cream containing pumpkin seed polypeptide, an anti-aging emulsion and a leave-in conditioner, and also relates to a preparation method of the pumpkin seed polypeptide and the above cosmetics. Pumpkin seed polypeptide includes pumpkin seed crude polypeptide and pumpkin seed purified polypeptide. The polypeptide is prepared by desalting with DA-201C macroporous adsorption resin, eluting with 75% ethanol-water solution, and freeze-drying. The amino acids in pumpkin seed peptides are rich in types and have antioxidant activity. The firming and revitalizing night cream contains 0.2-3% of pumpkin seed purified polypeptide, the anti-aging lotion contains 0.5-5% of pumpkin seed purified polypeptide, and the leave-in conditioner contains 1-5% of pumpkin seed crude polypeptide. The invention provides the application of high economic added value for the pumpkin seed meal, and the sensory evaluation results of the prepared cream, lotion and hair conditioner show that the skin care and hair care effects are remarkable.

Description

technical field [0001] The invention relates to the field of cosmetics, in particular to a cosmetic containing pumpkin seed polypeptide and its preparation method and application. Background technique [0002] As people pay more and more attention to the efficacy of cosmetics, active ingredients in cosmetic formulations have become a research hotspot. The natural product series of cosmetics has a broad market prospect because of its functional ingredients from plants, high safety and broad market prospects. In recent years, major cosmetics brands have launched a series of products containing peptides, such as Xiangyi Materia Medica Quadruple Extract Silk Series containing silk peptides, Estée Lauder La Mer contains yeast peptides, Olay Multi-effect Repair series contains wheat hydrolyzed protein peptides, etc. [0003] As a by-product of oil extraction, pumpkin seed meal has not been applied with high economic added value. However, the protein content is as high as 60%, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61K8/92A61K8/89A61K8/64A61Q19/08A61Q19/00A61Q5/12
Inventor 孙培冬唐蔚宁奇杨成曹光群
Owner JIANGNAN UNIV
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