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A kind of amphoteric carboxylic acid binuclear copper metal coordination polymer and preparation method thereof

A technology of coordination polymer and amphoteric copper carboxylate, which is applied in the field of chemistry to achieve the effects of mild reaction conditions, good detection effect, and easy access

Inactive Publication Date: 2019-05-21
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few studies on ligands containing carboxylic acid and quaternary ammonium salt groups, and there are few reports on the use of such metal coordination polymers as fluorescent probes for miRNA recognition.

Method used

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  • A kind of amphoteric carboxylic acid binuclear copper metal coordination polymer and preparation method thereof
  • A kind of amphoteric carboxylic acid binuclear copper metal coordination polymer and preparation method thereof
  • A kind of amphoteric carboxylic acid binuclear copper metal coordination polymer and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0040] 1. Preparation of Amphoteric Carboxylic Acid Binuclear Copper Metal Coordination Polymer

[0041] Step 1. Disperse 0.4mmol (166.0mg) ligand 1-(3,5-dicarboxylate benzyl)-4,4'-bipyridine bromide in methanol with a volume concentration of 99%, and use 0.1mM NaOH The pH of the solution was adjusted to 6.5 to obtain a solution of 1-(3,5-dicarboxylic acid benzyl)-4,4'-bipyridine bromide;

[0042] Step 2. Using the ether diffusion method, slowly diffuse ether into the 1-(3,5-dicarboxylate benzyl)-4,4'-bipyridyl bromide solution, and after standing for three days, a light green sodium salt is precipitated Crystal, collect crystal and obtain molecular formula as [Na(dcbb)(H 2 O)] n The intermediate product A; The intermediate product A is researched and analyzed, and the results are as follows: Elemental analysis C 38 h 50 CuN 4 o 20 4.5H 2 O, theoretical value: C 44.42, H 5.79, N 5.45; calculated value: C44.58, H 5.60, N 5.06; infrared spectrum (KBr disc, cm-1) ν3412(s),...

Embodiment 2

[0058] In this example, the amphoteric carboxylic acid binuclear copper metal coordination polymer obtained in Example 1 was used to study the recognition of five miRNAs.

[0059] The solubility of the metal coordination polymer of the present invention: under the condition of 25° C., 2.6 mg of the metal coordination polymer dissolves in 10 mL of water when it reaches a saturated state.

[0060] (1) Confirm the mature sequences of five miRNAs abnormally expressed in the peripheral blood of gastric cancer through the miRBase database, and artificially synthesize them as sequences to be detected, namely target RNAs. Considering the relative instability of RNA, we replaced the corresponding complementary strands of target RNAs with their complementary DNA (complementary DNA, cDNA), and fluorescently labeled the cDNAs sequences, namely probe DNAs. The five miRNAs interact as interfering sequences. To investigate whether metal coordination polymers could selectively recognize five...

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Abstract

The invention relates to an amphiprotic carboxylic acid binuclear copper metal coordinated polymer. The constitutional repeating unit of the amphiprotic carboxylic acid binuclear copper metal coordinated polymer is shown in formula I. The copper coordinated polymer is synthesized through reaction of 1-(3,5-dicarboxylic acid benzyl)-4,4-bromide bipyridine and copper salt. The binuclear copper metal coordinated polymer has high water solubility and can detect five kinds of high-expression-level miRNA in peripheral blood of patients with gastric carcinoma.

Description

technical field [0001] The invention relates to the field of chemistry, in particular to metal coordination polymers. Background technique [0002] microRNA (miRNA) is a class of endogenous RNA molecules composed of 18-25 nucleotides and involved in gene regulation. miRNA plays an important role in many biological processes (such as cell differentiation, proliferation, apoptosis and disease process, etc.). There are significant differences in the expression levels of miRNAs in different developmental stages and tissues. In recent years, scientists have found that the occurrence of many diseases is closely related to the expression level of miRNA. For example, in 2015, Shu & Liu et al. found that there were 5 kinds of miRNA (miR-185, miR-20a, miR-210, miR25 and miR- 92b) was significantly up-regulated, and it was deduced that these five miRNAs may be used as potential biomarkers for gastric cancer diagnosis. Therefore, miRNA, as a new biomarker for disease prediction and d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G83/00G01N21/64
CPCC08G83/008G01N21/6428G01N2021/6432
Inventor 陈金香覃亮陈文华刘叔文谢宝平孙斌
Owner SOUTHERN MEDICAL UNIVERSITY
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