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Lactobacillus agilis and application thereof

A lactobacillus and agile technology, applied in the field of microorganisms, can solve the problems of late development and utilization, lack of strains, etc., and achieve the effects of improving piglet immunity and disease resistance, promoting growth, and good growth performance

Inactive Publication Date: 2016-09-21
云南千村农牧科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the development and utilization of probiotics in my country started relatively late, and there is a lack of strains for probiotic production. Therefore, it is urgent to screen out more probiotic strains with excellent characteristics for production and application.

Method used

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  • Lactobacillus agilis and application thereof
  • Lactobacillus agilis and application thereof
  • Lactobacillus agilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation, cultivation and identification of porcine intestinal lactobacillus

[0030] (1) Isolation and cultivation of pig intestinal lactic acid bacteria

[0031] Aseptically collect healthy pig intestinal samples, remove the feces from the pig intestines, cut open the intestinal wall, rinse with normal saline, gently scrape the intestinal mucus with a blunt plastic sheet, and then use normal saline to wash the intestinal mucus in sequence 10, 10 2 , 10 3 , 10 4 , 10 5 , 10 6 Dilute, take 1mL of the dilution and pour it into the MRS medium with 1% (m / V) calcium carbonate (MRS medium was purchased from Guangdong Huankai Biotechnology Co., Ltd., 027315; calcium carbonate is domestic analytical grade), each dilution Pour into 2 plates and culture them anaerobically in a constant temperature incubator at 37°C for 48 hours. Pick a single colony with a typical calcium-dissolving circle, streak and pass on the MRS plate, and culture purely.

[0032] After add...

Embodiment 2

[0047] The biological characteristic screening of embodiment 2 bacterial classification

[0048] (1) Lactic acid bacteria growth curve formulation

[0049] Inoculate 15 strains of Lactobacillus in MRS broth with 6% inoculation amount, put them into an incubator, and culture them statically at 37°C. Samples were taken at 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24h, and the optical density values ​​(OD 600 value), the results are shown in Table 2, and the growth curve can be drawn according to the optical density value.

[0050] It can be seen from Table 2 that the cell density did not change significantly at the beginning of the culture. After 8 hours, the bacteria began to grow in large numbers and entered the logarithmic growth phase. After 20h-24h, the cell growth entered the stable phase from the logarithmic phase. Among them, LP14 had the best growth performance.

[0051] Table 2: Determination results of optical density values ​​of lactic acid bacteria growth ove...

Embodiment 3

[0075] Application of embodiment 3 Lactobacillus agili in piglet production

[0076] To evaluate the probiotic performance of Lactobacillus facilis on piglets, including observing its effects on piglet performance and immune function after feeding. For the animal experiment, 10 litters (10±2 heads / litter) of long × large binary hybrid piglets with similar body weight, litter size, sow parity, and birth date were selected and randomly divided into two treatments, namely the control group (feeding basis diet) and the experimental group (feeding basal diet + Lactobacillus agili). Insert Lactobacillus facilis strains into MRS broth medium in an aseptic manner, and anaerobically cultivate them in a constant temperature biochemical incubator at 37°C until the bacterial cell content reaches 10 10 CFU / mL, stored at 4°C. In the experimental group, the amount of Lactobacillus agilis added to the basal diet was 2×10 11 CFU / kg. The experiment was divided into two stages: in the first st...

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Abstract

The invention relates to lactobacillus agilis and an application thereof and belongs to the technical field of microorganisms. The bacterial strain is a lactic acid bacterium which is separated from a normal microbial flora in intestinal tracts of a healthy pig. By means of Gram staining microscopy, physiological biochemical test and 16s-rRNA sequence analysis, the bacterial strain is identified to be a strain of lactobacillus agilis. Through growth curve drawing, gastrointestinal tract stress resistance environment test and in-vitro enteroadhesive test screening, the lactobacillus agilis strain shows the best biological characters when being compared with other lactic acid bacteria separated from the intestinal tract. The lactobacillus agilis can be applied as a feed additive in production of piglets when being completely cultured. The lactobacillus agilis can promote growth and increase utilization rate of the feed, can reduce diarrhea ratio, improve animal immunity level and improve disease resistance, can effective replace antibiotics in feed additives. The invention provides an excellent bacterial strain for probiotics, and has important significance of improving economic benefit in pig breeding and developing healthy breeding of pigs.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a lactobacillus agili and the application of the culture of the strain in piglet production. Background technique [0002] Antibiotics as feed additives have made great contributions to the rapid development of animal husbandry, but their widespread and long-term use will lead to problems such as residues and bacterial resistance. With the growing awareness of the disadvantages of antibiotics, the European Union, Japan, South Korea and other countries have banned the use of antibiotics in animal feed. Therefore, it has become more and more important to find new agents that are safe, growth-promoting, residue-free, and drug-resistant as substitutes for antibiotics. [0003] Probiotics, also known as biogen or probiotics, refers to the use of microecological principles, the use of probiotics or probiotic growth-promoting substances that are beneficial and harmless to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23K10/18A23K50/30C12R1/225
CPCC12N1/04C12N1/205C12R2001/225
Inventor 邓绍贤张以芳杨洁邓绍纲郑锦玲张云娟张娜娜柴俊
Owner 云南千村农牧科技集团有限公司
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