Feeding antibacterial dodecapeptide from pig lysozyme and preparation method of feeding antibacterial dodecapeptide

An antibacterial peptide and lysozyme technology, applied in the field of bioengineering, can solve the problems of limited application and no bacteriostatic effect, and achieve the effects of simple preparation process and high product purity
CN105950587AActive Publication Date: 2016-09-21JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2016-09-21

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Abstract

The invention discloses a feeding antibacterial dodecapeptide from pig lysozyme and a preparation method of the feeding antibacterial dodecapeptide, belonging to the field of bioengineering. The preparation method comprises the steps of preparation of a crude extracting solution, separation of a gel filtration chromatography column Superdux Peptide 10 / 300GL and separation and purification of a reversed-phase column Phenomenx luna C18. Through LC-MS (Liquid Chromatography-Mass Spectrometry) identification, the amino acid sequence of the antibacterial dodecapeptide is G-V-S-L-A-N-W-V-C-L-A-K; the antibacterial peptide belongs to an alkaline peptide, has remarkable resistance for gram-negative bacteria and is good complementation and expansion for an antibacterial spectrum of the pig lysozyme; through comparison with an antibacterial peptide database and the like, the antibacterial peptide has not been reported. A preparation process disclosed by the invention has the characteristics of simplicity and high efficiency; the purity of a product can reach 95 percent or higher through mass spectrum identification.
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Description

technical field

[0001] The invention relates to feed antibacterial dodecapeptide derived from porcine lysozyme and a preparation method thereof, belonging to the field of bioengineering. Background technique

[0002] For decades, antibiotics have played a positive role in preventing and treating animal diseases, promoting animal growth, and increasing the output of livestock and poultry products by inhibiting the proliferation of pathogenic microorganisms and reducing the toxic effects of their metabolic toxins on animals. However, the long-term use of antibiotics, especially the use of anti-disease drugs and growth-promoting additives in the breeding process, will cause excessive drug residues, environmental pollution in the production area, and lead to a decline in the quality of muscle and processed products. In addition, the long-term use of antibiotics as feed additives in poultry and livestock can cause some bacterial flora to become drug-resistant strains, thus making...

Claims

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