Serratia marcescens rz 21-C6 and its application
A RZ21-C6, Serratia marcescens technology, applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of insufficient utilization of cheap biomass carbon sources, lack of utilization ability of straw hydrolyzate, poor genetic stability of strains, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0033] ARTP mutagenesis and screening of high prodigiosin bacterial strains of embodiment 1
[0034] (1) Strain mutagenesis: the lethality curve of the original strain of Serratia marcescens induced by plasma at normal pressure and room temperature is as follows: figure 1As shown (the original strain was isolated from a commercially available photosynthetic bacteria agent for aquatic products, numbered RZ 21, see the literature for details: Min Taoling, Yang Qiyin, Guo Xiaoxiao, etc. A strain of Serratia marcescens in the photosynthetic bacteria agent Separation and identification. Anhui Agricultural Sciences, 2006,34(22):5837-5838,5841.), plasma is relatively strong to Serratia marcescens, and it will kill nearly 60% of the thalli for 15s; After 60s, the lethal rate reaches over 90%; after 240s, the lethal rate reaches 100%. The mutation itself is random, and the relationship between its lethality and positive mutations is not very clear, but according to literature reports,...
Embodiment 2
[0038] Embodiment 2 mutant strain physiological performance analysis
[0039] (1) TEM observation of cell morphology changes: the Serratia marcescens mutant strain RZ 21-C6 was observed under a transmission electron microscope, and its morphology had significant changes compared with the starting strain RZ21 ( Image 6 ). The cell volume of the mutant bacteria is significantly larger, and the specific surface area of the cells is larger; the cell wall becomes very thin, the cell surface becomes round and transparent, and the permeability increases, which will be very conducive to the secretion of intracellular secondary prodigiosin To the outside of the cell, it reduces the harmful effect on itself, and it is also beneficial to the separation and purification process of the later product.
[0040] (2) Analysis of cell membrane phospholipid fatty acid composition: As can be seen from Table 1, the fatty acid composition in the cell membrane phospholipid of the mutant strain R...
Embodiment 3
[0046] Embodiment 3 Mutant bacteria and original bacteria are compared to the utilization situation of different carbon sources
[0047] (1) Investigation of the utilization of four basic carbon sources (sucrose, glucose, xylose and arabinose) by the mutant strain RZ 21-C6 is shown in Table 2. Compared with the original strain, the production of prodigiosin in the sucrose-containing medium of the mutant strain was increased by 2.59 times, and the production efficiency was increased by 2.63 times, indicating that the screened strain not only has higher pigment production, but also has improved production efficiency , which is consistent with the conclusions drawn from the physiological changes in Example 2. In the xylose-containing medium, the amount of prodigiosin produced by RZ 21-C6 was 17.21 times higher than that of RZ 21, reaching 3.27g / L. Compared with the original strain which cannot use glucose to produce pigment, the mutant strain RZ 21-C6 can use glucose well to pro...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More