Tumor cell targeting mesoporous silicon nanometer assembly and preparation method for same

A nano-assembly and tumor cell technology, applied in the field of mesoporous silicon nano-assembly and its preparation, can solve the problems of drug leakage, side effects, uncontrollable drug release, etc., to promote uptake, enhance drug concentration, and solve controllable release poor sex effect

Inactive Publication Date: 2016-10-12
SHANGHAI INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, MSNs also have the problem of uncontrollable drug release

Method used

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  • Tumor cell targeting mesoporous silicon nanometer assembly and preparation method for same
  • Tumor cell targeting mesoporous silicon nanometer assembly and preparation method for same
  • Tumor cell targeting mesoporous silicon nanometer assembly and preparation method for same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation method of lipid mesoporous silicon core-shell nano-assembly modified by hyaluronic acid

[0026] (1) Preparation of mesoporous silicon nanoparticles: Take 3.6mL of 2mol / L NaOH solution, add it to 800mL water, heat to 80°C, and then dissolve 2.4g of cetyltrimethylammonium bromide CTAB in ethanol The solution was added into hot water at 80° C., and reacted for 2 hours at a rotation speed of 700 rpm. Then slowly add tetraethyl orthosilicate TEOS 8mL, and continue to react for 8h. Add an equal volume of ethanol to the reaction system, and after a flocculent precipitate precipitates, filter, dissolve the precipitate in a mixture of ethanol: hydrochloric acid = 9:1, and reflux at 80°C for 8 hours to effectively remove the surfactant , and then centrifuged and dried to obtain mesoporous silicon nanoparticles (MSNs) with an average particle diameter of about 85 nm.

[0027] (2) Disperse the prepared mesoporous silicon with ethanol, mix it with 10 mg / mL doxorubicin...

Embodiment 2

[0035] Preparation method of lipid mesoporous silicon core-shell nano-assembly modified by hyaluronic acid

[0036] (1) Prepare mesoporous silicon nanoparticles according to the method described in Example 1, disperse the prepared mesoporous silicon with ethanol, mix it with 15mg / mL doxorubicin (DOX) solution, and keep away from light Stir for 8h to make full contact. Centrifuge after 8 hours, wash with PBS buffer several times, and when the washing solution is colorless, the drug-loaded mesoporous silicon DOX@MSNs are obtained. The washing buffer and the residual DOX solution were collected, and the DOX concentration of the residual solution was measured using an ultraviolet spectrophotometer at a wavelength of 480 nm, and then the drug loading capacity of MSNs was obtained. It was determined that the final drug loading capacity of MSNs was 12.68mg, that is, 12.68mg DOX was loaded per 100mg MSNs.

[0037](2) Weigh 30 mg of hydrogenated soybean lecithin, 7.5 mg of cholestero...

Embodiment 3

[0042] (1) According to Example 1, the prepared mesoporous silicon was dispersed with ethanol, mixed with 10-hydroxycamptothecin solution, and stirred for 24 hours in the dark to make it fully contact. After 8 hours, it was centrifuged, washed with PBS buffer, and dried in vacuum to obtain MSNs loaded with 10-hydroxycamptothecin, which were stored in the dark at room temperature.

[0043] (2) Weigh 25 mg of hydrogenated soybean lecithin, 6.25 mg of cholesterol and 1.64 mg of distearoylphosphatidylethanolamine, respectively, and dissolve them in 20 mL of chloroform. Rotary steam at 30°C to form a film. The lipid film was hydrated with 40 mL of PBS buffer dissolved in 10 mg of mesoporous silicon, and hydrated using a rotary evaporator under a water bath condition of 60 °C. The homogenizer was homogenized under high pressure for 5 minutes under the condition of 1000 Pa to obtain liposomes loaded with mesoporous silicon.

[0044] (3) Weigh 26mg HA (600-800KDa) into 10mL distille...

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Abstract

The invention discloses a mesoporous silicon nano assembly with tumor cell targeting and a preparation method thereof. The specific steps of the present invention are as follows: (1) first use the surfactant cetyltrimethylammonium bromide as a template, and tetraethyl orthosilicate as a silicon source, under alkaline conditions, at a temperature of 75-85°C The crude product is synthesized, and the surfactant is removed after post-treatment to obtain mesoporous silicon; (2) the mesoporous silicon and the drug are mixed in ethanol to obtain the drug-loaded mesoporous silicon; (3) the drug-loaded mesoporous silicon is treated with phosphate The buffer solution PBS is dispersed, added to the liposome membrane, first hydrated, and then homogenized to obtain the liposome-wrapped mesoporous silicon nanoparticles; (4) react the liposome-wrapped mesoporous silicon nanoparticles with HA Get the target material. The preparation method of the present invention is simple, and the obtained mesoporous silicon nano assembly can enhance the uptake of tumor cells to drugs from two aspects of targeting and preventing drug diffusion, so as to achieve the effect of treating tumors, and has obvious advantages in the treatment of tumors.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and in particular relates to a mesoporous silicon nano assembly with tumor cell targeting and a preparation method thereof. Background technique [0002] In recent years, malignant tumors have become a major disease that threatens human life and health, and the morbidity and mortality have been increasing year by year in recent years. Therefore, the research and development of anti-tumor drugs has become the top priority of governments, research institutions and pharmaceutical companies, and the research of anti-tumor drugs has also paid great attention. At present, although there are many ways and means to treat tumors, chemotherapy is still the main method for treating tumors. However, this method has many problems, such as large side effects, poor selectivity, strong drug resistance, and more importantly, in conventional chemotherapy methods, chemotherapy drugs are distrib...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/04A61K47/48A61K31/704A61K31/4745A61K31/337A61P35/00
CPCA61K9/127A61K31/337A61K31/4745A61K31/704A61K47/02
Inventor 吴范宏王智慧田永峰于艳艳甘莉
Owner SHANGHAI INSTITUTE OF TECHNOLOGY
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