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Method for preparing broad-spectrum long-acting antibacterial anti-adhesive chitosan nano silver composite gel coating layer

A chitosan nano and composite gel technology, applied in coatings, pharmaceutical formulations, catheters, etc., can solve the problems of inability to achieve long-term antibacterial, inability to kill germs, etc., and achieve broad-spectrum multifunctional antibacterial and excellent antibacterial properties. Adhesion, improve the effect of bonding stability

Inactive Publication Date: 2016-10-12
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a simple hydrophilic coating cannot achieve long-term antibacterial ability, nor can it kill germs around the implant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Add 50ml of acetic acid buffer solution (pH=4.0) into a 50ml small beaker, stir magnetically, slowly add 0.05g of polyvinyl alcohol and 0.0005g of AgNO 3 , the concentration is 1mg / ml and 0.1mg / ml, after stirring and dissolving, slowly add 0.50g chitosan, the concentration is 10mg / ml, after the viscosity increases, turn on the stirring to the maximum, and continue stirring for 2h, which is the coating solution. PET sheet is cut into 1.5×2.5cm by blade 2 Size, used as a base material, and then cleaned with acetone, ethanol and ultrapure water in sequence for 15 minutes, N 2 blow dry. The cleaned PET was treated in 5mg / ml PEI (polyimide) for 30min; in 5mg / ml PAA (acrylic resin emulsion) for 30min; after mixing 10mg / ml EDC (dichloroethane) and 20mg / ml NHSS (N-hydroxysuccinimide) in MES (2-(N-morpholine) ethanesulfonic acid monohydrate) buffer (pH=5.6) for 6h, N 2 blow dry. The contact angles of untreated PET and PET treated with PEI, PAA and EDC / NHS were 70.3±0.5°, 80...

Embodiment 2

[0020] Add 50ml of acetic acid buffer solution (pH=4.0) into a 50ml small beaker, stir magnetically, slowly add 0.10g of polyvinyl alcohol and 0.0010g of AgNO 3 , the concentration is 2mg / ml and 0.2mg / ml, after stirring and dissolving, slowly add 0.25g chitosan, the concentration is 5mg / ml, after the viscosity increases, turn on the stirring to the maximum, continue stirring for 2h, that is the coating solution. The polylactic acid film was used as the substrate, and the cleaning, pretreatment and coating methods were the same as those of PET in Example 1, and then the coating was reduced in situ in 8 mg / ml ascorbic acid solution for 6 hours. The atomic force microscope observed the morphology and found that the surface has a very low roughness, and the RMS is 3.72±0.34nm. The results of transmission electron microscopy showed that the particle size distribution of nano-silver particles was 7-11nm, with an average of 9nm. The bactericidal performance of the film layer was tes...

Embodiment 3

[0022] Add 50ml of acetic acid buffer solution (pH=4.0) into a 50ml small beaker, stir magnetically, slowly add 0.25g of polyvinyl alcohol and 0.0015g of AgNO 3, the concentration is 5mg / ml and 0.3mg / ml, after stirring and dissolving, slowly add 0.35g chitosan, the concentration is 7mg / ml, after the viscosity increases, turn on the stirring to the maximum, continue stirring for 2h, that is the coating solution. Glass is as base material, and cleaning and pretreatment method are the same as the PET in embodiment 1, and film coating method adopts the method for spin coating, spin coating 10s at 1000rad / min earlier, then spin coating 2min under 2500rad / min, repeat operation, spin Apply 5 times. The coating was then reduced in situ for 12 h in a 10 mg / ml ascorbic acid solution. The thickness of the film layer measured by the cross-sectional field emission scanning electron microscope is 2.55±0.38 μm. The static contact angle test film layer shows a certain degree of hydrophilici...

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Abstract

The invention discloses a method for preparing a broad-spectrum long-acting antibacterial anti-adhesive chitosan nano silver composite gel coating layer, and is characterized in that with use of solutions of chitosan, silver nitrate and polyvinylpyrrolidone, the nano composite gel coating layer having hydrophilic surface and being stable in an aqueous environment is obtained by base material pretreatment, physical coating and in-situ reduction; the coating layer has excellent broad-spectrum bacterial adhesion resistance and sterilization abilities, and has excellent cellular biocompatibility. The method is simple and rapid in process, mild in conditions, easy to achieve by spin coating, dip coating, spray coating and other methods which can be implemented industrially, is wide in applicable scope, and can effectively improve antibacterial property, biocompatibility and lubricating property of surfaces of medical devices.

Description

technical field [0001] The invention relates to the technical field of new materials, in particular to a method for preparing a broad-spectrum, long-acting antibacterial and anti-adhesion chitosan nano-silver composite gel coating. Background technique [0002] Pathogenic microorganisms such as bacteria and fungi often cause pathological changes in body tissues, seriously threatening human physical and mental health. According to the statistics of World Health Organization (WHO) in 1998, there were 52 million deaths in the world in 1995, of which about 33% were caused by bacterial infections, and this ratio is still increasing today. Therefore, the development of antibacterial materials and products has received more and more attention. Nowadays, the main way to fight infection after clinical surgery and medical implants is to use antibiotics. However, with the extensive use of antibiotics, the resistance of bacteria has also greatly increased, especially once biofilms are ...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/30A61L27/54A61L27/52A61L29/08A61L29/10A61L29/16A61L29/14A61L31/10A61L31/08A61L31/16A61L31/14
CPCA61L27/34A61L27/52A61L27/54A61L29/085A61L29/145A61L29/16A61L31/10A61L31/145A61L31/16A61L2300/104A61L2300/232A61L2300/404A61L2300/424A61L2300/606A61L2400/10A61L2400/18A61L2420/02C08L5/08C08L29/04
Inventor 王佰亮陈浩刘慧华叶子徐青文
Owner WENZHOU MEDICAL UNIV
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