Novel ink melanin extraction method

Abstract

The invention discloses a novel ink melanin extraction method, which comprises the following steps: (1) microwave removal of polysaccharides; (2) ultrasonic removal of fats; (3) enzymatic hydrolysis of proteins: adding a disodium hydrogen phosphate-potassium dihydrogen phosphate buffer solution with a pH value of 7.5 to 8.5 into precipitates obtained in step (2) according to a material-solution ratio of 2.5 to 4.5g/ml, regulating the temperature to 45 to 55 DEG C after uniform stirring, adding 3.2 to 4.4 percent of plant proteinase, performing reaction for 1 to 1.5h, regulating the pH value of the buffer solution to 4.9 to 6.1, removing supernatant after precipitates are separated out, and rinsing with sterile water and drying the precipitates, wherein the plant proteinase is gingko leaf proteinase or myrica rubra leaf proteinase. Compared with the prior art, the method has the beneficial effects that cephalopod processing residues, i.e. ink bags, are adopted as extraction raw materials, so that the cost is low, and environmental pollution is reduced; a crude plant protein enzyme solution with high enzyme activity is adopted to remove the proteins, so that the time consumption is low, the efficiency is high, and an obtained ink melanin is high in purity and solubility.

Description

technical field [0001] The invention belongs to the technical field of melanin extraction, and in particular relates to a new method for extracting melanin. Background technique [0002] Melanin is a kind of natural pigment that widely exists in organisms. It exists in plants, animals and microorganisms. It is generally insoluble in water and firmly combined with proteins, sugars or other components in biological tissues. Difficult to separate and purify. According to the color, chemical composition (nitrogen-containing or sulfur-containing) and formation pathway of melanin, melanin can be divided into eumelanin (mainly black and brown nitrogen-containing pigments, oxidized by tyrosine, dopa, dopamine, tyramine, etc. Polymerized), phaeomelanin (with cysteine ​​dopa as the basic structural unit, containing sulfur in the molecule, generally brown or red) and isomelanin (usually black, produced by polyphenols in the presence of polyphenol oxidase According to the alkali hydro...

AI Technical Summary

Problems solved by technology

In 2014, the catch of cephalopods in my country reached 155.52×10 4 t, however, the cephalopod ink sacs, which are the main source of melanin, are generally regarded as the leftovers of cephalopod processing and are discarded or processed into low-value feed, resulting in a huge waste of resources and serious environmental pollution

Like most natural melanin, black melanin has biological activities such as enhancing immune function, anti-tumor, anti-oxidation, anti-aging and antibacterial, but also has the disadvantages of being insoluble in water and difficult to separate and purify, which greatly limits its application in medicine, Applications in food and other fields

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