Method for quick detection of toxoflavin in food

A detection method and technology for food poisoning, which are applied in measurement devices, instruments, scientific instruments, etc., to achieve the effects of being environmentally friendly, easy to popularize and apply, and reducing interference

Inactive Publication Date: 2016-10-12
ZHEJIANG INST OF QUALITY INSPECTION SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, there are few reports on the research on fo

Method used

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  • Method for quick detection of toxoflavin in food
  • Method for quick detection of toxoflavin in food
  • Method for quick detection of toxoflavin in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Draw the calibration curve of response peak area-toxoflavin concentration.

[0031] (1) Weigh 0.0100g of toxin standard product into a 10mL volumetric flask, dissolve in methanol, set the volume to the mark, and prepare a 1.0mg / mL standard mother solution. Stored in -18°C refrigerator, it can be stored for 3 months. Before use, take it out and let it stand at room temperature, take 200μL of the standard mother solution in a 10mL volumetric flask, dilute to the mark with water, shake well, and prepare a 20.0μg / ml intermediate solution for use. Take 25μL, 50μL, 100μL, 200μL, 500μL, 1.0mL intermediate use solution and 50μL, 100μL standard mother solution in 10ml volume, and dilute to the mark with water. Prepare a series of standard solutions with concentrations of 0.05, 0.1, 0.2, 0.4, 1.0, 2.0, 5.0, and 10.0 μg / ml.

[0032] Simultaneously, this part has been optimized to constant volume solution, has compared the methanol aqueous solution of volume fractio...

Embodiment 2

[0034] Embodiment 2: The present embodiment detects toxanthin in fermented cornmeal by the following method:

[0035] (1) Sample extraction conditions: Weigh 20-25 g of fermented cornmeal and mix with 100 mL of distilled water, extract by ultrasonic for 20 min, take the extract, and centrifuge at 5000 r / min for 5 min to obtain the supernatant.

[0036] (2) Sample enrichment and purification: take 5.0 mL of the supernatant obtained in step 1, and pass it through an HLB (6cc, 200mg) cartridge for purification (before loading the sample, the cartridge is activated with 5mL methanol and 5mL water respectively); After the flow is over, rinse the small column with 5mL of water, squeeze it dry, and discard all the effluent. Then use 5.0mL of 80% methanol aqueous solution for elution to obtain the eluate, dry the eluate with N2, then dilute to volume with 1.0mL pure water, vortex to dissolve to obtain the supernatant.

[0037](3) Chromatographic analysis conditions: the upper machine...

Embodiment 3

[0040] Example 3: In this example, in Example 2, a positive fermented cornmeal sample was prepared by adding toxin standard substance into cornmeal at the same time, and the same method was used for detection. Wherein in step 1, this part has been tested to extraction reagent, has selected distilled water, different volume fraction methanol water (10%, 20%, 40%, 60%, 80%), different volume fraction acetonitrile water (10%, 20%) %, 40%, 60%, 80%) compared the extraction efficiency of the positive sample fermented cornmeal, when implementing this part of the test, directly pipette 3.0ml of the supernatant obtained after the extraction was dried and concentrated by N2 to go on the machine Analysis (without purification by solid-phase extraction cartridges, thereby excluding the influence of cartridge recovery). It was found that the extraction with distilled water, the volume fraction of acetonitrile water was 80% (attached Figure 9 ) and methanol water volume fraction 10% (att...

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Abstract

The invention discloses a method for quick detection of toxoflavin residues in food based on solid-phase extraction-high performance liquid chromatography. A food sample is subjected to pure water dissolution and ultrasonic extraction, the sample is purified with a solid-phase extraction column (HLB) to achieve enrichment of the target analyte toxoflavin, the solid-phase extraction column is eluted with aqueous methanol of 80% as eluant, blow drying is conducted by means of N2, and supernate is obtained after water dissolution and volume metering. Due to the pretreatment condition, during detection of toxoflavin residues in the sample, influences of disrupting compounds on accurate metering of toxoflavin are effectively reduced, and adverse effects of solvent effect on toxoflavin chromatographic behavior are eliminated. Methyl alcohol and water gradient elution is conducted on the mobile phase, so that reservation of toxoflavin in a reversed phase column is enhanced, effective separation of toxoflavin and other interferents is achieved during chromatographic analysis of the sample, and then accurate metering and analysis are realized. The method is low in detection cost, high in accuracy, sensitivity and result reproducibility and suitable for detection of toxoflavin residues in various food, and no toxic reagent is used so that environment friendliness is realized.

Description

technical field [0001] The invention relates to the technical field of food safety, in particular to a rapid detection method for food poisoning flavins. Background technique [0002] The structural formula of toxoflavin (CAS:84-82-2) is: [0003] [0004] It is a bacterial toxin that can cause food poisoning produced by Pseudomonas cocos subsp. Common contaminated foods are mainly fermented cereal products, potato products and white fungus. Food poisoning by Pseudomonas cocos subspecies fermented rice noodles mostly occurs in summer and autumn (mainly from May to August) during the rainy and humid seasons. Improper food storage causes Pseudomonas cocos to multiply and grow rapidly, and food contaminated by it is eaten It can cause poisoning, manifested as symptoms such as epigastric discomfort, nausea, vomiting, dizziness, and general weakness. In severe cases, hepatosplenomegaly, subcutaneous hemorrhage, hematuria, and shock may occur, and the mortality rate is as hig...

Claims

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Application Information

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IPC IPC(8): G01N30/89
CPCG01N30/89G01N2030/027
Inventor 李红艳翁晨辉黄海智沈潇冰盛华栋张东雷张慧王瑾
Owner ZHEJIANG INST OF QUALITY INSPECTION SCI
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