Combined method for culturing microalgae and denitration of industrial exhaust gas
A technology of industrial waste gas and combined methods, which is applied in separation methods, chemical instruments and methods, biosynthesis, etc., can solve the problems of low solubility, impact, difficult breeding water recycling, etc., and achieve improved efficiency, low breeding costs and high utilization rate high effect
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Embodiment 1
[0128] This example is used to illustrate "the effect of adding EM bacteria on microalgae photoautotrophy".
[0129] Use BG11 medium (add nutrients according to Table 1, and the culture medium is not sterilized) to cultivate Chlorella (from Sinopec Microalgae Species Bank, No. Chlorella sp.RIPP-1), and control the temperature at 20-30°C Between, pass compressed air and CO 2 Cultivate, when the pH of the algae solution is >10, pass CO 2 , stop feeding CO when the pH of the algae solution is 2 . During the cultivation process, natural sunlight is used for cultivation, and the light intensity during the day can reach up to 60,000 lux, and the OD of the algae liquid is detected every day 680 The value was harvested after 14 days of continuous culture, and the CO2 was stopped 1 day before the end of the culture. 2 After the cultivation is completed, the algae mud and algae culture residue are obtained through centrifugal separation. The growth curve of microalgae is shown in f...
Embodiment 2
[0132] Chlorella sp. RIPP-1) was cultivated in BG11 medium (add nutrients according to Table 1, and the culture medium was not sterilized), and 2 g / L of Glucose, control the temperature between 20 ~ 30 ℃, pass compressed air and CO 2 Cultivate, when the pH of the algae solution is >10, pass CO 2 , stop feeding CO when the pH of the algae solution is 2 . During the cultivation process, natural sunlight is used for cultivation, and the light intensity during the day can reach up to 60,000 lux, and the OD of the algae liquid is detected every day 680 value, the growth curve of microalgae see figure 2 . Among them, the amount of EM added is 3.6×10 6 pcs / L algae liquid, the bacterial count of the algae liquid monitored during the breeding process is 6 1 / mL algae liquid, harvested after 14 days of continuous culture, stop feeding CO 1 day before the end of culture 2 Flue gas, and the pH of the algae liquid is naturally raised to 9.4, and then the cultivation is ended, and the ...
Embodiment 3
[0134] The difference between this embodiment and embodiment 2 is only that the amount of EM added is 1.8×10 7 pc / L algae liquid. During the breeding process, the bacterial count of the algae liquid should be monitored 7 per mL of algae liquid, the pH of the algae liquid naturally rose to 9.3 at the end of the culture. The growth curve of microalgae is shown in figure 2 .
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