Microcystic toxin-LR electrochemical detection method with amplified graphene signals

A technology of microcystin and signal amplification, applied in electrochemical variables of materials, measuring devices, scientific instruments, etc., can solve the problems of high cost, high cycle, complex process, etc., and achieve good electrical conductivity, sensitive electrochemical signals, and more The effect of electrochemically active area

Inactive Publication Date: 2016-10-26
TONGJI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Chinese patent 200810242879.5 discloses the preparation and application of a microcystin-LR quantitative rapid detection sensor, but this patent is an electrochemical sensor that uses biological antibodies as the selective mecha

Method used

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  • Microcystic toxin-LR electrochemical detection method with amplified graphene signals
  • Microcystic toxin-LR electrochemical detection method with amplified graphene signals

Examples

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Example Embodiment

[0032] Example 1

[0033] A graphene signal amplification microcystin-LR electrochemical detection method, in which the specific manufacturing process of the sensor involved is as follows:

[0034] (1) First, pre-treat the gold electrode. The processing method is as follows: Place the surface of the gold electrode on a freshly configured piranha (H 2 SO 4 / H 2 O 2 =7:3v / v) Soak in the lotion for 10-15 minutes, after taking it out, rinse with ultrapure water, and use aluminum oxide with particle size of 1.0μm, 0.3μm, and 0.05μm in sequence with the word "8" Carefully polish to a smooth surface. Then rinse the aluminum oxide on the surface of the electrode with ultra-pure water, and place the electrodes in ultra-pure water, ethanol, and ultra-pure water for 5 to 10 minutes. After the ultrasound is completed, take out the electrode immediately, use it as the working electrode, the platinum wire electrode as the counter electrode, and the SCE as the reference electrode to perform cycl...

Example Embodiment

[0037] Example 2

[0038] Before measuring the concentration of MC-LR, take out the prepared MCH / Au NPs / Au electrode, and drop 10μL of endonuclease (DNase I) reaction buffer (pH=7.0, containing 100mmol / L) on the surface of the electrode. NaCl), 5μL of graphene-aptamer complex, 3μL of MC-LR at the test concentration, 2μL of DNase I solution (1000U / mL), put the electrode in a biochemical incubator at 30°C for one hour take out. Gently rinse the electrode surface with ethanol and ultrapure water to remove the weakly bound graphene. The electrode was then dried under a nitrogen atmosphere, and then taken out for DPV detection. The electrochemical detection was carried out in a 20 mmol / L phosphate buffer solution (pH=7.0) containing 5 mmol / L ferrocene formic acid and 0.1 mol / L sodium perchlorate.

[0039] The change of current and the concentration of MC-LR are at 1.0×10 -12 ~1.0×10 -10 There is a good linear relationship in the range of mol / L, the correlation coefficient is about 0....

Example Embodiment

[0040] Example 3

[0041] Put the prepared electrochemical aptamer sensor into 1.0×10 -11 mol / L MC-LR and the concentration of 1.0×10 -9 mol / L interfering substances are mixed in pairs for determination. The interference experiments of three common environmental pollutants of omethoate, glyphosate, paraquat, dimetrodin, acetamiprid and trichlorfon on the determination of MC-LR were investigated. Using the test conditions in Example 2 to determine the current response, the results of the study show that other interferences with a concentration of 100 times the MC-LR have an impact on the MC-LR current by less than 10.0%. It can be seen that the prepared aptamer sensor has high selectivity to MC-LR.

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Abstract

The invention relates to a microcystic toxin-LR electrochemical detection method with amplified graphene signals. The method comprises the following steps: (1) dropwise adding a mixed solution with a ratio of an endonuclease reaction buffer solution: a graphene-aptamer compound: a microcystic toxin-LR solution to be detected: an endonuclease solution of (9-11):(4-6):(2-4):(1-3) on the surface of an MCH/Au NPs/Au electrode; (2) culturing the MCH/Au NPs/Au electrode for 1 to 2 hours at a temperature of 25 to 35 DEG C, slightly washing the electrode surface by ethanol and ultrapure water to remove weakly combined graphene, drying the electrode in nitrogen gas, taking the electrode out, carrying out electrochemical detection to measure the concentration of the microcystic toxin-LR solution. According to the provided method, a high sensitive electrochemical analysis technology and an aptamer with a high specific recognition performance are effectively combined to detect trace microcystic toxin-LR in the environment, the detection is highly sensitive and highly specific, moreover, the instruments are cheap and portable, the method is simple and easy to perform, and the results can be obtained quickly.

Description

technical field [0001] The invention relates to a microcystin-LR detection method, in particular to a microcystin-LR electrochemical detection method for graphene signal amplification. Background technique [0002] In recent years, environmental problems have been particularly serious, and blue-green algae outbreaks have occurred in many waters. Microcystins (MCs) are biotoxins produced by cyanobacteria in eutrophic water bodies, which are biologically active cyclic heptapeptide compounds composed of seven amino acids. There are many types of microcystins, and there are more than 90 confirmed isomers. Among them, Microcystin-LR (Microcystin-LR, MC-LR) is one of the isomers. Microcystin-LR exists most commonly in water and is also the most toxic microcystin. It is of great environmental significance to control and highly sensitively monitor the content of microcystin-LR in water. [0003] At present, the method for determining microcystin-LR mainly adopts traditional instr...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3277G01N27/48
Inventor 刘梅川王国强赵国华
Owner TONGJI UNIV
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