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Multivalent pneumococcus-ACYW135 meningococcal combined vaccine

One-ACYW135, meningococcal technology, applied in the field of multivalent pneumococcus-ACYW135 meningococcal combined vaccine, can solve the problems such as ineffectiveness, achieve good solubility, avoid side effects, and increase storage time

Pending Publication Date: 2016-11-09
BRAVOVAX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is a good countermeasure to prevent patients before they are attacked by bacteria.
[0005] 2. Limitations of Existing Technology
Immunization adjuvants fail to work if not used with appropriate vaccine, study finds

Method used

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  • Multivalent pneumococcus-ACYW135 meningococcal combined vaccine
  • Multivalent pneumococcus-ACYW135 meningococcal combined vaccine
  • Multivalent pneumococcus-ACYW135 meningococcal combined vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0064] 1. Preparation of ACYW135 meningococcal capsular saccharide

[0065] 1. Use CY medium to ferment the meningococcal strains of groups A, C, Y, and W135 at 37°C, inactivate the fermentation broth with formaldehyde, centrifuge to get the supernatant, and use cetyltrimethylammonium bromide ( CTAB) for precipitation. Then use 0.5-1M NaCl to dissociate the complex precipitation, add glacial ethanol to a final concentration of 25% (v / v) to precipitate nucleic acid, then add glacial ethanol to a final concentration of 75% (v / v), and precipitate to obtain rough sugar .

[0066]2. The above crude sugar was dissolved in sodium acetate solution, extracted 5-6 times with cold phenol, and finally concentrated by ultrafiltration to remove phenol residue, and refined sugar was obtained by 75% (v / v) ethanol precipitation, and stored at -20°C for later use.

[0067] 2. Preparation of pneumococcal polysaccharide

[0068] 1. Select seven serotypes (4, 6B, 9V, 14, 18C, 19F, 23F) of pneum...

Embodiment 1-2

[0087] The difference between this example and Example 1-1 is that the order of adding polysaccharides in this example is to add pneumococcal capsular saccharides and meningococcal capsular saccharides of groups A, C, Y, and W135 at the same time. There are no chromatographic peaks of meningococcal capsular saccharides of groups A, C, Y, and W135 after the sequence is reversed, which proves that adding pneumococcal capsular saccharides and capsular saccharides of groups A, C, Y, and W135 at the same time will form competition relationship, affecting the normal conjugation process of A, C, Y, W135 group meningococcal capsular saccharides, so this technical solution cannot be realized.

Embodiment 1-3

[0089] The only difference between this example and Example 1-1 is that the order of adding polysaccharides in this example is to add pneumococcal capsular saccharides first, and then add meningococcal capsular saccharides of groups A, C, Y, and W135. After the sequence was changed, there were no chromatographic peaks of meningococcal capsular saccharides of groups A, C, Y, and W135, which proved that adding pneumococcal capsular saccharides first and adding capsular saccharides of groups A, C, Y, and W135 in the original proportion would Competitive relationship is formed, which affects the normal conjugation process of meningococcal capsular saccharides of groups A, C, Y, and W135, so the technical solution cannot be realized.

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Abstract

The invention provides a multivalent pneumococcus-ACYW135 meningococcal combined vaccine, which contains 50-500 g / ml of pneumococcal capsular saccharide, 50-500 g / ml of A, C, Y and W135 meningococcal capsular saccharide per dosage; the pneumococcal serotype is one or more of the following serotypes: 1, 3, 4, 5, 6A, 6B, 7F, 8,9N, 9V, 12F, 14, 15B, 18C, 19A, 19F, 22F or 23F. Compared with the prior art, the combined vaccine provided by the invention is safe and reliable, and has good stability, and very wide application prospects.

Description

technical field [0001] The invention relates to a vaccine, in particular to a combination vaccine, especially a multivalent pneumococcus-ACYW135 meningococcus combination vaccine. Background technique [0002] 1. Bacterial meningitis [0003] The so-called bacterial meningitis can be caused by bacterial or viral infection. Many bacteria can cause this disease, among which meningococcus (also known as Neisseria meningitis) causes the most, followed by influenza bacillus, pneumococcus, Escherichia coli and other gram-positive bacilli, staphylococcus, listeria seedlings, anaerobic bacteria, etc. Bacterial meningitis is a serious infectious disease of the central nervous system. It is common in adults, especially in children. Children under 5 years old are most prone to this disease. It has usually occurred in sporadic cases over the past few decades. Symptoms in the early stages of infancy include: lethargy, fever, vomiting, refusal to eat or drink, increased crying, restle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/385A61K39/09A61K39/095A61K39/116A61K39/39A61K9/19A61K47/26A61P31/04
CPCA61K9/19A61K39/09A61K39/095A61K39/385A61K39/39A61K47/26A61K2039/55555A61K2039/55583A61K2039/6068A61K2039/62A61K2039/70A61K2300/00
Inventor 艾智武李津程超吴克
Owner BRAVOVAX
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