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A kind of molasses ethanol fermentation separation coupling method

A technology of ethanol fermentation and molasses, applied in the field of separation and coupling of molasses and ethanol fermentation, can solve problems such as pollution, achieve the effects of reducing product inhibition, reducing membrane fouling, increasing ethanol yield and

Active Publication Date: 2019-11-19
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a green molasses ethanol fermentation separation and coupling method, which solves the membrane fouling problem and product inhibition in the molasses ethanol fermentation separation and coupling process, and greatly improves the ethanol yield and sugar alcohol Conversion rate

Method used

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  • A kind of molasses ethanol fermentation separation coupling method
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Examples

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Effect test

Embodiment 1

[0029] Example 1: This example illustrates the preparation of alginate-immobilized Saccharomyces cerevisiae

[0030] Saccharomyces cerevisiae Saccharomyces cerevisiae AQ (CCTCC AY 2015008) was mixed with 2% sodium alginate solution, slowly dropped into 4% calcium chloride solution, and the formed white particles were solidified in calcium chloride solution for 4 h, and then washed 3 times with normal saline.

Embodiment 2

[0031] Example 2: This example illustrates the proliferation of alginate-immobilized Saccharomyces cerevisiae

[0032] The prepared immobilized cells were cultured in a 1 L fermenter for about 20-24 h at a temperature of 30 °C and a pH of 5.5.

[0033] Proliferation medium components were 1% yeast extract, 2% peptone, and 2% in situ pretreated molasses.

Embodiment 3

[0034] Embodiment 3: This embodiment illustrates the coupling of fermentation and separation of alginate immobilized S. cerevisiae

[0035] Proliferated immobilized cells were inoculated in the fermentation medium according to 50% of the volume of the fermenter at a temperature of 30 °C.

[0036] pH 5.5. The components of the fermentation medium were 18% in-situ pretreated molasses, 0.2% potassium dihydrogen phosphate, 0.3% magnesium sulfate, 1% ammonium sulfate, and 1% yeast extract.

[0037] When the ethanol concentration in the fermenter reaches 6% (v / v), the membrane separation is started, and the permeate is collected in the cold trap.

[0038] The retentate is returned to the fermenter to continue as culture medium. When the total sugar concentration in the fermenter is lower than 50 g / L, feed the fermenter, and the feed medium is the fermentation medium. After 300 hours of continuous operation, the permeation flux of the membrane is 400 g / m 2 / h, the separation facto...

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Abstract

The invention discloses an ethanol fermenting and separating coupling method using molasses as raw material, which aims at solving the problem of product inhibition in the existing biological ethanol production process. The ethanol fermenting and separating coupling method is characterized in that the fixed wine brewing yeast fermenting in-situ molasses pretreatment process is coupled with PDMS (polydimethylsiloxane) / ceramic membrane separating technique, and a complete molasses ethanol fermenting and separating coupling technology is established. The ethanol fermenting and separating coupling method has the advantages that the pollution to the PDMS / ceramic membrane in the running process is decreased, the inhibition action of product to the wine brewing yeast strain is reduced, and the output of ethanol is increased.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a separation and coupling technology of molasses ethanol fermentation. Background technique [0002] As an important industrial raw material, ethanol is widely used in food, chemical industry, medicine, dyestuff, national defense and other fields. At the same time, it is also a renewable clean energy, and its combustion value as a fuel reaches 26900 kJ / kg. [0003] Microbial fermentation is a commonly used ethanol production method at present, but free strains have poor tolerance to ethanol. Although immobilized cell fermentation can improve the strain tolerance and ethanol fermentation efficiency to a certain extent, the problem of product inhibition in the ethanol fermentation process still exists. When the ethanol concentration in the fermentation environment reached 6% (w / w), the growth and fermentation process of yeast cells were significantly inhibited;...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/06C12N11/10C12N11/04C07C29/76C07C31/08C12R1/865
CPCC07C29/76C12N11/04C12N11/10C12P7/06C07C31/08Y02E50/10
Inventor 陈可泉李梅何珣张阿磊齐雁斌魏国光欧阳平凯
Owner NANJING TECH UNIV
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