Immune cell preparation for treating chronic atrophic gastritis and gastric precancerous lesions and preparation method and application of immune cell preparation

A technology for atrophic gastritis and gastric precancerous lesions, applied in the field of biomedicine, can solve the problems of inability to achieve mass production and industrial development, inability to facilitate patient treatment, and limited cell sources, and to achieve mass production and production. Industrialization development, remarkable curative effect, and the effect of expanding the source

Inactive Publication Date: 2016-12-07
盛春华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of patients' autologous cell therapy, the source of cells is limited, the treatment of individuals is limited, the price is high, it is impossible to achieve mass production and industrial development, and it is impossible to make more

Method used

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  • Immune cell preparation for treating chronic atrophic gastritis and gastric precancerous lesions and preparation method and application of immune cell preparation
  • Immune cell preparation for treating chronic atrophic gastritis and gastric precancerous lesions and preparation method and application of immune cell preparation
  • Immune cell preparation for treating chronic atrophic gastritis and gastric precancerous lesions and preparation method and application of immune cell preparation

Examples

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Effect test

Embodiment 1

[0025] This embodiment provides an immune cell preparation for the treatment of chronic atrophic gastritis and gastric precancerous lesions, which is prepared by the following steps: use a blood bag to take the peripheral blood leukocytes of healthy people isolated from a blood bank, and use alcohol with a concentration of 75vt% Wipe the outer wall of the blood bag 2 times. Using Ficoll-hypaque lymphocyte separation medium, mononuclear cells were separated from human peripheral blood leukocytes in blood bags by density gradient centrifugation; in the operation of density gradient centrifugation, the centrifugation speed was controlled at 2000rpm / min, and the centrifugation time was 20min. After the separation, sterile physiological saline was added to the isolated mononuclear cells and washed twice by centrifugation at a speed of 2000 rpm / min for 20 minutes. After centrifugation and washing, add GT-T551 culture medium to the washed mononuclear cells to obtain a basic culture s...

Embodiment 2

[0028] This example provides an immune cell preparation for the treatment of chronic atrophic gastritis and gastric precancerous lesions, which is prepared by the following steps: take whole blood from a healthy person in a blood bag, and wipe the outer wall of the blood bag twice with 72vt% alcohol . Using Ficoll-hypaque lymphocyte separation medium, mononuclear cells were separated from human peripheral blood leukocytes in blood bags by density gradient centrifugation; in the operation of density gradient centrifugation, the centrifugation speed was controlled at 1800rpm / min, and the centrifugation time was 22min. After the separation, sterile physiological saline was added to the isolated mononuclear cells and washed twice by centrifugation at a speed of 1800 rpm / min for 22 minutes. After centrifugation and washing, add GT-T551 culture medium to the washed mononuclear cells to obtain a basic culture system, and control the cell density in the basic culture system to 3.5×10 ...

Embodiment 3

[0030] This embodiment provides an immune cell preparation for the treatment of chronic atrophic gastritis and gastric precancerous lesions, which is prepared by the following steps: use a blood bag to take whole blood from a healthy person in a blood bank and separate red blood cell suspension, plasma, platelets and other components After leaving the remaining peripheral blood leukocytes, wipe the outer wall of the blood bag 3 times with 74vt% alcohol. Using Ficoll-hypaque lymphocyte separation medium, mononuclear cells were separated from human peripheral blood leukocytes in blood bags by density gradient centrifugation; in the operation of density gradient centrifugation, the centrifugation speed was controlled at 2200rpm / min, and the centrifugation time was 18min. After the separation, sterile physiological saline was added to the isolated mononuclear cells and washed by centrifugation for 3 times at a rotational speed of 2200 rpm / min for 18 minutes. After centrifugation a...

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PUM

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Abstract

The invention belongs to the technical field of biological medicines and provides an immune cell preparation for treating chronic atrophic gastritis and gastric precancerous lesions. The immune cell preparation is prepared through the steps that peripheral blood of the human is collected, mononuclear cells are isolated, the isolated mononuclear cells are cultured to induce amplification, and after culture is completed, the immune cell preparation for treating chronic atrophic gastritis and gastric precancerous lesions is obtained. The invention further provides application of the immune cell preparation to preparation of medicines for treating chronic atrophic gastritis and/or gastric precancerous lesions and medicines for treating gastrointestinal reaction caused by chemotherapy. The immune cell preparation can improve the digestive tract symptoms of a patient, the curative effects of the immune cell preparation are remarkable on the aspects of treating chronic atrophic gastritis and reversing gastric precancerous lesions, both symptoms and root causes are treated, the curative effect is good, and relapse is not prone to occurring. By means of a preparation method, the stable immune cell preparation can be efficiently acquired, leukocyte generally abandoned by a blood station can serve as a cell source, waste is turned into wealth, and the cell immune preparation is suitable for being industrially produced on a large scale.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to an immune cell preparation for treating chronic atrophic gastritis and gastric precancerous lesions, a preparation method and application thereof. Background technique [0002] Gastric cancer is a common malignant tumor that endangers human health. In my country, more than 260,000 patients die of gastric cancer every year. Gastric precancerous lesions are the initial stage of gastric cancer, and the annual rate of cancer progression to invasive cancer is as high as 73%. In recent years, due to factors such as environment, diet, work pressure and Helicobacter pylori infection, the incidence of gastric precancerous lesions has increased significantly. Therefore, early blocking of the occurrence and development of gastric precancerous lesions has positive significance for the prevention of gastric cancer. [0003] Chronic atrophic gastritis is an important manifestat...

Claims

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Application Information

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IPC IPC(8): A61K35/17A61K35/15A61P1/00A61P1/04A61P35/00C12N5/078
CPCA61K35/17A61K35/15C12N5/0634C12N2500/84C12N2501/2301C12N2501/2302C12N2501/2304C12N2501/24C12N2501/998
Inventor 盛春华
Owner 盛春华
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