Preparation method of chitosan/mesoporous molecular sieve immobilized laccase and degradation method of phenolic wastewater

A technology of immobilizing laccase and molecular sieve, which is applied in the field of environmental biotechnology and engineering, can solve the problems that hinder the wide application of laccase, high cost of use, and low catalytic efficiency, and achieve high activity recovery rate, enhanced mechanical strength, and enhanced degradation speed effect

Active Publication Date: 2016-12-07
湖南海正生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The research on laccase has attracted more and more attention from environmental microbiologists, but there are still some problems, such as low catalytic efficiency and high cost of use, which hinder the wide application of laccase in the water treatment industry.

Method used

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  • Preparation method of chitosan/mesoporous molecular sieve immobilized laccase and degradation method of phenolic wastewater
  • Preparation method of chitosan/mesoporous molecular sieve immobilized laccase and degradation method of phenolic wastewater

Examples

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Effect test

Embodiment 1

[0036] A preparation method of chitosan / micro-mesozeol immobilized laccase, comprising the following steps:

[0037] (1) Get 5.0g commercial ZSM-5 zeolite (silicon-aluminum ratio (SiO2 / Al 2 o 3 )=18-30; relative crystallinity: Na type>85%; pore size: ), placed in 250mL of 0.4mol / L NaOH solution, stirred at room temperature for 40min; transferred to a vacuum drying oven, treated at 50°C for 3h, filtered through a 0.45um membrane filter, washed with deionized water, and placed at 110°C Dry in a vacuum oven for 12 hours to obtain a modified ZSM-5 molecular sieve;

[0038] (2) Take 5.0g of modified ZSM-5 molecular sieve and 5mL of 820U / mL laccase crude enzyme solution and stir at 25°C for 10h, centrifuge at 4000r / min for 5min to separate; use citric acid-phosphoric acid with a pH of 4.5 The precipitate was washed with disodium hydrogen buffer solution, and 1 mL of the supernatant was added to a 1.0 mmol / L guaiacol solution until no enzyme activity was detected to obtain Lac@Z...

Embodiment 2

[0065] A preparation method of chitosan / micro-mesozeol immobilized laccase, comprising the following steps:

[0066] (1) 10.0g commercial ZSM-5 zeolite (silicon to aluminum ratio (SiO 2 / Al 2 o 3 )=18-30; relative crystallinity: Na type>85%; pore size: ), placed in 600mL of 0.5mol / L NaOH solution, stirred at room temperature for 40min; transferred to a vacuum drying oven, treated at 50°C for 4h, filtered through a 0.45um membrane filter, washed with deionized water, and placed at 110°C Dry in a vacuum oven for 12 hours to obtain a modified ZSM-5 molecular sieve;

[0067] (2) Take 5.0g of modified ZSM-5 molecular sieve and 10mL of 930U / mL laccase crude enzyme solution and stir at 25°C for 12h, centrifuge at 4000r / min for 7min to separate; use citric acid-phosphoric acid with a pH of 4.5 The precipitate was washed with disodium hydrogen buffer solution, and 1 mL of the supernatant was added to a 1.0 mmol / L guaiacol solution until no enzyme activity was detected to obtain a ...

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Abstract

The invention provides a preparation method of chitosan / mesoporous molecular sieve immobilized laccase and a degradation method of phenolic wastewater. Compared with the prior art, a ZSM-5 molecular sieve with double mesoporous is used as a carrier, laccase is immobilized through physical adsorption, the ZSM-5 molecular sieve of the immobilized laccase is wrapped by chitosan, immobilized pellets are prepared, and the defects that the molecular sieve is unstable and prone to disengagement for laccase loading while good in laccase adsorption performance, and laccase immobilization area is small and immobilization efficiency is low while chitosan has good biocompatibility and adaptabilityare overcome.

Description

technical field [0001] The invention belongs to the field of environmental biotechnology and engineering, and in particular relates to a preparation method of chitosan / micro-mesozeol immobilized laccase and a degradation method of phenol-containing wastewater. Background technique [0002] Laccases are a class of copper-containing polyphenol oxidases that use molecular oxygen as an electron acceptor to reduce it to water. Laccase has a wide range of substrates and can catalyze and oxidize a variety of refractory organic pollutants in water. An important application in industry is the removal of phenols, in addition to phenolic derivatives, aromatic amines and their derivatives, carboxylic acid And its derivatives, hormones, biochromes and polycyclic aromatic hydrocarbons, etc. [0003] The study of laccase has attracted more and more attention from environmental microbiologists, but there are still some problems, such as low catalytic efficiency and high cost of use, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N9/02C02F3/34C02F101/34
CPCC02F3/342C02F2101/345C12N9/0061C12N11/10C12Y110/03002
Inventor 唐海魏彬刘方舟徐建平
Owner 湖南海正生物科技有限公司
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