Preparation method of instant silverfish protein powder
A technology of protein powder and whitebait, which is applied in the field of preparation of instant whitebait protein powder, can solve the problems of restricting the development of whitebait industry and the shortage of whitebait, and achieve the advantages of consolidating the breeding industry, no fishy smell, and short enzymatic hydrolysis time Effect
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Embodiment 1
[0022] A kind of preparation method of instant whitebait protein powder, concrete steps are as follows:
[0023] (1) Raw material pretreatment: soak the fresh whitebait in normal saline for 0.4h, then wash with water for 3-4 times;
[0024] (2) Grinding: using a meat grinder to grind the whitebait to obtain minced whitebait, which is set aside;
[0025] (3) Enzymolysis: Add water and protease to the whitebait minced meat for enzymolysis; wherein, the protease is alkaline protease, and the amount of alkaline protease is that the ratio of alkaline protease to substrate is 650-8000u / g; enzymolysis temperature 45-65°C; enzymolysis time is 30-180min; enzymolysis pH is 7.0-10.0; the amount of water added is to control the mass concentration of whitebait minced meat to 2.50-30.00%;
[0026] (4) Enzyme inactivation: inactivate the hydrolyzate after enzymolysis in a water bath at 85°C for 25 minutes, then cool to 40°C, centrifuge to take the supernatant for later use, wherein the cent...
Embodiment 2
[0031] A kind of preparation method of instant whitebait protein powder, concrete steps are as follows:
[0032] (1) Raw material pretreatment: soak fresh whitebait in normal saline for 0.5h, and then wash with water for 3-4 times;
[0033] (2) Grinding: using a meat grinder to grind the whitebait to obtain minced whitebait, which is set aside;
[0034] (3) Enzymolysis: Add water and protease to the whitebait minced meat for enzymolysis; wherein, the protease is alkaline protease, and the amount of alkaline protease is that the ratio of alkaline protease to substrate is 650-8000u / g; enzymolysis temperature 45-65°C; enzymolysis time is 30-180min; enzymolysis pH is 7.0-10.0; the amount of water added is to control the mass concentration of whitebait minced meat to 2.50-30.00%;
[0035] (4) Enzyme inactivation: inactivate the hydrolyzate after enzymolysis in a 90°C water bath for 30 minutes, then cool to 45°C, centrifuge to take the supernatant for later use, wherein the centrif...
Embodiment 3
[0041] A kind of preparation method of instant whitebait protein powder, concrete steps are as follows:
[0042] (1) Raw material pretreatment: soak fresh whitebait in physiological saline for 0.6h, and then wash with water for 3-4 times;
[0043] (2) Grinding: using a meat grinder to grind the whitebait to obtain minced whitebait, which is set aside;
[0044] (3) Enzymolysis: Add water and protease to the whitebait minced meat for enzymolysis; wherein, the protease is alkaline protease, and the amount of alkaline protease is that the ratio of alkaline protease to substrate is 650-8000u / g; enzymolysis temperature 45-65°C; enzymolysis time is 30-180min; enzymolysis pH is 7.0-10.0; the amount of water added is to control the mass concentration of whitebait minced meat to 2.50-30.00%;
[0045] (4) Enzyme inactivation: inactivate the hydrolyzate after enzymolysis in a 95°C water bath for 35 minutes, then cool to 50°C, centrifuge to take the supernatant for later use, wherein the ...
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