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Lactasin preparation method and application

A technology of lactase production and culture medium, which is applied in the direction of pharmaceutical formula, medical preparations containing active ingredients, digestive system, etc., can solve the problems of low number of viable bacteria and weak vitality, achieve high number of viable bacteria and promote growth The effect of reproduction and strong vitality

Inactive Publication Date: 2016-12-21
华夏国药(菏泽)制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many defects in the traditional preparation method of raw milk enzymes, such as the small number of viable bacteria, weak vitality, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation method and application of lactase are characterized in that the preparation method comprises the following steps:

[0034] (1) strain purification

[0035] Take the freeze-dried strains of live Enterococcus, put them into the liquid medium at 37°C for 24 hours, then insert them into the plate medium and culture them at a constant temperature of 37°C for 48 hours, then insert them into a test tube of skimmed fresh milk and culture them at a constant temperature of 37°C for 12 hours , put the milk test tube under the ultraviolet light for 15 minutes, then insert it into the plate culture medium and incubate it at 37°C for 48 hours, then branch it into the test tube and set aside;

[0036] (2) Configure tertiary medium: configure primary, secondary, and tertiary medium;

[0037] (3) Sterilization of the culture medium: the sterilization time of the first, second and third grade culture medium is sterilized at 121°C for 30 minutes;

[0038] (4) Vaccination...

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PUM

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Abstract

The invention relates to an oral preparation containing active living enterostreptococcus component, in particular to a lactasin preparation method and application. The preparation method consists of strain purification, medium configuration, sterilization, inoculation, fermentation, thallus separation and other steps. According to the preparation method provided by the invention, the process is simple and easy to operate, the viable count is high, the vitality is strong, per gram of bacterial powder contains about 200 million of lactic acid bacteria, and vitality research finds that within a validity period of 2 years, the dead number of the bacteria is less than 5%. The product prepared by the method provided by the invention has stable quality, is safe and reliable, and has significant effects on treatment of children and adult gastrointestinal diseases like dyspepsia, abdominal distension, and diarrhea, etc.

Description

technical field [0001] The invention relates to an oral preparation containing active living Streptococcus enterica components, in particular to a preparation method and application of lactase. Background technique [0002] Lactase Sheng, also known as "Biaofeiming", is a traditional livebactin preparation. Lactase is produced as white or light yellow dry powder; slightly odorous, tasteless, almost insoluble in water. For indigestion, abnormal intestinal fermentation, diarrhea and green stools in children, etc. Lactase is a dry preparation of live enterococci, which decomposes sugars in the intestines to generate lactic acid, which increases the acidity in the intestines, thereby inhibiting the growth and reproduction of spoilage bacteria, preventing intestinal fermentation, and reducing gas production, thus promoting digestion and stopping. laxative effect. There are many defects in the traditional preparation method of lactase, for example, the number of live bacteria i...

Claims

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Application Information

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IPC IPC(8): A61K35/744A61P1/14A61P1/12A61P1/00
CPCA61K35/744
Inventor 陈长印马朝霞王星星
Owner 华夏国药(菏泽)制药有限公司
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