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Controllable preparation method of porphyrin/SiO2 co-assembly nano composite material for photodynamic therapy

A nano-composite material and photodynamic therapy technology, applied in the field of material chemistry, can solve the problems of low loading efficiency and cumbersome steps, achieve low equipment requirements, simple process, and solve the effects of porphyrin loading and output

Active Publication Date: 2017-01-04
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mesoporous SiO 2 Nanoparticles as carriers require the synthesis of mesoporous SiO 2 , and then the photosensitizer is physically adsorbed or chemically attached to the surface or pores of the medium, so the steps are cumbersome and the loading efficiency is very low
Therefore, how to improve the water solubility and biocompatibility of porphyrin and its derivatives while improving their loading efficiency is still a challenge for the application of porphyrin and its derivatives in biophototherapy.

Method used

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  • Controllable preparation method of porphyrin/SiO2 co-assembly nano composite material for photodynamic therapy
  • Controllable preparation method of porphyrin/SiO2 co-assembly nano composite material for photodynamic therapy
  • Controllable preparation method of porphyrin/SiO2 co-assembly nano composite material for photodynamic therapy

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Experimental program
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Embodiment 1

[0030] A porphyrin / SiO2 for photodynamic therapy 2 A method for the controllable preparation of co-assembled nanocomposites, comprising the following steps:

[0031] (1) Prepare a mixed solution of tetrapyridyl zinc porphyrin and hydrochloric acid, so that the concentration of tetrapyridyl zinc porphyrin in the mixed solution is 0.01M, and the concentration of hydrochloric acid is 0.2M;

[0032] (2) Prepare 0.01 M MTAB aqueous solution,

[0033] (3) Prepare tetraethyl orthosilicate methanol solution with a volume fraction of 20%;

[0034] (4) Under stirring, quickly inject 0.5 mL of the solution obtained in step (1) into 9.5 mL of the solution obtained in step (2) containing 96 μL of 1N sodium hydroxide standard solution. At this time, the pH of the mixed solution is measured to be 4.1;

[0035] (5) Manually add 20 μL of the solution obtained in step (3) to the solution obtained in step (4) every 15 minutes for a duration of 3.75 hours, and the total amount added is 300 μL; ...

Embodiment 2

[0039] A porphyrin / SiO2 for photodynamic therapy 2The controllable preparation method of co-assembled nanocomposites is different from Example 1 in that 98 μL of NaOH solution is added in step (4), and the pH of the solution is finally measured to be 5.3. The obtained material is as follows figure 2 The TEM image of A, showing a long square with a length of 106 nm and a width of 53 nm; figure 2 B Soak the material obtained in Example 2 with 1 M HCl solution for 5-10 s, centrifuge, and wash the precipitate twice with water. The TEM image of the obtained material shows no obvious change before and after soaking and still shows doped rectangles.

Embodiment 3

[0041] A porphyrin / SiO2 for photodynamic therapy 2 The controllable preparation method of co-assembled nanocomposites differs from Example 1 in that the amount of NaOH solution is regulated by 97 μL and 97.5 μL in step (4), and the pH of the solution is finally measured to be 4.4 and 4.7 respectively, and the obtained materials After soaking in 1 M HCl solution for 5-10 s, centrifuge and wash the precipitated material once with water.

[0042] The ultraviolet-visible absorption spectrum of embodiment 1,2,3 is as Figure 4 As shown, when the pH is lower, at 4.1 porphyrin / SiO 2 The composite material has peaks at 411nm and 452 nm, while Q-band absorption has absorption at 570 nm and 611 nm. As the pH value gradually increases, the peaks at 411 nm and 452 nm gradually blue-shift and become the peaks at 425 nm. A single peak, and the Q band gradually blue-shifted to 565 nm and 605 nm. Show that along with the pH value increases gradually, increases to 5.3 by 4.1, the existence ...

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Abstract

The invention relates to a controllable preparation method of a porphyrin / SiO2 co-assembly nano composite material for photodynamic therapy. The method comprises the following steps: (1) preparing a mixed solution of tetrapyridyl zinc porphyrin and hydrochloric acid, an MTAB aqueous solution and a methanol tetraethyl orthosilicate solution; (2), adding the mixed solution of the tetrapyridyl zinc porphyrin and HCl into an MTAB aqueous solution containing a NaOH solution under a stirring condition, wherein the pH of the mixed solution is 4.0 to 5.3; (3) dropwise adding the methanol tetraethyl orthosilicate solution into the solution in the step (2) every 10 to 20 minutes, and adding all methanol tetraethyl orthosilicate solution within 3 to 4 hours; and (4) stirring a reaction solution obtained in the step (3) for 40 to 50 h at a room temperature, centrifugally separating, and obtaining precipitates, i.e. the porphyrin / SiO2 co-assembly nano composite material. After the targeted modification by folic acid, the prepared composite material has an effect for accumulating rapidly-proliferated cells in priority, has excellent fluorescent imaging positioning capacity, has high photo therapy efficiency and the like.

Description

technical field [0001] The invention belongs to the field of material chemistry, and in particular relates to a porphyrin / SiO used in photodynamic therapy 2 A method for the controllable preparation of co-assembled nanocomposites. Background technique [0002] In recent years, as the incidence of cancer has been increasing year by year, its treatment methods have become increasingly diversified and efficient, from traditional surgical treatment, radiotherapy and chemotherapy to hyperthermia, phototherapy combined with chemotherapy and radiotherapy. Modal combination therapy, such a transformation greatly reduces the disadvantages of traditional treatment, such as easy recurrence after surgery, systemic blind surgery, and large local side effects. Capacitance, excellent cancer cell targeting, and high cancer cell killing efficiency have attracted more and more attention. [0003] Photodynamic therapy (PDT) refers to a photosensitive reaction accompanied by biological ef...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K9/14B82Y5/00A61P35/00
CPCA61K9/143A61K41/0071B82Y5/00
Inventor 白锋王杰菲南楠钟永王亮毛兴东
Owner HENAN UNIVERSITY
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