Method for preparing microalgae biodiesel

A technology of microalgae and biodiesel, which is applied in the fields of biofuel, petroleum industry, and fat production. It can solve the problems of low biodiesel production, low catalytic efficiency, and long reaction time, and achieve strong environmental adaptability and improved catalytic efficiency. , the effect of large reaction interface

Inactive Publication Date: 2017-01-04
南宁华侨投资区政孙贸易有限公司
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing biodiesel production process generally has the following problems: low catalytic efficiency, low...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of Botrytis branii culture medium: Glucose 25mg / L, NaNO 3 1.8g / L, KH 2 PO 4 1.0mg / L, chitosan 1.2mg / L, biogas slurry 8mg / L, f2 vitamin 4mL and seawater 1L;

[0027] A method for preparing microalgae biodiesel, comprising the following steps:

[0028] S1. Cultivation of Botrytis braziliana: use Botrytis brawny as raw material, inoculate it into the culture medium at an inoculation density of 4 million cells / mL, and irradiate it with red and blue LED supplementary light for 10h / day, and the red light in the LED supplementary light The ratio with blue light is 1:1, the light intensity is controlled at 4500LX, the culture temperature is controlled at 25°C, cultured for 7 days, the density of Botrytis brachyphylla reaches 60 million cells / mL, and the Botrytis brachyphylla cells are collected;

[0029] S2. Grease extraction: After concentrating and drying the staphylococcal algae cells collected in step S1, use ultrasonic waves to break the wall of staphylococ...

Embodiment 2

[0034] Preparation of Botrytis branii culture medium: Glucose 20mg / L, NaNO 3 2.2g / L, KH 2 PO 4 1.1mg / L, chitosan 1.0mg / L, biogas slurry 7mg / L, f2 vitamin 2mL and seawater 1L;

[0035] A method for preparing microalgae biodiesel, comprising the following steps:

[0036]S1. Cultivation of Botrytis braziliana: use Botrytis brawny as raw material, inoculate it into the culture medium at an inoculation density of 3 million cells / mL, and irradiate it with red and blue LED supplementary light for 12h / day, and the red light in the LED supplementary light The ratio with blue light is 2:1, the light intensity is controlled at 4200LX, the culture temperature is controlled at 24°C, cultured for 7 days, the density of Botrytis brachyphylla reaches 60 million cells / mL, and the cells of Botrytis brachyphylla are collected;

[0037] S2. Grease extraction: After concentrating and drying the staphylococcal algae cells collected in step S1, use ultrasonic waves to break the wall of staphyloc...

Embodiment 3

[0042] Preparation of Botrytis branii culture medium: Glucose 30mg / L, NaNO 3 2.4g / L, KH 2 PO 4 1.3mg / L, chitosan 0.8mg / L, biogas slurry 9mg / L, f2 vitamin 3mL and seawater 1L;

[0043] A method for preparing microalgae biodiesel, comprising the following steps:

[0044] S1. Cultivation of Botrytis braziliana: use Botrytis brawny as raw material, inoculate it into the culture medium at an inoculation density of 5 million cells / mL, and irradiate it with red and blue LED supplementary light for 14h / day, and the red light in the LED supplementary light The ratio with blue light is 3:1, the light intensity is controlled at 4000LX, the culture temperature is controlled at 23°C, cultured for 8 days, the density of Staphylococcus branici reaches 60 million cells / mL, and the cells of Staphylococcus branici are collected;

[0045] S2. Grease extraction: After concentrating and drying the staphylococcal algae cells collected in step S1, use ultrasonic waves to break the wall of staphy...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing microalgae biodiesel. The method includes the following steps that botryococcus braunii is inoculated to a culture medium and is irradiated by using a red and blue double-color LED light supplementing lamp for 10-14 hours per day, culture is performed at the temperature of 23-25 DEG C for 7-8 days, and then botryococcus braunii cells can be collected; the collected botryococcus braunii cells are subjected to wall-breaking treatment by ultrasonic waves, extraction is performed by using an organic solvent to obtain microalgae oil, then the microalgae oil, methanol and a zeolite molecular sieve catalyst are added to a rector according to the weight ratio of 1 to 4-6 to 0.02-0.04, esterification reaction is performed to obtain an esterification product, and the esterification product is subjected to separation and purification to obtain the biodiesel. According to the method, the botryococcus braunii short in production cycle, widely distributed, strong in photosynthesis and high in oil content is used as the raw material, and the problems existing in plant oil raw materials for biodiesel development are solved. In addition, the method also has the advantages of being simple in preparation process, high in catalytic efficiency, high in oil extraction rate, high in product yield and the like, and a good foundation is laid for large-scale biodiesel industrialization.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, in particular to a method for preparing microalgae biodiesel. Background technique [0002] With the increasing depletion of fossil fuels and the sharp rise in oil prices, the search for renewable energy has become a solution to human survival and to maintain the sustainable development of the world economy. As a new alternative energy, biodiesel is developing rapidly in countries all over the world. Biodiesel refers to oil crops such as soybeans, rapeseed, cotton, palm, etc., wild oil plants and engineering microalgae and other aquatic vegetable oils, animal fats, and catering waste oils, etc. Renewable diesel fuel to replace petrodiesel. The advantages of biodiesel are not limited to renewability. Compared with existing diesel, it also has the following advantages: high cetane number, which makes the combustion performance better; better wetting performance, which makes the fuel injecti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C11B1/02C11B1/04C11B1/10C11B3/12C11C3/10C10L1/02
CPCC10L1/02C11B1/025C11B1/04C11B1/10C11B3/12C11C3/10Y02E50/10
Inventor 黄健峻其他发明人请求不公开姓名
Owner 南宁华侨投资区政孙贸易有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap