Microorganism-loaded sandwiched micro-nano fiber composite membrane and preparation method and application thereof

A technology of micro-nano fibers and microorganisms, applied in the field of new materials, to achieve the effect of low raw material cost, simple operation process and wide range of adsorption concentration

Active Publication Date: 2017-01-04
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although there are many researches and patents related to immobilized microorganisms, there is still no electrostatic spraying of raw materials on the nanofiber membranes prepared by electrospinning as in the present invention, and the process of alternating electrospinning-electrostatic spraying to obtain microorganism-loaded An example of a sandwich micro-nano fiber composite membrane. In addition, adding it to the reaction system has the advantages of easy operation, non-toxic, harmless, reusable, and low cost.

Method used

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  • Microorganism-loaded sandwiched micro-nano fiber composite membrane and preparation method and application thereof
  • Microorganism-loaded sandwiched micro-nano fiber composite membrane and preparation method and application thereof
  • Microorganism-loaded sandwiched micro-nano fiber composite membrane and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) First, weigh 7.56 g and 2.52 g each of formic acid and acetone at a mass ratio of 3:1, mix them evenly, then add polycaprolactone and chitosan into the formic acid-acetone mixed solvent, and at room temperature Magnetic stirring for 5 h. After the polycaprolactone and chitosan are dissolved, add rectorite and continue magnetic stirring at room temperature for 20 hours to make rectorite dispersed evenly to obtain an electrostatic spinning solution; in the electrostatic spinning solution, the polycaprolactone is The mass fraction is 9%, and the mass fraction of chitosan is 2%. The mass of rectorite is 0.5% of the total mass of polycaprolactone and chitosan.

[0047] (2) After the electrospinning solution is stirred, put it into the syringe, turn on the high-voltage power supply, and adjust it to a predetermined voltage. Then the polycaprolactone micro-nano fiber membrane is prepared by electrospinning technology. The parameters include: the perfusion speed is 1.5 mL / h,...

Embodiment 2

[0051] (1) First, weigh 1.04 g of polycaprolactone and 0.23 g of chitosan, and then add them to a mixed solvent of formic acid and acetone. The mass of formic acid is 7.56 g and the mass of acetone is 2.52 g. And magnetically stirred at room temperature for 5 hours, after the polycaprolactone and chitosan are dissolved, add rectorite, the mass of rectorite is 0.5% of the total mass of polycaprolactone and chitosan, continue at room temperature Stir under magnetic force for 20 h to disperse the rectorite evenly and obtain an electrostatic spinning solution.

[0052] (2) After the electrostatic spinning solution is evenly stirred, put it into the syringe, turn on the high-voltage power supply, and adjust it to a predetermined voltage. Then the polycaprolactone micro-nano fiber membrane is prepared by electrospinning technology. The parameters include: the perfusion speed is 1.5 mL / h, the voltage of the high-voltage DC power supply is 10 kV, the distance between the needle and the r...

Embodiment 3

[0058] (1) First, weigh 1.04 g of polycaprolactone and 0.23 g of chitosan, and then add them to a mixed solvent of formic acid and acetone. The mass of formic acid is 7.56 g and the mass of acetone is 2.52 g. And magnetically stirred at room temperature for 5 hours. After the polycaprolactone and chitosan are dissolved, add rectorite. The mass of rectorite is 0.5% of the total mass of polycaprolactone and chitosan. Continue at room temperature. Stir under magnetic force for 20 h to disperse the rectorite evenly and obtain an electrostatic spinning solution.

[0059] (2) After the electrostatic spinning solution is evenly stirred, put it into the syringe, turn on the high-voltage power supply, and adjust it to a predetermined voltage. Then the polycaprolactone micro-nano fiber membrane is prepared by electrospinning technology. The parameters include: the perfusion rate is 1.5mL / h, the voltage of the high-voltage DC power supply is 10 kV, the distance from the needle to the receiv...

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Abstract

The invention discloses a method for preparing a microorganism-loaded sandwiched micro-nano fiber composite membrane capable of adsorbing heavy metals on the basis of alternate use of electrostatic spinning and electrostatic spraying. The method comprises the steps that a polymer composite is dissolved into an organic solvent, and magnetic stirring is conducted to obtain a mixed solution; a micro-nano fiber membrane is prepared through an electrostatic spinning technology; microorganisms are dispersed into deionized water, and the solution is stirred to be uniform and electrostatically sprayed on the surface of the micro-nano fiber membrane; a sample is put into a vacuum drying oven to be dried till the solvent is completely volatilized, and a double layer is obtained; the microorganism-loaded sandwiched micro-nano fiber composite membranes with the different needed odd and even layer numbers can be obtained through repeated operation. What is more novel is that the material can achieve repeated desorption and reutilization and is significant in adsorption effect; in addition, the method is low in raw material cost, good in biocompatibility, mild in reaction condition, wide in applicability and the like and is expected to be applied to large-scale industrial production.

Description

Technical field [0001] The invention belongs to the field of new materials, and specifically relates to a sandwich micro-nano fiber composite membrane loaded with microorganisms prepared by alternately preparing electrostatic spinning-electrostatic spraying, a preparation method thereof, and an application thereof in the field of repairing heavy metal pollution. Background technique [0002] Since the 20th century, with the rapid development of science and technology, while promoting economic development and improving people's living standards, mankind has also paid a heavy price. For example, with the rapid development of industries such as metallurgy, mining, electroplating, pesticides and leather, papermaking and battery manufacturing, various sewage containing heavy metals is directly or indirectly discharged to the external environment. As the biological chain continues to accumulate, it exceeds the normal range. It directly threatens human health and leads to the deteriorat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/12C12N11/10C12N11/08C12N11/02C02F3/34D01D5/00D04H1/728C12R1/865C12R1/07C02F101/20C02F101/22
CPCC02F3/347C02F2101/20C02F2101/206C02F2101/22C12N11/02C12N11/08C12N11/10C12N11/12C12N11/14D01D5/003D01D5/0061D01D5/0092D04H1/728
Inventor 邓红兵邱晓丹曾召阳施晓文杜予民
Owner WUHAN UNIV
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