Sandwich micro-nano fiber composite membrane loaded with microorganisms and its preparation method and application

A micro-nano fiber and microorganism technology, which is applied in the field of repairing heavy metal pollution, sandwiched micro-nano fiber composite membrane and its preparation field, achieves the effects of wide adsorption concentration range, simple operation process and high recycling rate

Active Publication Date: 2019-08-02
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although there are many researches and patents related to immobilized microorganisms, there is still no electrostatic spraying of raw materials on the nanofiber membranes prepared by electrospinning as in the present invention, and the process of alternating electrospinning-electrostatic spraying to obtain microorganism-loaded An example of a sandwich micro-nano fiber composite membrane. In addition, adding it to the reaction system has the advantages of easy operation, non-toxic, harmless, reusable, and low cost.

Method used

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  • Sandwich micro-nano fiber composite membrane loaded with microorganisms and its preparation method and application
  • Sandwich micro-nano fiber composite membrane loaded with microorganisms and its preparation method and application
  • Sandwich micro-nano fiber composite membrane loaded with microorganisms and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) First, weigh 7.56 g and 2.52 g of formic acid and acetone respectively at a mass ratio of 3:1, mix well, then add polycaprolactone and chitosan to formic acid-acetone mixed solvent, under normal temperature conditions Stir magnetically for 5 h. After polycaprolactone and chitosan were dissolved, rectorite was added, and magnetic stirring was continued at room temperature for 20 h to disperse the rectorite evenly to obtain an electrospinning solution; in the electrospinning solution, polycaprolactone The mass fraction is 9%, and the mass fraction of chitosan is 2%. The mass of rectorite is 0.5% of the total mass of polycaprolactone and chitosan.

[0047] (2) After the electrospinning solution is stirred, put it into the syringe, turn on the high voltage power supply, and adjust to the predetermined voltage. Then, the polycaprolactone micro-nanofiber membrane was prepared by electrospinning technology, and the parameters included: the perfusion rate was 1.5 mL / h, th...

Embodiment 2

[0051] (1) First, weigh 1.04 g of polycaprolactone and 0.23 g of chitosan, respectively, and then add them into a mixed solvent of formic acid and acetone. The mass of formic acid is 7.56 g, and the mass of acetone is 2.52 g. And magnetically stirred at room temperature for 5 h. After polycaprolactone and chitosan were dissolved, rectorite was added. The quality of rectorite was 0.5% of the total mass of polycaprolactone and chitosan. Under magnetic stirring for 20 h, the rectorite was uniformly dispersed to obtain an electrospinning solution.

[0052] (2) After the electrospinning solution is stirred evenly, put it into the syringe, turn on the high voltage power supply, and adjust to the predetermined voltage. Then, the polycaprolactone micro-nanofiber membrane was prepared by electrospinning technology, and the parameters included: the perfusion rate was 1.5 mL / h, the voltage of the high-voltage DC power supply was 10 kV, the distance from the needle to the receiving plate ...

Embodiment 3

[0058] (1) First, weigh 1.04 g of polycaprolactone and 0.23 g of chitosan, respectively, and then add them into a mixed solvent of formic acid and acetone. The mass of formic acid is 7.56 g, and the mass of acetone is 2.52 g. And under normal temperature condition magnetic stirring 5h, after polycaprolactone and chitosan dissolve, add rectorite again, the quality of rectorite is 0.5% of polycaprolactone and chitosan total mass, continue Magnetic stirring was performed for 20 h to disperse the rectorite evenly to obtain an electrospinning solution.

[0059] (2) After the electrospinning solution is stirred evenly, put it into the syringe, turn on the high voltage power supply, and adjust to the predetermined voltage. Then, the polycaprolactone micro-nanofiber membrane was prepared by electrospinning technology. The parameters included: the perfusion rate was 1.5mL / h, the voltage of the high-voltage DC power supply was 10 kV, the distance from the needle to the receiving plate was...

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Abstract

The invention discloses a method for preparing a microorganism-loaded sandwiched micro-nano fiber composite membrane capable of adsorbing heavy metals on the basis of alternate use of electrostatic spinning and electrostatic spraying. The method comprises the steps that a polymer composite is dissolved into an organic solvent, and magnetic stirring is conducted to obtain a mixed solution; a micro-nano fiber membrane is prepared through an electrostatic spinning technology; microorganisms are dispersed into deionized water, and the solution is stirred to be uniform and electrostatically sprayed on the surface of the micro-nano fiber membrane; a sample is put into a vacuum drying oven to be dried till the solvent is completely volatilized, and a double layer is obtained; the microorganism-loaded sandwiched micro-nano fiber composite membranes with the different needed odd and even layer numbers can be obtained through repeated operation. What is more novel is that the material can achieve repeated desorption and reutilization and is significant in adsorption effect; in addition, the method is low in raw material cost, good in biocompatibility, mild in reaction condition, wide in applicability and the like and is expected to be applied to large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of new materials, and specifically relates to a sandwich micro-nano fiber composite membrane loaded with microorganisms prepared alternately by electrospinning and electrostatic spraying, a preparation method thereof, and an application thereof in the field of repairing heavy metal pollution. Background technique [0002] Since the 20th century, with the rapid development of science and technology, while promoting economic development and improving people's living standards, human beings have also paid a heavy price. For example, with the rapid development of industries such as metallurgy, mining, electroplating, pesticides and leather, papermaking and battery manufacturing, various sewage containing heavy metals are directly or indirectly discharged to the external environment. With the continuous enrichment of the biological chain, it exceeds the normal range. It directly threatens human health and leads to the dete...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/12C12N11/10C12N11/08C12N11/02C02F3/34D01D5/00D04H1/728C12R1/865C12R1/07C02F101/20C02F101/22
CPCC02F3/347C02F2101/20C02F2101/206C02F2101/22C12N11/02C12N11/08C12N11/10C12N11/12C12N11/14D01D5/003D01D5/0061D01D5/0092D04H1/728
Inventor 邓红兵邱晓丹曾召阳施晓文杜予民
Owner WUHAN UNIV
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