Microbial agent for treating nickel pollution in tropical acidic soil and preparing method thereof

Abstract

The invention discloses a microbial agent for treating nickel pollution in tropical acidic soil and preparing method thereof, wherein the agent comprises a preservative solution and a nickel-resistant eosinophilic heterotrophic strain preserved in the preservative solution; the preparing method comprises the following four steps: inoculation of microorganisms, screening and purification of target microorganisms, cultivation of target microorganisms, preparing and preservation of target microbial agents. The screening and purification of target microorganism needs to be screened and purified by using the solid MRS medium supplemented with Ni2 +. The culture target Microorganisms were cultured on standard solid MRS medium. The final preparings are cultivated in standard liquid MRS medium. The preservation method is to inject the liquid paraffin after sterilization in the liquid MRS medium. The microbial agent can adapt to the acidic environment, propagate naturally, continuously expand the scope of treatment and adapt to the high nickel environment with thorough governance degree.

Description

technical field [0001] The invention relates to the field of heavy metal prevention and control, in particular to a microbial preparation for treating nickel pollution in tropical acidic soil and a manufacturing method thereof. Background technique [0002] Nickel pollution is environmental pollution caused by nickel and its compounds. The average abundance of nickel in the earth's crust is 75ppm, which is relatively abundant in trace elements. It has a strong sulfide affinity and exists mainly in the form of nickel sulfide ore and nickel oxide ore. In iron, cobalt, copper and some rare earth ores, nickel often coexists. The annual migration of nickel in the world is as follows: 320,000 tons of rock weathering, 19,000 tons of river transport, 560,000 tons of mining, and 5,600 tons of fossil fuel combustion emissions. Nickel is currently considered a potential hazard to the environment. Most of the nickel in the industry is used to make stainless steel and anti-corrosion a...

AI Technical Summary

Problems solved by technology

[0003] Among the relevant patents that have been applied in China, the patent "A Repair Method for Heavy Metal Nickel Contaminated Soil" (Application No.: 201510213271.X, Publication Date: 2015-08-19) discloses a method of rinsing with acetic acid solution before using Electrochemical recovery of nickel is a treatment method, but the treatment cost of this method is high (the mass ratio of acetic acid solution to soil reaches 1:1), and it cannot be repaired in situ, and the repair efficiency is low; the patent "Using Pseudomonas vesicularis to promote Phytoremediation of Nickel Polluted Soil" (Application No.: 200510034858.0, Publication Date: 2005-11-16) discloses a soil treatment method for nickel-contaminated soil that uses the joint action of Pseudomonas vesicularis bacteria liquid and hyperaccumulator plants method, but the super-enrichment plants used in this method are unknown, and the plants enriched in nickel are basically not economically beneficial, and the treatment time is very long (twice a year, but how long it can be treated is not given at all), and During the treatment process, normal plant cultivation cannot be carried out, which wastes land resources