Preparation method of ganglioside extract

A technology of ganglioside and extract is applied in the field of eluent passing through ion exchange column chromatography, which can solve the problems of wasteful cost, low amount of GM1, complicated technological process, etc. The effect of uniform heat conduction

Inactive Publication Date: 2017-01-25
丁海麦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In existing methods, GM is separated from many gangliosides by chromatographic methods 1 , the process is more complicated, plus the GM in the total gan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Take fresh pig brain, tear off the meninges and surface blood vessels, wash with water, and carry out microwave vacuum drying on the cleaned pig brain. , the power is 2Kw, the dry pig brain that obtains water content is 3.5wt%, the dried pig brain is pulverized into pig brain powder, and the average particle size of pig brain powder is 80~150nm;

[0038](2) Take 1 kg of pig brain powder, add 5.6L of absolute ethanol to the obtained pig brain powder, stir for 3.5 hours at a temperature of 38-43°C, and the stirring speed is 300 rpm, and then filter to obtain a precipitate I;

[0039] (3) Add acetic acid-sodium acetate buffer solution, isopropanol and dichloromethane mixed solvent of pH5.4 in precipitate I, the consumption of mixed solvent is 6 times of precipitate weight, wherein, buffer solution, isopropanol and methylene chloride at a volume ratio of 6.5:1.2:5.5, stirred at room temperature for 100 min, and centrifuged to obtain supernatant I;

[0040] (4) Cool th...

Embodiment 2

[0043] (1) Take fresh pig brain, tear off the meninges and surface blood vessels, wash with water, and carry out microwave vacuum drying on the cleaned pig brain. , the power is 2Kw, the dry pig brain that obtains water content is 2.9wt%, the dried pig brain is pulverized into pig brain powder, and the average particle size of pig brain powder is 80~150nm;

[0044] (2) Take 1 kg of pig brain powder, add 6.2L of absolute ethanol to the obtained pig brain powder, stir at a temperature of 45-50°C for 3.5 hours, and the stirring speed is 300 rpm, and then filter to obtain a precipitate I;

[0045] (3) Add acetic acid-sodium acetate buffer solution, isopropanol and dichloromethane mixed solvent of pH5.0 in precipitate I, the consumption of mixed solvent is 6 times of precipitate weight, wherein, buffer solution, isopropanol and methylene chloride at a volume ratio of 4.5:0.8:3.0, stirred at room temperature for 100 min, and centrifuged to obtain supernatant I;

[0046] (4) Cool t...

Embodiment 3

[0049] (1) Take fresh pig brain, tear off the meninges and surface blood vessels, wash with water, and carry out microwave vacuum drying on the cleaned pig brain. , to obtain dried pig brain with a water content of 4.0wt%, crushing the dried pig brain into pig brain powder, the average particle size of the pig brain powder is 80-150nm;

[0050] (2) Take 1 kg of pig brain powder, add 7.5L of absolute ethanol to the obtained pig brain powder, stir at a temperature of 35-40°C for 4.5 hours, and the stirring speed is 400 rpm, and then filter to obtain a precipitate I;

[0051] (3) Add acetic acid-sodium acetate buffer solution, isopropanol and dichloromethane mixed solvent of pH5.0 in precipitate I, the consumption of mixed solvent is 5 times of precipitate weight, wherein, buffer solution, isopropanol and dichloromethane at a volume ratio of 5.5:1.0:3.5, stirred at room temperature for 60 minutes, and centrifuged to obtain supernatant I;

[0052] (4) Cool the supernatant I to -...

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PUM

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Abstract

A preparation method of ganglioside extract includes subjecting fresh pig brains to microwave vacuum drying treatment to obtain dry pig brains with moisture content lower than 5wt%, and grinding the dry pig brains into pig brain powder, wherein the vacuum degree for microwave vacuum drying ranges from 0.04 Mpa to 0.09 Mpa; adding absolute ethyl alcohol into the dry pig brain powder, stirring for 2.0-5.0 hours at 35-55 DEG C, and filtering to obtain precipitate I; adding a mixed solvent of an acetic acid-sodium acetate buffer solution with a pH being 5.0-5.4, isopropanol and dichloromethane into the precipitate I, stirring for 60-120 minutes at a room temperature, and conducting centrifugal separation to obtain liquid supernatant I; cooling the liquid supernatant I to 15-25 DEG C to enable precipitate II to separate out, and filtering to obtain the precipitate II; repeatedly washing the precipitate II by anhydrous ether prior to filtration and vacuum drying so as to obtain the ganglioside extract. The preparation method of the ganglioside extract has the advantages that consumption of harmful solvents is reduced substantially, and the prepared ganglioside extract is high in ganglioside purity and GM1 content.

Description

Background technique [0001] Gangliosides, the most complex glycosphingolipids, are present in trace amounts in almost all tissues of vertebrates, but are most abundant in mammalian brain and spinal cord tissues. It has the function of enhancing the development of human brain function and enhancing memory. Ganglioside is also an information regulator in the nervous system. It has obvious curative effect and role in treatment and biological protection. The main gangliosides in mammalian brain and spinal cord tissues are GM1, GD1b, GD1a and GT1b. The physiological functions of ganglioside molecules mainly include: 1) as a component of membrane lipid bilayer and cell glycocalyx; 2) to maintain negative charge on the membrane surface; 3) to participate in cell recognition and signal transmission; 4) to protect against ischemia ( Oxygen-induced nerve damage, etc. [0002] The basic structure of biological membranes is a lipid bilayer. According to the different lipid skeleton stru...

Claims

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Application Information

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IPC IPC(8): C07H15/10C07H1/08A61K35/30A61P25/00
CPCA61K35/30C07H1/08C07H15/10
Inventor 丁海麦李彩艳
Owner 丁海麦
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