Preparation method of ganglioside extract

Abstract

A preparation method of ganglioside extract includes subjecting fresh pig brains to microwave vacuum drying treatment to obtain dry pig brains with moisture content lower than 5wt%, and grinding the dry pig brains into pig brain powder, wherein the vacuum degree for microwave vacuum drying ranges from 0.04 Mpa to 0.09 Mpa; adding absolute ethyl alcohol into the dry pig brain powder, stirring for 2.0-5.0 hours at 35-55 DEG C, and filtering to obtain precipitate I; adding a mixed solvent of an acetic acid-sodium acetate buffer solution with a pH being 5.0-5.4, isopropanol and dichloromethane into the precipitate I, stirring for 60-120 minutes at a room temperature, and conducting centrifugal separation to obtain liquid supernatant I; cooling the liquid supernatant I to 15-25 DEG C to enable precipitate II to separate out, and filtering to obtain the precipitate II; repeatedly washing the precipitate II by anhydrous ether prior to filtration and vacuum drying so as to obtain the ganglioside extract. The preparation method of the ganglioside extract has the advantages that consumption of harmful solvents is reduced substantially, and the prepared ganglioside extract is high in ganglioside purity and GM1 content.

Description

Background technique [0001] Gangliosides, the most complex glycosphingolipids, are present in trace amounts in almost all tissues of vertebrates, but are most abundant in mammalian brain and spinal cord tissues. It has the function of enhancing the development of human brain function and enhancing memory. Ganglioside is also an information regulator in the nervous system. It has obvious curative effect and role in treatment and biological protection. The main gangliosides in mammalian brain and spinal cord tissues are GM1, GD1b, GD1a and GT1b. The physiological functions of ganglioside molecules mainly include: 1) as a component of membrane lipid bilayer and cell glycocalyx; 2) to maintain negative charge on the membrane surface; 3) to participate in cell recognition and signal transmission; 4) to protect against ischemia ( Oxygen-induced nerve damage, etc. [0002] The basic structure of biological membranes is a lipid bilayer. According to the different lipid skeleton stru...

AI Technical Summary

Problems solved by technology

[0010] In existing methods, GM is separated from many gangliosides by chromatographic methods 1 , the process is more complicated, plus the GM in the total ganglioside lipid 1 The content is relatively low, then, the GM proposed by the complex process 1 The quantity is also relatively small, which is a waste of cost