Method for extracting chondroitin sulfate from sturgeon bones, chondroitin sulfate extracted through method, and application of chondroitin sulfate

Abstract

The invention relates to a chondroitin sulfate extraction method, and especially relates to a method for extracting chondroitin sulfate from sturgeon bones through a diluted alkali-enzymatic hydrolysis technology. The method is characterized in that alkali liquid extraction and salt precipitation are carried out to remove proteins, and the method concretely comprises the following steps: adding a NaOH solution with the concentration of 3% to dried sturgeon cartilages according to a solid-to-liquid ratio of 1:6 (g/ml), extracting at 35 DEG C for 2 h, taking the obtained supernatant, adding 3% (g/ml) of NaCl, allowing the obtained solution to stand at 90 DEG C for 20 min, and filtering the solution; and carrying out enzymatic hydrolysis by using pancreatin. The extraction method greatly improves the content of an effective component hexosamine in the above product. The chondroitin sulfate (CS) is connected with serine residues of protein molecules through oligomeric glucosides, so the CS released in the diluted alkali-enzymatic hydrolysistechnology may be still connected with a plurality of amino acid residues or polypeptides due to the certain specificity of enzyme hydrolysis peptide chains. The extraction method reduces the use amount of protease through setting the heating salt precipitation step in order to reduce the production cost, so the problem of slight high nitrogen content of the above product is solved to a certain degree.

Description

technical field [0001] The invention relates to a method for extracting chondroitin sulfate, in particular to a method for extracting chondroitin sulfate from sturgeon fish bones, the chondroitin sulfate extracted by the method and its application. Background technique [0002] Chondroitin sulfate (CS for short) is an acidic mucopolysaccharide belonging to glycosaminoglycans. It is generally white or yellowish powder with a slightly alkaline taste. Depending on the type of uronic acid in the molecular structure and the position of the sulfate on the hexosamine, chondroitin sulfate has multiple isomers such as CS—A, B, C, D, and E. At present, CS is mainly used clinically for the treatment of some nerve headaches, neuralgia, migraine, atherosclerosis, rheumatism, rheumatoid, hearing impairment caused by streptomycin, as well as adjuvant treatment of hepatitis and tumors. In addition, CS is also an important raw material for health care products, cosmetics, and pharmaceutical...

AI Technical Summary

Problems solved by technology

A comprehensive comparison of the literature at home and abroad shows that although the extraction speed is faster using concentrated alkali and medium alkali extraction processes, the quality of chondroitin sulfate is poor, the color is yellow or light red, and it is easy to deteriorate, and the drug consumption during the extraction process is relatively low. large, heavily polluted

Images