Preparation method and application of anti-oxidation active component of bamboo willows

An antioxidant activity and preparation technology, which is applied in the directions of medical preparations containing active ingredients, antidote, drug combination, etc. good effect

Active Publication Date: 2017-02-22
BIOLOGY INST OF SHANDONG ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this invention, only salicin in bamboo willow is extracted, but other active ingredients in bamboo willow are not extracted.
And in the prior art, there is no relevant repo

Method used

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  • Preparation method and application of anti-oxidation active component of bamboo willows
  • Preparation method and application of anti-oxidation active component of bamboo willows
  • Preparation method and application of anti-oxidation active component of bamboo willows

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The antioxidant active components of bamboo willow were extracted with n-butanol, and the steps were as follows:

[0028] A) Extraction: Accurately weigh 100 g of bamboo willow bark powder sieved through a 24-mesh sieve after crushing, add 10 times the amount of 70% ethanol solution as an extraction solvent, and perform ultrasonic extraction 3 times, each time for 60 min, at an ultrasonic temperature of 30° C., filter, Combine the extracts, concentrate under reduced pressure at 45°C until no ethanol remains, add 80 mL of distilled water to dissolve to make a 100 mg / mL sample solution, extract 5 times with the same volume of ethyl acetate, discard the ethyl acetate layer, add the same The volume of water-saturated n-butanol was extracted three times, and the extracts were combined, concentrated under reduced pressure at 40°C to a certain volume, and vacuum-dried at 800Pa and 45°C for 150min;

[0029] B) Purification of Sephadex LH-20: The diameter-to-height ratio of the ...

Embodiment 2

[0032] Use n-butanol to extract the antioxidant active components of bamboo willow, the steps are as follows: A) extraction: accurately weigh 100 g of bamboo willow bark powder that has been crushed and passed through a 40-mesh sieve, add 5 times the amount of 70% ethanol solution as the extraction solvent, and ultrasonically Extract 3 times, 45min each time, ultrasonic temperature is 30°C, filter, combine the extracts, concentrate under reduced pressure at 45°C until no ethanol remains, add 80mL distilled water to dissolve to make a 100mg / mL sample solution, and use the same volume of ethyl acetate Extract 4 times, discard the ethyl acetate layer, add the same volume of water-saturated n-butanol as the water layer and extract 3 times, combine the extracts, concentrate under reduced pressure at 40°C to a certain volume, and vacuum-dry at 800Pa, 45°C for 150min ;

[0033] B) Purification of Sephadex LH-20: The diameter-to-height ratio of the gel column is 1:18, and the powder o...

Embodiment 3

[0036] Use n-butanol to extract the antioxidant active components of bamboo willow, the steps are as follows: A) extraction: accurately weigh 100 g of bamboo willow bark powder that has been crushed and passed through a 10-mesh sieve, add 20 times the amount of 70% ethanol solution as the extraction solvent, and ultrasonically Extract 4 times, 90min each time, ultrasonic temperature is 30°C, filter, combine the extracts, concentrate under reduced pressure at 45°C until no ethanol remains, add 80mL distilled water to dissolve to make a 100mg / mL sample solution, and use the same volume of ethyl acetate Extract 5 times, discard the ethyl acetate layer, add the same volume of water-saturated n-butanol as the water layer and extract 3 times, combine the extracts, concentrate under reduced pressure at 40°C to a certain volume, and vacuum-dry at 800Pa, 45°C for 150min ;

[0037] B) Purification of Sephadex LH-20: The diameter-to-height ratio of the gel column is 1:18. Dissolve the po...

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Abstract

The invention provides a preparation method and application of an anti-oxidation active component of bamboo willows. The preparation method takes bamboo willow peels as raw materials and comprises the following steps: carrying out ultrasonic extraction for 1-5 times by utilizing 5 times to 20 times of ethanol with the concentration of 70% to obtain an extracting solution, wherein each time of extraction is carried out for 30min-120min; decompressing and concentrating the extracting solution until no ethanol is remained; adding distilled water and dissolving; extracting by utilizing ethyl acetate and water saturated n-butanol; decompressing, concentrating and drying an n-butanol extracting solution to obtain crude extract powder; and purifying the crude extract powder to obtain the anti-oxidation active component of the bamboo willows. The active component has a very strong cleaning effect on ABTS (2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate))+.free radicals and DPPH (1,1-diphenyl-2-picrylhydrazyl)+.free radicals; the activity of superoxide dismutase (SOD) in mouse tissues can be improved; and the damages to free radicals are inhibited and lipid peroxidation is prevented. A human body test shows that the active component can be directly used as a prescribed preparation for treating diseases, such as hyperlipidemia, caused by the fact that the free radicals in the human body are excessive. According to the preparation method and application of the anti-oxidation active component of bamboo willows, the anti-oxidation active component is successfully extracted from the bamboo willows and a foundation is laid for application of the bamboo willows to the field of medical products.

Description

technical field [0001] The invention relates to a preparation method and application of a bamboo willow antioxidant active component. The extract is separated from the n-butanol part of bamboo willow bark, has antioxidant activity, and belongs to the field of extraction, separation and purification of active ingredients of natural products. Background technique [0002] Bamboo willow (Salix ssp.), also known as American bamboo willow, is a plant of the genus Salix in the Salix family (Saliaceae). Traditional Salix plants have many excellent qualities, and at the same time show the advantages of fast growth, good material quality, and strong resistance (Zhang Jian et al., 2009), and are commonly used fast-growing timber forest species and biomass energy tree species (Xu Keshun, 2011). [0003] In recent years, the planting area of ​​bamboo and willow has expanded rapidly in my country. According to statistics, its forest area has exceeded 1300hm 2 (Hu Guotao et al., 2016), b...

Claims

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Application Information

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IPC IPC(8): A61K36/76A61P39/06A61P3/06
Inventor 张姗姗王荣春何秋霞韩利文刘可春王莹韩拥政王希敏李晓彬张轩铭侯海荣郭敬兰王雪王丽娟
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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