Nitrate reducing bacterium and application thereof

A technology of nitrate and microbial strains, applied to nitrate-reducing bacteria and its application fields, can solve problems such as secondary pollution, and achieve the effects of reducing crop nitrogen content, soil nitrate nitrogen content, and soil nitrogen loss

A technology of nitrate and microbial strains, applied to nitrate-reducing bacteria and its application fields, can solve problems such as secondary pollution, and achieve the effects of reducing crop nitrogen content, soil nitrate nitrogen content, and soil nitrogen loss

CN106434459AActive Publication Date: 2017-02-22GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Nitrate reducing bacterium and application thereof
  • Nitrate reducing bacterium and application thereof
  • Nitrate reducing bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Cultivation, isolation, screening and identification of Bacillus Nitratireductor lacus GSS14

[0011] 1.1 Culture and isolation of strains

[0012] The bacillus N.lacus GSS14 of the present invention is obtained by separating and purifying the bottom mud of Yuncheng Salt Lake in Shanxi.

[0013] Concrete separation and purification method comprises the following steps:

[0014] (1) Take 5.0g of Shanxi Yuncheng Salt Lake bottom mud and inoculate it into the enrichment and separation medium with a pH value of 7.2, culture at 30°C with shaking at 200rpm / min; after culturing for 3 days, transfer the culture medium to Transfer to another fresh enrichment and separation medium, and culture with shaking at 30°C and 200rpm / min.

[0015] (2) After four generations of enrichment culture, the culture solution of the fourth generation was diluted to 10 by the dilution plate method. -5 -10 -6 , spread 0.1 mL of the diluted culture solution evenly on the agar solid med...

Embodiment 2

[0031] Embodiment 2: Nitrate reducing effect of nitrate reducing bacteria N.lacus GSS14

[0032] (1) Pick a ring of bacterial lawn from the inclined surface of N.lacus GSS14 and put it into an Erlenmeyer flask equipped with LB liquid medium, and cultivate it on a shaking table at 37°C and 200rpm / min for 36h, and the fermented liquid is the seed liquid.

[0033] Glucose, sodium acetate, sodium lactate and other carbon sources were added to the basic culture of Giltay, and the C / N ratio in the culture medium was adjusted to 3:1. Divide the above culture solution into 500mL Erlenmeyer flasks, 100mL per bottle, inoculate 1mL of N.lacus GSS14 inoculum solution, culture in sequence batch at 37°C, 200rpm / min shaker, detect nitrate nitrogen and ammonia in the culture solution nitrogen content. Ammonia production rate = (NH 4 -N content / NO 3 -N initial concentration) / NO 3 -N initial concentration ×100.

[0034] The formula of the 2216E liquid medium is: yeast powder 1.0g, peptone 5...

Embodiment 3

[0037] Embodiment 3: the preparation of microbial bacterial agent

[0038] (1) Shake flask culture: Pick a ring of bacterial lawn from the N.lacus GSS14 slant and transfer it into a conical flask filled with 100mL 2216E liquid medium, culture it on a shaker at 37°C and 200rpm / min for 24h. The formula of the 2216E liquid medium described is: yeast powder 1.0g, peptone 5.0g, ferric citrate 0.1g, NaCl 19.45g, MgCl 2 5.98g, Na 2 SO 4 3.24g, CaCl 2 1.8g, KCl 0.55g, Na 2 CO 3 0.16g, BrCl 2 0.08g, SeCl 2 0.034g, H 3 BO 3 0.022g, Na 2 SiO 3 0.004g, NaF 0.0024g, NH 4 NO 3 0.0016g, Na 2 HPO 4 0.008g, water 1000mL, pH 7.2.

[0039] (2) Fermentation in seed tank: put the cultured N.lacus GSS14 fermentation broth into a 10L fermenter, and ferment at 37°C and 200 rpm / min for 36 hours; the formula of the fermentation medium used is the above-mentioned liquid medium.

[0040] (3) Fermentation in a fermenter: put the cultured N.lacus GSS14 fermentation broth into a fer...

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Abstract

The invention discloses a nitrate reducing bacterium and application thereof. The bacterial strain is named as Nitratireductor lacus in a classified mode, and preserved in the Guangdong microbial strain preservation center located on the fifth floor of the building No.59 in a courtyard on No.100, the middle of Guangzhou martyrs, and the preservation number is GDMCC No:60065. The bacterial strain Nitratireductor lacus can reduce nitrate in soil into ammoniacal nitrogen, reduce soil nitrogen losses and decrease the crop nitrogen content, and has the multiple effects of saving fertilizer, improving the crop quality and the like. The bacterial strain Nitratireductor lacus can be applied to biological nitrogen fixation, agricultural production and microbial preparation, and the bacterial strain and the microbial preparation thereof can be used for soil nitrogen fixation.

Description

technical field [0001] The invention relates to a new strain of bacteria and its application in the field of environmental engineering, in particular to a strain of nitrate-reducing bacteria and its application. Background technique [0002] Nitrogen is an important nutrient element, a component of amino acids and proteins in plants, and a component of chlorophyll, which plays a decisive role in photosynthesis in plants, and plays a very important role in crop growth. Therefore, more and more people understand and recognize that increasing nitrogen fertilizer as the main yield increasing factor in agricultural production. The application of nitrogen fertilizers can significantly increase yield, but excess nitrogen in farmland is lost from the soil through ammonia volatilization, nitrification / denitrification, etc., resulting in increased eutrophication of lakes, rivers and other water bodies. Among them, nitrate nitrogen has become the main form of leaching loss in fertiliz...

Claims

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Application Information

Patent Timeline
22 Feb 2017
Publication
CN106434459A
IPC
C12N1/20; C09K17/14; C12R1/01; C09K101/00
CPC
C09K17/14; C09K2101/00; C12N1/20; C12N1/205; C12R2001/01
Inventors
余震; 王跃强