Gastrodia tuber protein peptide effervescent tablets and preparation method thereof
A protein peptide, effervescent tablet technology, applied in the function of food ingredients, food science, application and other directions, can solve the problem of rare gastrodia elata products, and achieve the effect of good disintegration effect, easy absorption and good taste.
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[0029] The preparation method of the gastrodia protein peptide effervescent tablet comprises the following steps:
[0030] 1) Preparation of gastrodia protein peptide chitosan inclusion compound: Gastrodia protein peptide was prepared by enzymatic hydrolysis followed by ultrafiltration enzyme-membrane coupling technology, and then clathrated with chitosan. The specific steps are as follows:
[0031] 1.1) Enzymatic hydrolysis of Gastrodia elata protein: wash fresh Gastrodia elata to remove impurities, add water to beat, add water until the total amount of water added is 5 times the weight of fresh Gastrodia elata, and then add 20000-23000 U / g of protease Protomax in terms of protein content to adjust The temperature is 50°C-55°C, the pH is 7.0-7.5, and the enzyme is hydrolyzed for 3-4 hours while stirring. After heating at 140°C for 0.8 minutes to inactivate the enzyme, centrifuge at a speed of 7200r / min for 10-15 minutes, and take supernatant to obtain Gastrodia elata protein ...
Embodiment 1
[0041] 1. Preparation of gastrodia protein peptide
[0042] 1.1) Wash 100kg of fresh Gastrodia elata to remove impurities, add an appropriate amount of water to make a slurry, add water until the total amount of water added is 5 times the weight of fresh Gastrodia elata, and then add protease Protomax with a protein content of 20000-23000 U / g to adjust the temperature 50°C, pH 7.0, enzymatic hydrolysis for 4 h while stirring, heating at 140°C for 0.8 min to inactivate the enzyme, centrifuging at 7200 r / min for 10 min, taking the supernatant to obtain the Gastrodia elata protein enzymatic hydrolyzate;
[0043] 1.2) Purification of gastrodia protein peptide: filter the gastrodia protein hydrolyzate prepared in step 1.1) with an ultrafiltration membrane with a molecular weight of 2000Da, collect the part with an ultrafiltration molecular weight less than 2000Da, and then use a nanofiltration membrane with a molecular weight of 150Da for nanofiltration treatment Desalination and n...
Embodiment 2
[0054] 1. Preparation of gastrodia protein peptide
[0055] 1.1) Wash 100kg of fresh Gastrodia elata to remove impurities, add appropriate amount of water for beating, add water until the total amount of water added is 5 times the weight of fresh Gastrodia elata, then add protease Protomax with a protein content of 23000 U / g, and adjust the temperature to 55 ℃, pH 7.5, enzymatic hydrolysis for 3 h while stirring, heating at 140 ℃ for 0.8 min to inactivate the enzyme, centrifuging at 7200 r / min for 15 min, taking the supernatant to obtain Gastrodia elata protein enzymatic hydrolyzate;
[0056] 1.2) Purification of gastrodia protein peptide: filter the gastrodia protein hydrolyzate prepared in step 1.1) with an ultrafiltration membrane with a molecular weight of 3000Da, collect the part with an ultrafiltration molecular weight less than 3000Da, and then use a nanofiltration membrane with a molecular weight of 200Da for nanofiltration treatment Desalination and nanofiltration con...
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