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Graphene oxide composite nanomaterials functionalized with phenylboronic acid and its preparation and application

A technology of graphene composite and nanomaterials, applied in alkali metal compounds, chemical instruments and methods, alkali metal oxides/hydroxides, etc., can solve the problem of low affinity of phenylboronic acid monomer, weak binding ability, and glycoprotein selectivity No high problems, to achieve the effect of simple preparation, mild conditions, and environmental friendliness

Active Publication Date: 2018-11-27
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional phenylboronic acid monomer (PBA) has low affinity and weak binding ability to cis-o-diol, resulting in low selectivity to glycoproteins.

Method used

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  • Graphene oxide composite nanomaterials functionalized with phenylboronic acid and its preparation and application
  • Graphene oxide composite nanomaterials functionalized with phenylboronic acid and its preparation and application
  • Graphene oxide composite nanomaterials functionalized with phenylboronic acid and its preparation and application

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Experimental program
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Effect test

Embodiment 1

[0028] 1. Fe 3 o 4 Preparation of / PDA / GO / BPEI / CBX: (1) Weigh 1.08g of FeCl 3 ·6H 2 O and 0.2g of trisodium citrate, add 20ml of ethylene glycol, and ultrasonically disperse evenly. Add 1.2 g of anhydrous sodium acetate, and stir magnetically for at least 30 min. Finally, it was transferred to the reactor and reacted at 200°C for 10h. After the reaction was completed, the product was washed three times with deionized water and absolute ethanol, and vacuum-dried to obtain Fe 3 o 4 . (2) Take 100mg of Fe 3 o 4 , add 50ml of 10mM Tris-HCl buffered saline solution with pH=8.5, and disperse evenly by ultrasonic. Quickly add 100 mg of dopamine hydrochloride (DA), and bathe in water at 25°C for 2 hours to obtain Fe 3 o 4 / PDA. After the reaction was completed, the product was washed three times each with deionized water and absolute ethanol, and dried in vacuum. (3) Prepare 1mg / ml GO aqueous solution, and evenly dispersed Fe 3 o 4 / PDA aqueous solution, the mass ratio ...

Embodiment 2

[0031] 1. Fe 3 o 4 Preparation of / PDOPA / GO / PAMAM / CBX: (1) Weigh 1.08g of FeCl 3 ·6H 2O and 0.2g of trisodium citrate, add 20ml of ethylene glycol, and ultrasonically disperse evenly. Add 1.2 g of anhydrous sodium acetate, and stir magnetically for at least 30 min. Finally, it was transferred to the reactor and reacted at 200°C for 10h. After the reaction was completed, the product was washed three times with deionized water and absolute ethanol, and vacuum-dried to obtain Fe 3 o 4 . (2) Take 100mg of Fe 3 o 4 , add 50ml of 10mM Tris-HCl buffered saline solution with pH=8.5, and disperse evenly by ultrasonic. Quickly add 100 mg of 3,4-dihydroxyphenylalanine (DOPA), and bathe in water at 25°C for 2 hours to obtain Fe 3 o 4 / PDOPA. After the reaction was completed, the product was washed three times each with deionized water and absolute ethanol, and dried in vacuum. (3) Prepare 1mg / ml GO aqueous solution, and evenly dispersed Fe 3 o 4 / PDOPA aqueous solution, the...

Embodiment 3

[0035] 1. Fe 3 o 4 Preparation of / PDA / GO / BPEI / CPBA: (1) Weigh 1.08g of FeCl 3 ·6H 2 O and 0.2g of trisodium citrate, add 20ml of ethylene glycol, and ultrasonically disperse evenly. Add 1.2 g of anhydrous sodium acetate, and stir magnetically for at least 30 min. Finally, it was transferred to the reactor and reacted at 200°C for 10h. After the reaction was completed, the product was washed three times with deionized water and absolute ethanol, and vacuum-dried to obtain Fe 3 o 4 . (2) Take 100mg of Fe 3 o 4 , add 50ml of 10mM Tris-HCl buffered saline solution with pH=8.5, and disperse evenly by ultrasonic. Quickly add 100 mg of dopamine hydrochloride and bathe in water at 25°C for 2 hours to obtain Fe 3 o 4 / PDA. After the reaction was completed, the product was washed three times each with deionized water and absolute ethanol, and dried in vacuum. (3) Prepare 1mg / ml GO aqueous solution, and evenly dispersed Fe 3 o 4 / PDA aqueous solution, the mass ratio of th...

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Abstract

The invention relates to a novel graphene oxide composite nano material functionalized with phenylboronic acid and its preparation and application. The material described is to immobilize polydopamine-wrapped magnetic nanoparticles onto polyethyleneimine-modified graphene oxide, further introduce carboxyl-containing phenylboronic acid monomers through polyethyleneimine amino groups, and finally be used for glycoprotein-rich set. The specific process is as follows: firstly, ferroferric oxide magnetic nanoparticles are prepared by a solvothermal method, and dopamine can self-polymerize on the surface under alkaline conditions. Magnetic graphene oxide composite nanomaterials were prepared by using hydrogen bonds and π-π interactions between polydopamine and graphene oxide. Then, positively charged polyethyleneimine was immobilized onto the negatively charged magnetic graphene oxide surface through electrostatic self-assembly. Finally, phenylboronic acid monomers were introduced through amidation reaction to obtain phenylboronic acid-functionalized magnetic graphene oxide composite nanomaterials, which were successfully used for the enrichment of glycoproteins in bioanalysis.

Description

technical field [0001] The invention relates to a graphene oxide composite nanometer material functionalized with phenylboronic acid and its preparation and application to the specific enrichment of glycoproteins. Background technique [0002] The boron affinity strategy has attracted more and more attention in the separation and enrichment of glycosylated proteins because of its simple operation, unbiasedness, and non-destructive glycoform structure. However, the traditional phenylboronic acid monomer (PBA) has low affinity and weak binding ability to cis-o-diols, resulting in low selectivity to glycoproteins. According to literature reports, a new type of phenylboronic acid (2-hydroxymethylphenylboronic acid cyclic monoester and its derivatives) has a low pKa value (pKa=7.3), and its binding ability with cis-o-diol is higher than that of traditional phenylboronic acid Higher, so it is a very potential new monomer, which has been successfully applied to the enrichment of g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30
Inventor 张丽华吴琼江波翁叶靖张玉奎
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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