Preparation method of oxytocin [4-Glu]

An oxytocin and chromatography technology, applied in the field of biopharmaceuticals, can solve the problems of dilute sample concentration, low yield, complicated preparation process, etc., and achieve the effect of optimizing the production process

Active Publication Date: 2017-03-08
SPH NO 1 BIOCHEM & PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved by the present invention is to provide a kind of oxytocin [4-Glu] in order to overcome the defects of c

Method used

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  • Preparation method of oxytocin [4-Glu]
  • Preparation method of oxytocin [4-Glu]
  • Preparation method of oxytocin [4-Glu]

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 (HPLC method detects the purity and quantification of oxytocin [4-Glu] precursor crude product and purified intermediate solution)

[0033] Instrument: Agilent 1200 HPLC

[0034] Separation column: Waters XBridge-C18, 4.6×150 mm, 5 μm

[0035] Mobile phase: A is 50% by volume acetonitrile aqueous solution, B is 0.02M KH 2 PO 4 pH 3.0 aqueous solution, flow rate 1.0 mL / min, detection wavelength 220nm, room temperature detection, elution gradient as shown in the table below, percentages are volume percentages.

[0036] Elution step Elution time eluent 1 0~15min 35%A+65%B 2 15~25min 35%A+65%B→100%A 3 25~28min 100％A 4 28.0~28.1min 100%A→35%A+65%B 5 28.1~32min 35%A+65%B

Embodiment 2

[0037] Embodiment 2 (50mm inner diameter L&L4002 preparative column packing)

[0038] Using Load&Lock dynamic axial compression and static locking technology, the filler is silica gel C18 (Kromasil C18), the pore size is 10nm, the particle size is 10μm, the column packing density is 0.66 g / mL, and the column bed pressure is 133bar, and the Varian chromatography packing system is used, 325g Dry powder filler, 650mL of isopropanol is stirred and homogenized, poured into L&L4002 preparative column with an inner diameter of 50mm, the compression ratio is 1.5:1, and the carrier gas is N 2 , adjust the carrier gas pressure so that the pressure of the oil pressure gauge is 200bar, and the dynamic axial compression to the height of the column bed is 25.5cm, which is used as the preparative column for the reverse-phase cyclization, reverse-phase purification and reverse-phase desalination schemes.

Embodiment 3

[0039] Example 3 Reverse-phase cyclization, reverse-phase purification and reverse-phase desalting of oxytocin [4-Glu] precursor raw material

[0040] Instrument: Varian SD-1 high pressure liquid phase preparation system

[0041] Chromatographic column: Example 2 self-packed preparative column Load&Lock4002 50×255 mm, C18 10μm10nm

[0042]The crude product of oxytocin [4-Glu] precursor is the crude product of oxytocin [4-Glu] precursor obtained by cracking and drying by solid-phase synthesis method, and the structural formula is Cys-Tyr-Ile-Glu-Asn-Cys-Pro -Leu-Gly-NH 2 of trifluoroacetate.

[0043] The crude oxytocin [4-Glu] precursor solution is a 5 g / L solution formed by dissolving the above crude oxytocin [4-Glu] precursor in 5% acetonitrile aqueous solution.

[0044] Mobile phase A1 is purified water, A2 is 0.02 volume percent H 2 o 2 pH is the NaOH aqueous solution of 7.5, and mobile phase B is acetonitrile, and mobile phase C is described oxytocin [4-Glu] precurso...

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Abstract

The invention discloses a preparation method of oxytocin [4-Glu]. The preparation method comprises steps as follows: a precursor crude product solution of oxytocin [4-Glu] is sequentially subjected to reversed-phase cyclization, reversed-phase purification and reversed-phase desalination with a high-performance liquid reversed-phase chromatography method; filler of the high-performance liquid reversed-phase chromatography method is silicone C18; a precursor crude product of oxytocin [4-Glu] contains two free sulfydryls. The problems about cyclization, purification and desalination are solved once with an innovative reversed-phase adsorption method for cyclization, purification and desalination, the production process is optimized, and the method is suitable for industrial continuous production.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals. More specifically, the present invention relates to a preparation method of oxytocin [4-Glu]. Background technique [0002] Oxytocin, also known as oxytocin, English name is Oxytocin, structural formula: [0003] [0004] The molecular formula is: C 43 h 66 N 12 o 12 S 2 , the molecular weight is 1007.2 [0005] Oxytocin is used to induce labor, oxytocin, postpartum and post-abortion uterine bleeding caused by uterine atony or poor abdominal contraction; understand placental reserve function (oxytocin provocation test); nasal drops can promote milk discharge. Oxytocin can indirectly stimulate the contraction of uterine smooth muscle, simulating the uterine contraction of normal childbirth, leading to cervical dilatation, and the uterine response to oxytocin gradually increases during pregnancy and reaches its peak at full term. Oxytocin can also stimulate the contraction of the smooth...

Claims

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Application Information

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IPC IPC(8): C07K7/16C07K1/20C07K1/04
CPCC07K7/16
Inventor 江锡铭丁金国黄臻辉
Owner SPH NO 1 BIOCHEM & PHARMA CO LTD
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