Identification method for urine metabolism marker based on alzheimer disease

A metabolic marker, Alzheimer's disease technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problem of inaccurate identification of urinary metabolic markers.

Active Publication Date: 2017-03-15
王喜军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to solve the shortcomings that the existing urine metabolic markers of Alzheimer's disease cannot be accurately identified, and propose a method for identifying urine metabolic markers based on Alzheimer's disease

Method used

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  • Identification method for urine metabolism marker based on alzheimer disease
  • Identification method for urine metabolism marker based on alzheimer disease
  • Identification method for urine metabolism marker based on alzheimer disease

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Experimental program
Comparison scheme
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specific Embodiment approach 1

[0042] Specific embodiment one: the specific process of a kind of urine metabolic marker identification method based on Alzheimer's disease of the present embodiment is:

[0043] Step 1, establishment and evaluation of senile dementia rat model;

[0044] Step 11, using intraperitoneal injection of D-galactose combined with gavage of aluminum trichloride to induce rats to establish an AD rat model;

[0045] Step 12, using behavioral, histopathological and clinical biochemical indicators to evaluate the establishment process of the AD rat model;

[0046] Step 2. Using biomarkers to evaluate the intervention effect of Kaixin San on AD rat model establishment at different stages;

[0047] Step 21, prepare Kaixinsan freeze-dried powder and Kaixinsan intragastric solution, and obtain rats containing Kaixinsan in their bodies;

[0048] Step 22, grouping and designing blank group rats and D rat models;

[0049] Take 100 9-week-old male Wistar rats (body weight 260±20g) and adapt to...

specific Embodiment approach 2

[0056] Specific embodiment two: the difference between this embodiment and specific embodiment one is that in the step one by one, the rats are induced by intraperitoneal injection of D-galactose combined with intragastric gavage of aluminum trichloride to establish an AD rat model; the specific process is as follows: :

[0057] experimental animals

[0058] Wistar rats, male, 9 weeks old, weighing 260±20g, were provided by the Animal Experiment Center of Heilongjiang University of Traditional Chinese Medicine. Raised at room temperature, the animals had free access to food and water.

[0059] 1.1 Experimental method

[0060] 1.1.1 Experimental grouping and design: 70 Wistar rats were acclimated to the environment for a week. First, the animals were screened by the Morris water maze to remove those with congenital dementia (average latency >50s) and poor swimming posture. The qualified rats were randomly divided into There were 10 rats in the blank control group and 10 rats...

specific Embodiment approach 3

[0081] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: in the step 21, prepare Kaixinsan freeze-dried powder and Kaixinsan intragastric solution, and obtain rats containing Kaixinsan in the body; the specific process is :

[0082] Step 211, preparation of Kaixinsan freeze-dried powder;

[0083] According to the proportion of the original recipe, that is, the coarse powder of medicinal materials is in the ratio of parts by mass: ginseng: Poria cocos: polygala: calamus = 3:3:2:2, weigh 500g coarse powder of medicinal materials, mix well, and use 6 times the mass concentration Heat and reflux extraction with 70% ethanol twice, 2 hours each time, combine the filtrates, concentrate, and dry by freeze-drying method to obtain loose powder, seal and place in a desiccator for later use and calculate the powder yield, the powder yield is 23.4%; Calculated as 4 capsules per 1g, the dose of crude drug per person per day is 21 capsu...

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Abstract

The invention relates to an identification method for a urine metabolism marker based on alzheimer disease. The identification method aims at solving the problem that a urine metabolism marker of existing alzheimer disease has not been clarified. The identification method for the urine metabolism marker based on alzheimer disease includes the specific steps that 1, alzheimer rat model is built and evaluated; 2, the intervention effect of Kaixin powder on building different-stage AD fat models is evaluated through the biomarker. The identification method is used for the field of metabolism marker identification.

Description

technical field [0001] The invention relates to a method for identifying urine metabolic markers based on Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is a chronic neurodegenerative disease. AD not only seriously affects human health and quality of life, but also brings a heavy burden to patients' families and society. AD is a complex disease, and its occurrence and development is a continuous pathophysiological process. Due to the irreversibility of its onset, the window of basic and clinical research on AD should be moved forward. Early intervention is especially important. Currently, the diagnostic criteria for AD mainly include the Mini-Mental State Evaluation Examination (MMSE) test of the scale, and neuronal imaging of amyloid-β. Kaixin Powder is the basic prescription of traditional Chinese medicine for improving intelligence, calming the nerves and calming convulsions, and treating amnesia. Syndrome such as timidity is a classic pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张爱华初航孙晖王喜军
Owner 王喜军
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