Mercury ion probe based on doped ion luminescent mechanism and its synthesis method and application
A mercury ion and fluorescent probe technology, applied in the nanometer field, can solve the problems affecting the accuracy of the probe, and achieve the effects of fast reaction speed, good selectivity, and wide linear range
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Embodiment 1
[0030] Embodiment 1: aqueous phase synthesis Mn:ZnSe quantum dot
[0031] 31.6mg (0.4mol) Se powder and 37.8mg (1.0mol) NaBH 4 The solids were mixed evenly, injected with 2 mL of ultrapure water, and stirred at 25°C for 1 h to obtain a colorless and transparent NaHSe solution.
[0032] 0.0025mmol of Mn(OAc) 2 Dissolve in 10mL water together with 3mmol mercaptopropionic acid, adjust the pH of the solution to 7, then pass nitrogen gas to the mixed solution for 30min to remove oxygen, then add 0.063mmol newly prepared NaHSe solution to obtain the MnSe nanoparticle dispersion; MnSe nanoparticles Heat the dispersion to 100°C, then add 0.125mmol Zn(OAc) 2 React for 30 minutes to obtain a Mn:ZnSe quantum dot dispersion; centrifuge the obtained Mn:ZnSe quantum dot dispersion, dialyze, and vacuum dry for 48 hours to obtain solid Mn:ZnSe quantum dots. After measurement, the particle size of the Mn:ZnSe quantum dots prepared in Example 1 is 2nm, and the fluorescence intensity of the M...
Embodiment 2
[0033] Embodiment 2: aqueous phase synthesis Mn:ZnSe quantum dot
[0034] 31.6mg (0.4mol) Se powder and 37.8mg (1.0mol) NaBH 4 The solids were mixed evenly, injected with 2 mL of ultrapure water, and stirred at 25°C for 1 h to obtain a colorless and transparent NaHSe solution.
[0035] 0.0025mmol of Mn(OAc) 2 Dissolve 3mmol of mercaptopropionic acid in 10mL of water, adjust the pH of the solution to 8, and then pass nitrogen gas to the mixed solution for 30min to remove oxygen; then add 0.063mmol of newly prepared NaHSe solution to obtain a dispersion of MnSe nanoparticles; MnSe nanoparticles Heat the dispersion to 100°C, then add 0.125mmol Zn(OAc) 2 React for 30 minutes to obtain a Mn:ZnSe quantum dot dispersion; centrifuge the obtained Mn:ZnSe quantum dot dispersion, dialyze, and vacuum dry for 48 hours to obtain solid Mn:ZnSe quantum dots.
Embodiment 3
[0036] Embodiment 3: aqueous phase synthesis Mn:ZnSe quantum dot
[0037] 31.6mg (0.4mol) Se powder and 37.8mg (1.0mol) NaBH 4 The solids were mixed evenly, injected with 2 mL of ultrapure water, and stirred at 25°C for 1 h to obtain a colorless and transparent NaHSe solution.
[0038] 0.0025mmol of Mn(OAc) 2 and 3mmol of mercaptopropionic acid were dissolved in 10mL of water, and the pH of the solution was adjusted to 9. After the mixed solution was transferred to the reaction system, nitrogen gas was passed for 30min, and then 0.063mmol of newly prepared NaHSe solution was added to obtain a dispersion of MnSe nanoparticles; the MnSe nanoparticles Heat the dispersion to 100°C, then add 0.125mmol Zn(OAc) 2 React for 30 minutes to obtain a Mn:ZnSe quantum dot dispersion; centrifuge the obtained Mn:ZnSe quantum dot dispersion, dialyze, and vacuum dry for 48 hours to obtain solid Mn:ZnSe quantum dots.
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