Microbial preparation for decomposing straw cellulose, preparation method thereof, and method for decomposing straw cellulose
A microbial preparation and straw cellulose technology, applied in the field of microbial preparations, can solve problems such as unsatisfactory fermentation efficiency and general palatability of fermented products, and achieve the effect of improving palatability and nutrition, and good fermentation decomposition effect
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Embodiment 1
[0056] First, respectively configure the medium suitable for the growth of different strains;
[0057] Wherein, in the common liquid culture medium that Phanerochaete chrysosporium, Cellulomonas chrysogenum and Candida utilis are used to cultivate and content are: corn flour (5.0g / L), rice straw powder ( 3.0g / L), ammonium nitrate (1.0g / L), magnesium sulfate (0.1g / L), potassium dihydrogen phosphate (0.2g / L), dipotassium hydrogen phosphate (0.1g / L); trace elements: Fe 3+ (0.002mol / L), Mn 2+ (0.004mol / L), Zn 2+ (0.003mol / L).
[0058] The components and contents of various nutrients in the Lactobacillus acidophilus liquid medium are: corn flour 10g / L, diammonium citrate 2g / L, dipotassium hydrogen phosphate 2g / L, Tween80 2g / L, sodium acetate trihydrate 5g / L L.
[0059] Then, prepare the high-efficiency anaerobic straw starter of the present invention according to the method as follows:
[0060] 1) Preparation of primary liquid strains
[0061] i) Put the prepared common liqui...
experiment example 1
[0081] In summer, when the temperature is about 30°C, according to the present invention, the mass ratio of the microbial preparation for decomposing straw cellulose to the straw powder is 1:1000. After the microbial preparation and the straw powder are mixed, anaerobic fermentation is carried out for 7~ 10d; Then, the fermentation was stopped, and after inspection, it was found that the straw powder had turned into a paste, that is, the fermentation was completed.
[0082] The pasty fermented product and unfermented straw powder from the fermentation institute were used to feed yellow beef cattle of the same month age. It was found that the cattle had a higher preference for straw fermented product; The fed yellow beef cattle obviously gain more weight than the yellow beef cattle fed with unfermented stalks.
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