Novel immobilized enzyme preparation, and preparation method and application thereof

An immobilized enzyme, a new type of technology, applied in the direction of being immobilized on/in an organic carrier, can solve the problems of complex enzyme immobilization method, difficult to recycle, small load capacity, etc., and achieve high immobilization efficiency and high enzyme performance. Good stability and mild reaction effect

Inactive Publication Date: 2017-03-22
GUANGZHOU KAIYAO ASSET MANAGEMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the deficiencies and shortcomings of the enzyme immobilization methods in the prior art, which are too complicated, low in efficiency, high in cost, difficult to recycle, and small in loading capacity, the primary purpose of the present invention is to provide a method for preparing a novel immobilized enzyme preparation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Chitosan is dissolved in acetic acid solution to obtain chitosan acetic acid solution, wherein the massfraction of chitosan is 3%, and the massfraction of acetic acid is 5%; then add tetraoxide in chitosan acetic acid solution Three ferromagnetic fluid, 45 ℃ of ultrasonic reactions 50min, obtain product 1, wherein, the mass ratio of ferroferrofluid and chitosan acetic acid solution is 1:3; Product 1 is added liquid paraffin / Span-80 mixed liquor ( The volume ratio of liquid paraffin and Span-80 is 25:1), and the volume ratio of product 1 and liquid paraffin / Span-80 mixture is 1:4; Add the crosslinking agent glutaraldehyde under the condition (the consumption of glutaraldehyde is 8% of total reaction system volume), and adjust the pH of the reaction system to be 10, continue the reaction in a constant temperature water bath for 1.5h; then filter under reduced pressure, and use petroleum ether, Extracted with absolute ethanol 4 times, dried in vacuum at 50°C to obtain ...

Embodiment 2

[0049] (1) chitosan is dissolved in acetic acid solution to obtain chitosan acetic acid solution, wherein the massfraction of chitosan is 1%, and the massfraction of acetic acid is 1%; then add tetraoxide in chitosan acetic acid solution Three ferromagnetic fluid, 40 ℃ of ultrasonic reactions 60min, obtain product 1, wherein, the mass ratio of ferroferrofluid and chitosan acetic acid solution is 1:2; Product 1 is added liquid paraffin / Span-80 mixed liquor ( The volume ratio of liquid paraffin and Span-80 is 30:1), and the volume ratio of product 1 and liquid paraffin / Span-80 mixture is 1:5; Add cross-linking agent glutaraldehyde under the condition (the consumption of glutaraldehyde is 5% of total reaction system volume), and adjust reaction system pH to be 11, constant temperature water bath continues reaction 2h; Extracted with water and ethanol for 3 times, dried in vacuum at 30°C to obtain nano-magnetic chitosan;

[0050] (2) Dissolve γ-cyclodextrin and potassium benzoate...

Embodiment 3

[0055] (1) chitosan is dissolved in acetic acid solution to obtain chitosan acetic acid solution, wherein the massfraction of chitosan is 5%, and the massfraction of acetic acid is 10%; then add tetraoxide in chitosan acetic acid solution Three ferrofluid, 50 ℃ of ultrasonic reactions 40min, obtain product 1, wherein, the mass ratio of ferroferrofluid and chitosan acetic acid solution is 1:5; Product 1 is added liquid paraffin / Span-80 mixed liquor ( The volume ratio of liquid paraffin and Span-80 is 20:1), and the volume ratio of product 1 and liquid paraffin / Span-80 mixture is 1:3; Add cross-linking agent glutaraldehyde (the consumption of glutaraldehyde is 10% of total reaction system volume) under condition, and adjust reaction system pH to be 9, constant temperature water bath continues reaction 1h; Extracted with water and ethanol for 5 times, dried in vacuum at 60°C to obtain nano-magnetic chitosan;

[0056] (2) Dissolve γ-cyclodextrin and potassium benzoate in water at...

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PUM

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Abstract

The invention belongs to the field of biological catalysis, and particularly relates to a novel immobilized enzyme preparation, and a preparation method and application thereof. The preparation method of the novel immobilized enzyme preparation comprises the following steps: combining nano magnetic chitosan microspheres and a metal-organic framework material having so many properties such as multiple pores, large specific area, multiple metal sites and the like through graft copolymerization to obtain a nano magnetic chitosan-metal organic framework material having a core-shell structure with a porous network structure; and mixing the nano magnetic chitosan-metal organic framework material used as a carrier and an enzyme solution, and immobilizing at 0-25 DEG C for 1-12 hours to obtain the novel immobilized enzyme preparation. The method can immobilize enzymes such as lipase, proteinase, peroxidase and the like, and has the advantages of low cost, high immobilization efficiency, favorable enzyme stability, high enzyme loading amount and high enzyme recovery rate; no bifunctional reagent is used in the immobilization process; and the method is simple to operate, low in cost and mild in reaction.

Description

technical field [0001] The invention belongs to the field of biocatalysis, and in particular relates to a novel immobilized enzyme preparation and its preparation method and application. Background technique [0002] Enzymes are biocatalysts produced by living cells that catalyze specific biochemical reactions. Enzyme preparations are biological products with catalytic functions after purification and processing of enzymes. They are mainly used to catalyze various chemical reactions in the production process. They have high catalytic efficiency, high specificity, mild action conditions, reduced energy consumption, and reduced chemical Pollution and other characteristics, its application fields are all over food (bread baking industry, flour deep processing, fruit processing industry, etc.), textile, feed, detergent, papermaking, leather, medicine, energy development, environmental protection, etc. [0003] Immobilized enzyme is a new technology developed in the 1960s. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02C12N11/10
CPCC12N11/10C12N11/02
Inventor 曹蓓蓓
Owner GUANGZHOU KAIYAO ASSET MANAGEMENT CO LTD
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