Extraction method for tea polyphenol and theanine in tea old leaves, and processing method for tea dregs

An extraction method and technology for tea polyphenols are applied in the field of extraction of tea polyphenols and theanine in old tea leaves, which can solve the problems of not being effectively utilized, pending research and verification, and increasing tea yield, and achieve the realization of tea production. , Nutrient release, fast absorption effect

Active Publication Date: 2017-03-29
湖北宇隆生物工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the tea polyphenols and theanine used in the market are proposed from tea leaves, and the tea leaves after extraction are thrown away, the cost of raw materials is high, and the source of raw materials is limited; therefore, tea polyphenols and theanine The chemical synthesis method of acid not only requires high production input for the production of tea polyphenols and theanine, but also needs to be studied and verified whether the chemically synthesized tea polyphenols and theanine can be used in the food industry
[0003] After the tea tree is planted, the tea tree will be s

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0034] Example 1

[0035] A. Wash the old tea leaves picked from the twigs with water, spread the old tea leaves and dry the surface moisture;

[0036] B. Stir-fry the old tea leaves at a temperature of 65°C for 40 minutes. Dry the old tea leaves until the temperature of the old tea leaves reaches room temperature. After adding appropriate amount of cellulase, the old tea leaves are twisted into strips. , Knead until the water flow stops;

[0037] C. Fry the old tea leaves after kneading for a second time at a temperature of 45°C and a time of 50 minutes, and then spread to room temperature;

[0038] D. Take 5g tea leaves and 100ml distilled water, soak the tea leaves in distilled water at 65°C for 40 minutes, filter the tea dregs to obtain the extract, repeat the soaking twice, and combine the obtained extracts; determine by HPLC liquid chromatography , And calculated that the content of theanine is 0.7% of the dry tea quality, and the total amount of tea polyphenols is 9.7% of the ...

Example Embodiment

[0043] Example 2

[0044] A. Wash the old tea leaves picked from the twigs with water, spread the old tea leaves and dry the surface moisture;

[0045] B. Fry the old tea leaves once at a temperature of 70°C for 45 minutes, and air them until the temperature of the old tea leaves reaches room temperature. After adding appropriate amount of cellulase, the old tea leaves are rolled into strips. , Knead until the water flow stops;

[0046] C. Fry the old tea leaves after rolling for a second time at a temperature of 50°C and a time of 52 minutes, and then spread to room temperature;

[0047] D. Take 5g tea leaves and 100ml distilled water, soak the tea leaves in distilled water at 67°C for about 50 minutes, filter the tea dregs to obtain an extract, then repeat the soaking 3 times, and combine the obtained extracts; by HPLC liquid chromatography The content of theanine was determined and calculated to be 1.04% of the dry tea quality, and the total amount of tea polyphenols was determine...

Example Embodiment

[0052] Example 3

[0053] A. Wash the old tea leaves picked from the twigs with water, spread the old tea leaves and dry the surface moisture;

[0054] B. Fry the old tea leaves once at a temperature of 75°C for 50 minutes, and let them dry until the temperature of the old tea leaves reaches room temperature. After adding appropriate amount of cellulase, the old tea leaves are twisted into strips. , Knead until the water flow stops;

[0055] C. Fry the old tea leaves after kneading for a second time, the temperature is 55℃, and the time is 55 minutes, then spread to room temperature;

[0056] D. Take 5g tea leaves and 100ml distilled water, soak the tea leaves in distilled water at 70°C for about 60 minutes, filter the tea dregs to obtain an extract, repeat the soaking twice, and combine the obtained extracts; by HPLC liquid chromatography The content of theanine is determined and calculated to be 1.01% of the dry tea quality, and the total amount of tea polyphenols is 11.3% of the d...

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PUM

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Abstract

The invention discloses an extraction method for tea polyphenol and theanine in tea old leaves, and a method for preparing base fertilizer dedicated for tea trees by tea old leaf dregs. The theanine in tea old leaf leach liquor is adsorbed through cation exchange resin; then, citrate solution is adopted to carry out elution on the theanine; the tea polyphenol is adsorbed through adsorption resin, and then, the tea polyphenol is subjected to elution by ethanol water; the tea old leaf dregs obtained for preparing the leach liquor and yellow mealworm dungs are matched to carry out primary fermentation; under a function that substances including monopotassium phosphate, potassium chloride, ferrous sulfate, manganese sulfate and the like are added into Trichoderma reesei and Bacillus thermophiles, secondary fermentation is carried out; and then, diammonium phosphate, urea, ammonium molybdate and calcium superphosphate are added and evenly mixed for granulation to obtain the fertilizer dedicated for tea trees. The method provides a new application for the tea old leaves, and also provides a new raw material which can extract the tea polyphenol and the theanine, and waste materials, i.e., the extracted tea old leaf dregs, can be made good use.

Description

technical field [0001] The invention relates to a method for extracting bioactive substances from old tea leaves, in particular to a method for extracting tea polyphenols and theanine from old tea leaves. Background technique [0002] Tea polyphenols and theanine are important active ingredients in tea. With the continuous development of the chemical synthesis methods of folic acid, tea polyphenols and theanine, more and more functions and uses of tea polyphenols and theanine It was discovered and proven, therefore, that polyphenols and theanine are in great demand. Most of the tea polyphenols and theanine used in the market are proposed from tea leaves, and the tea residues after extraction are thrown away, the cost of raw materials is high, and the source of raw materials is limited; therefore, tea polyphenols and theanine The chemical synthesis method of acid not only requires high production input for the production of tea polyphenols and theanine, but also needs to be ...

Claims

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Application Information

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IPC IPC(8): C07C237/06C07C231/24C07G99/00C05G1/00C05F17/00A23L33/105A23L33/175
CPCC05B1/02C07C231/24C07C237/06A23V2002/00C05F17/50C07G99/00A23V2250/2132A23V2250/21C05B7/00C05C3/00C05C9/00C05D1/02C05D9/02C05F3/00C05F5/006C05F11/08Y02W30/40
Inventor 马喆杜琼
Owner 湖北宇隆生物工程有限责任公司
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