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Extraction method for tea polyphenol and theanine in tea old leaves, and processing method for tea dregs

An extraction method and technology for tea polyphenols are applied in the field of extraction of tea polyphenols and theanine in old tea leaves, which can solve the problems of not being effectively utilized, pending research and verification, and increasing tea yield, and achieve the realization of tea production. , Nutrient release, fast absorption effect

Active Publication Date: 2017-03-29
湖北宇隆生物工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the tea polyphenols and theanine used in the market are proposed from tea leaves, and the tea leaves after extraction are thrown away, the cost of raw materials is high, and the source of raw materials is limited; therefore, tea polyphenols and theanine The chemical synthesis method of acid not only requires high production input for the production of tea polyphenols and theanine, but also needs to be studied and verified whether the chemically synthesized tea polyphenols and theanine can be used in the food industry
[0003] After the tea tree is planted, the tea tree will be shaped and pruned and re-pruned, mainly for the side buds of the young tea tree to germinate, to increase the branch level and number of the tea tree, and to cultivate the backbone and skeleton of the tea tree; in addition, the tea is picked every year After that, there will be regular light pruning, mainly to prune the new shoots that have grown in the past one or two years, so as to increase the branches on the tea tree and increase the yield of tea; the old tea leaves on the pruned shoots have not yet been harvested. Efficient use, pruned shoots are generally used for fuel burning

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A. Wash the old tea leaves picked from the twigs with water, spread the old tea leaves and dry the surface moisture;

[0036] B. Fry the old tea leaves once at a temperature of 65°C for 40 minutes, let the old tea leaves reach normal temperature, add an appropriate amount of cellulase, and knead the old tea leaves into strips , knead until the water outflow stops;

[0037] C, the old tea leaf after kneading is carried out secondary frying, and frying temperature is 45 ℃, and frying time is 50 minutes, spread out to normal temperature then;

[0038] D, take 5g of tea leaves and 100ml of distilled water, soak the tea leaves in distilled water at 65°C for 40 minutes, filter the tea leaves to obtain the extract, repeat the soaking twice, and combine the obtained extracts; determine by HPLC liquid chromatography , and the calculated content of theanine is 0.7% of the dry tea quality, and the total amount of tea polyphenols obtained by using the ferrous tartrate method to me...

Embodiment 2

[0044] A. Wash the old tea leaves picked from the twigs with water, spread the old tea leaves and dry the surface moisture;

[0045] B. Fry the old tea leaves once at a temperature of 70°C for 45 minutes, let the old tea leaves reach normal temperature, add an appropriate amount of cellulase, and knead the old tea leaves into strips , knead until the water outflow stops;

[0046] C, the old tea leaf after kneading is carried out secondary frying, and frying temperature is 50 ℃, and frying time is 52 minutes, spreads out to normal temperature then;

[0047] D. Take 5g of tea leaves and 100ml of distilled water, soak the tea leaves in distilled water at 67°C for about 50 minutes, filter the tea leaves to obtain the extract, then repeat the soak for 3 times, and combine the obtained extracts; through HPLC liquid chromatography Measure and calculate the content of theanine to be 1.04% of the dry tea quality, and measure and calculate the total amount of tea polyphenols by using t...

Embodiment 3

[0053] A. Wash the old tea leaves picked from the twigs with water, spread the old tea leaves and dry the surface moisture;

[0054] B. Fry the old tea leaves once at a temperature of 75°C for 50 minutes, let the old tea leaves reach normal temperature, add an appropriate amount of cellulase, and knead the old tea leaves into strips , knead until the water outflow stops;

[0055] C, carry out secondary frying to the old tea leaves after kneading, the frying temperature is 55°C, the frying time is 55 minutes, then spread to normal temperature;

[0056] D. Take 5g of tea leaves and 100ml of distilled water, soak the tea leaves in distilled water at 70°C for about 60 minutes, filter the tea leaves to obtain the extract, repeat the soaking twice, and combine the obtained extracts; through HPLC liquid chromatography Measure and calculate the content of theanine to be 1.01% of the dry tea quality, and measure and calculate the total amount of tea polyphenols by using the ferrous ta...

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Abstract

The invention discloses an extraction method for tea polyphenol and theanine in tea old leaves, and a method for preparing base fertilizer dedicated for tea trees by tea old leaf dregs. The theanine in tea old leaf leach liquor is adsorbed through cation exchange resin; then, citrate solution is adopted to carry out elution on the theanine; the tea polyphenol is adsorbed through adsorption resin, and then, the tea polyphenol is subjected to elution by ethanol water; the tea old leaf dregs obtained for preparing the leach liquor and yellow mealworm dungs are matched to carry out primary fermentation; under a function that substances including monopotassium phosphate, potassium chloride, ferrous sulfate, manganese sulfate and the like are added into Trichoderma reesei and Bacillus thermophiles, secondary fermentation is carried out; and then, diammonium phosphate, urea, ammonium molybdate and calcium superphosphate are added and evenly mixed for granulation to obtain the fertilizer dedicated for tea trees. The method provides a new application for the tea old leaves, and also provides a new raw material which can extract the tea polyphenol and the theanine, and waste materials, i.e., the extracted tea old leaf dregs, can be made good use.

Description

technical field [0001] The invention relates to a method for extracting bioactive substances from old tea leaves, in particular to a method for extracting tea polyphenols and theanine from old tea leaves. Background technique [0002] Tea polyphenols and theanine are important active ingredients in tea. With the continuous development of the chemical synthesis methods of folic acid, tea polyphenols and theanine, more and more functions and uses of tea polyphenols and theanine It was discovered and proven, therefore, that polyphenols and theanine are in great demand. Most of the tea polyphenols and theanine used in the market are proposed from tea leaves, and the tea residues after extraction are thrown away, the cost of raw materials is high, and the source of raw materials is limited; therefore, tea polyphenols and theanine The chemical synthesis method of acid not only requires high production input for the production of tea polyphenols and theanine, but also needs to be ...

Claims

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Application Information

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IPC IPC(8): C07C237/06C07C231/24C07G99/00C05G1/00C05F17/00A23L33/105A23L33/175
CPCC05B1/02C07C231/24C07C237/06A23V2002/00C05F17/50C07G99/00A23V2250/2132A23V2250/21C05B7/00C05C3/00C05C9/00C05D1/02C05D9/02C05F3/00C05F5/006C05F11/08Y02W30/40
Inventor 马喆杜琼
Owner 湖北宇隆生物工程有限责任公司
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