Engineering bacteria of gynostemma pentaphylla glycosyl transferase, and construction method and applications thereof

A technology of glycosyltransferase and construction method, applied in the application field of the Gynostemma glycosyltransferase in synthesizing rare ginsenoside CK, can solve the problems of low yield, environmental pollution, complex ginsenoside process flow, etc. High, simple process, low consumption effect
CN106544310AActive Publication Date: 2017-03-29NANKAI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANKAI UNIV
Publication Date
2017-03-29

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Abstract

The invention discloses engineering bacteria of gynostemma pentaphylla glycosyl transferase, and a construction method and applications thereof. The gynostemma pentaphylla glycosyl transferase is capable of catalyzing protopanoxadiol to synthesize rare ginsenoside CK, and is named as UGTGp3. The invention also discloses a method used for synthesizing rare ginsenoside CK, and applications thereof. According to the method, heterologous expression of the glycosyl transferase gene of gynostemma pentaphylla is carried out in escherichia coli, and in vitro enzymatic reaction is adopted for functional identification. Relatively high signals are detected in ESI mass spectrometry and HPLC detection. The obtained rare ginsenoside CK is high in yield; less side product is generated; industrialized production is convenient to realize; and foundation is provided for gypenoside heterologous biosynthesis.
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Description

technical field

[0001] The invention belongs to the technical field of biopharmaceuticals, and relates to a method for synthesizing rare ginsenosides in vitro by expressing gypenosyltransferase heterologously in Escherichia coli, and also relates to the application of the gypenosyltransferase in synthesizing rare ginsenoside CK. Background technique

[0002] Gynostemma Gynostemma is a perennial herbaceous vine of Cucurbitaceae Gynostemma Gynostemma. Gynostemma Gynostemma contains a variety of medicinal ingredients, the most important of which is Gypenoside. The structure of Gypenoside is a tetracyclic triterpene dammarane type, which is the same as that of ginsenoside. Gypenoside is composed of two parts, which is a biomacromolecule formed by the combination of glycoside and glycoside. So far, it has been found that eight kinds of gypenosides are identical in structure to protopanaxadiol-type ginsenosides, and the total amount of these eight kinds of saponins reaches about ...

Claims

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