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A method for reducing the inhibition effect of by-products in lignocellulose alkaline pretreatment liquid and preparation of cellulosic ethanol based on this method

A technology of lignocellulose and pretreatment liquid, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of increasing process complexity, time-consuming, additional consumption of reagents and energy, and achieve the goal of promoting Industrial application, simple equipment requirements, and the effect of eliminating inhibition effects

Active Publication Date: 2021-03-16
GUANGDONG QIZHI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Saturated quicklime (over-liming) is a commonly used chemical detoxification method, which can remove most of the furfural in the lignocellulose hydrolyzate; but due to adsorption, it will cause about 10% loss of fermentable sugar
A disadvantage of most physical or chemical detoxification methods is that additional processing steps are required, which not only increases the complexity of the process, but also brings additional consumption of reagents and energy
The biological method uses microorganisms such as laccase, peroxidase or white rot fungus to oxidize and degrade the phenolic compounds in the hydrolyzate, and does not require new equipment and operations. However, the current biological method is time-consuming and harsh conditions affect its widely used
[0008] In this context, in order to improve the process of converting lignocellulose into ethanol, especially in view of the shortcomings of the current detoxification process, which are cumbersome, time-consuming, waste water, and energy-consuming, the present invention proposes a new type of biological detoxification of lignocellulose alkaline pretreatment liquid The method only needs to add one step of enzymatic hydrolysis reaction to complete detoxification and eliminate the inhibition of by-products on cellulase and yeast fermentation ability

Method used

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  • A method for reducing the inhibition effect of by-products in lignocellulose alkaline pretreatment liquid and preparation of cellulosic ethanol based on this method
  • A method for reducing the inhibition effect of by-products in lignocellulose alkaline pretreatment liquid and preparation of cellulosic ethanol based on this method
  • A method for reducing the inhibition effect of by-products in lignocellulose alkaline pretreatment liquid and preparation of cellulosic ethanol based on this method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] GOD treatment of bagasse pretreated with 1% NaOH and fermentation to produce ethanol

[0034] S1: Accurately weigh 2.5 g of bagasse, add 1% NaOH solution at a liquid-solid ratio of 10:1 (substrate concentration 10% (w / v)), treat bagasse at 121°C for 1 hour, and cool at room temperature to obtain a pre- treatment fluid;

[0035] S2: Under sterile conditions, add commercially available concentrated phosphoric acid to the pretreatment solution, adjust the pH to about 5, then add 10U GOD, detoxify at 30°C, 200rpm for 24h;

[0036] S3: Under sterile conditions, add cellulase to the above pretreatment solution, and fix the ratio of enzyme addition to substrate at 6 mg enzyme / g substrate, then place it in a constant temperature shaker, hydrolyze at 45 °C, 160 rpm for 72 hours; During the hydrolysis process, samples were taken to measure the enzymatic hydrolysis of bagasse;

[0037] S4: Then add peptone to a final concentration of 20g / L, and insert the pre-activated Saccharom...

Embodiment 2

[0040] GOD treatment of 2% NaOH pretreated bagasse and fermentation to produce ethanol

[0041] S1: Accurately weigh 2.5 g of bagasse, add 2% NaOH solution at a liquid-solid ratio of 10:1 (substrate concentration 10% (w / v)), treat bagasse at 121°C for 1 hour, and cool at room temperature to obtain a pre- treatment fluid;

[0042] S2: Under sterile conditions, add commercially available concentrated phosphoric acid to the pretreatment solution, adjust the pH to about 5, then add 15U GOD, detoxify at 30°C, 200rpm for 24h;

[0043] S3: Under sterile conditions, add cellulase to the above pretreatment solution, and fix the ratio of enzyme addition to substrate at 6 mg enzyme / g substrate, then place it in a constant temperature shaker, hydrolyze at 45 °C, 160 rpm for 72 hours; During the hydrolysis process, samples were taken to measure the enzymatic hydrolysis of bagasse;

[0044] S4: Then add peptone to a final concentration of 20g / L, and insert the pre-activated Saccharomyces ...

Embodiment 3

[0047] GOD treatment of 3% NaOH pretreated bagasse and fermentation to produce ethanol

[0048] S1: Accurately weigh 2.5 g of bagasse, add 3% NaOH solution at a liquid-solid ratio of 10:1 (substrate concentration 10% (w / v)), treat bagasse at 121°C for 1 hour, and cool at room temperature to obtain a pre- treatment fluid;

[0049] S2: Under sterile conditions, add commercially available concentrated phosphoric acid to the pretreatment solution to adjust the pH to about 5, then add 25U GOD, detoxify at 30°C, 200rpm for 24h;

[0050] S3: Under sterile conditions, add cellulase to the above pretreatment solution, and fix the ratio of enzyme addition to substrate at 6 mg enzyme / g substrate, then place it in a constant temperature shaker, hydrolyze at 45 °C, 160 rpm for 72 hours; During the hydrolysis process, samples were taken to measure the enzymatic hydrolysis of bagasse;

[0051] S4: Then add peptone to a final concentration of 20g / L, and insert the pre-activated Saccharomyce...

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Abstract

A method for reducing an inhibiting effect of byproducts in lignocellulose alkaline pretreatment liquid. The method is achieved by treating lignocellulose alkaline pretreatment liquid by means of glucose oxidase without changing a reaction container and comprises the following steps: S1, adding lignocellulose to alkali liquor for treatment to obtain pretreatment liquid; and S2, adding glucose oxidase to the pretreatment liquid obtained in step S2 for reaction.

Description

technical field [0001] The invention relates to the fields of biochemical engineering and fermentation engineering, in particular to a method for reducing the inhibition effect of by-products in lignocellulose alkaline pretreatment liquid and preparing ethanol based on the method. Background technique [0002] Lignocellulose has become an ideal raw material to replace fossil energy due to its wide source, rich content and renewable nature. The production of alternative energy sources such as bio-ethanol, bio-butanol, and bio-syngas from lignocellulose has become a research hotspot around the world. Since ethanol can be directly used in existing engines by mixing with gasoline in various proportions, and can be produced by microbial fermentation, the production of fuel ethanol from lignocellulose has become one of the focuses of research. [0003] Lignocellulose that can be used as a raw material for fuel ethanol production is mainly agricultural and forestry waste, such as ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12R1/865
CPCC12P7/10C12P2201/00Y02E50/10
Inventor 刘泽寰林蒋海黄清吕晓静傅菁李帅
Owner GUANGDONG QIZHI BIOTECHNOLOGY CO LTD
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