A kind of purification method of long-chain dicarboxylic acid in fermented liquid
A long-chain dicarboxylic acid and purification method technology, which is applied in the field of long-chain dicarboxylic acid purification, can solve the problems of low yield of dicarboxylic acid products, cumbersome operation steps, low recovery rate of alkanes, etc., to avoid production safety and environmental pollution, shortening the process flow, and high purity
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Embodiment 1
[0028] Using n-dodecane as substrate, use Candida tropicalis to ferment and produce dodecanedicarboxylic acid. At the end of the fermentation, the dicarboxylic acid concentration was 164.3 g / L and the pH was 7.4. Take 1000ml of fermentation broth, add 5% potassium dihydrogen phosphate to the fermentation broth, stir to dissolve the inorganic salt, continue stirring and then add 10% NaOH solution until the pH of the system is 10, and stir for 100 minutes. Stand still for 60min, reclaim the unreacted alkanes in the upper layer, and use a membrane with a pore size of 10 -2 Filter through a μm membrane to remove bacterial cells and debris. Add polyethylene glycol with a mass fraction of 5% and a molecular weight of 4000 to the system, stir to dissolve the polymer, and form an aqueous two-phase system. Send the system into the supergravity equipment for supergravity treatment, the supergravity level is 150g, the average residence time of the supergravity treatment is 5s, the syst...
Embodiment 2
[0030] Using n-dodecane as substrate, use Candida tropicalis to ferment and produce dodecanedicarboxylic acid. At the end of the fermentation, the dicarboxylic acid concentration was 158 g / L and the pH was 7.3. Take 1000ml of fermentation broth, add dipotassium hydrogen phosphate with a mass fraction of 10% to the fermentation broth, stir to dissolve the inorganic salt, continue stirring and then add NaOH solution with a mass fraction of 20%, until the system pH=12, stirring and mixing for 120min . Stand still for 80min, reclaim the unreacted alkanes in the upper layer, and use a membrane with a pore size of 10 -2 Filter through a μm membrane to remove bacterial cells and debris. Add polyethylene glycol with a mass fraction of 10% and a molecular weight of 10,000 to the system, stir to dissolve the polymer, and form an aqueous two-phase system. Send the system into the supergravity equipment for supergravity treatment, the supergravity level is 200g, the average residence t...
Embodiment 3
[0032]Using n-tridecane as substrate, use Candida tropicalis to ferment and produce tridecanedicarboxylic acid. At the end of the fermentation, the dicarboxylic acid concentration was 154.3 g / L and the pH was 7.5. Take 1000ml of fermentation broth, add ammonium sulfate with a mass fraction of 15% to the fermentation broth, stir to dissolve the inorganic salt, continue stirring and then add NaOH solution with a mass fraction of 30%, until the system pH=12, and stir for 20 minutes. Stand still for 100min, reclaim the unreacted alkanes in the upper layer, and use a membrane with a pore size of 10 -2 Filter through a μm membrane to remove bacterial cells and debris. Add polyethylene glycol with a mass fraction of 12% and a molecular weight of 20,000 to the system, and stir to dissolve the polymer to form a two-phase aqueous system. Send the system into the supergravity equipment for processing, the supergravity level is 300g, the average residence time of the supergravity treatm...
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