Electrostatic layer-by-layer self assembling technology modified bioengineering porcine cornea and production method thereof

A layer-by-layer self-assembly and bioengineering technology, applied in the medical field, can solve the problems of limiting the application of corneal transplantation, large variability of vision restoration, and large destructiveness of corneal tissue, so as to enhance the ability to resist pathological environments and promote visual function. Restoration, light transmittance ideal effect

Active Publication Date: 2017-04-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the lack of donor tissue, graft rejection, failure, and large variability in visual recovery have limited the application of corneal transplantation to a certain extent.
[0005] At present, there are many methods for the preparation of bioengineered corneas, including Triton-X100 (polyethylene glycol octylphenyl ether), EDTA (ethylenediaminetetraacetic acid) , SDS (sodium dodecylsulfonate), trypsin, DNA / RNase, although they can obtain decellularized corneal scaffolds with low immunogenicity and low cell residues, they react violently, are destructive to corneal tissue, and face protein denaturation , GAG (glycosaminoglycan) content reduction and other issues

Method used

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  • Electrostatic layer-by-layer self assembling technology modified bioengineering porcine cornea and production method thereof
  • Electrostatic layer-by-layer self assembling technology modified bioengineering porcine cornea and production method thereof
  • Electrostatic layer-by-layer self assembling technology modified bioengineering porcine cornea and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The bioengineered porcine cornea modified by electrostatic layer-by-layer self-assembly technology, the specific preparation process is as follows:

[0033] 1) Clean the bioengineered porcine cornea with a bicarbonate mixed solution, and then dry it for 8 minutes at a temperature of 30 degrees Celsius in an ultra-clean bench;

[0034]2) Immerse the dried bioengineered porcine cornea in a positively charged chitosan aqueous solution with a concentration of 1 mg / ml and pH 3.0, absorb for 5 minutes, and rinse with deionized water; then immerse the bioengineered porcine cornea in a negatively charged concentration of 10mg / ml, pH6.0 sodium hyaluronate solution, adsorption for 30 minutes, rinsed with deionized water; In the chemical factor solution, absorb for 30 minutes, and then rinse with deionized water;

[0035] 3) Repeat step 2) 20 times, so that the polycation, hyaluronic acid layer and cell chemokine layer are alternately adsorbed on the surface of the bioengineered ...

Embodiment 2

[0038] The bioengineered porcine cornea modified by electrostatic layer-by-layer self-assembly technology, the specific preparation process is as follows:

[0039] 1) Clean the bioengineered porcine cornea with a bicarbonate mixed solution, and then dry it for 10 minutes at a temperature of 20 degrees Celsius in an ultra-clean bench;

[0040] 2) Immerse the dried bioengineered porcine cornea into a positively charged polylysine aqueous solution with a concentration of 10 mg / ml and pH6.0, absorb for 30 minutes, and rinse with deionized water; In the sodium hyaluronate solution with a charge concentration of 1 mg / ml and a pH of 3.0, absorb for 5 minutes and rinse with deionized water; In the chemokine solution, adsorb for 5 minutes, and then rinse with deionized water;

[0041] 3) Repeat step 2) 10 times, so that the polycation, hyaluronic acid layer and cell chemokine layer are alternately adsorbed on the surface of the bioengineered pig cornea, and ensure that the outermost l...

Embodiment 3

[0044] The bioengineered porcine cornea modified by electrostatic layer-by-layer self-assembly technology, the specific preparation process is as follows:

[0045] 1) Clean the bioengineered porcine cornea with a bicarbonate mixed solution, and then dry it for 12 minutes at a temperature of 10 degrees Celsius in an ultra-clean bench;

[0046] 2) Immerse the dried bioengineered porcine cornea in a positively charged chitosan aqueous solution with a concentration of 6 mg / ml and pH 4.5, absorb for 18 minutes, and rinse with deionized water; then immerse the bioengineered porcine cornea in a negatively charged solution. The concentration of 6mg / ml, pH4.5 sodium hyaluronate solution, adsorption for 18 minutes, rinsed with deionized water; In the chemical factor solution, absorb for 20 minutes, and then rinse with deionized water;

[0047] 3) Repeat step 2) 15 times, so that the polycation, hyaluronic acid layer and cell chemokine layer are alternately adsorbed on the surface of th...

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Abstract

The invention relates to an electrostatic layer-by-layer self assembling technology modified bioengineering porcine cornea and a production method thereof. The bioengineering porcine cornea provided by the invention promotes limbal stem cells and cornea epithelial cells to migrate after cornea transplantation, and the production method is simple, and is easy to implement. Polycationic layers, hyaluronic acid layers and cell chemotactic factor layers are sequentially repeatedly deposited on the surface of a bioengineering porcine cornea, and the cell chemotactic factor layer is the outermost layer. The production method sequentially comprises the following steps: 1, cleaning and drying the bioengineering porcine cornea; 2, sequentially forming the polycationic layers, the hyaluronic acid layers and the cell chemotactic factor layers on the surface of the porcine cornea; 3, repeating step 2 to make the polycationic layers, the hyaluronic acid layers and the cell chemotactic factor layers be continuously formed on the surface of the porcine cornea from inside to outside, and guaranteeing that the cell chemotactic factor layer is the outermost layer; and 4, drying the modified bioengineering porcine cornea, and carrying out sealed packaging to obtain the electrostatic layer-by-layer self assembling technology modified bioengineering porcine cornea.

Description

technical field [0001] The invention relates to the medical field, in particular to bioengineering porcine cornea modified by electrostatic layer-by-layer self-assembly technology and a preparation method thereof. Background technique [0002] The cornea is a layer of transparent tissue on the front wall of the eyeball that mainly acts as a refractive and barrier function. A variety of factors such as degenerative changes, malnutrition, infection, inflammation, injury, involvement of other diseases in the eye, etc. can lead to corneal disease and even blindness. Corneal disease is currently the second leading cause of blindness after cataracts. Allogeneic corneal transplantation, that is, human corneal transplantation, is still the first choice for the treatment of blindness due to corneal diseases. The current surgical methods include penetrating keratoplasty, lamellar keratoplasty, endothelial keratoplasty and so on. However, the lack of donor tissue, graft rejection, f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/22A61L27/20A61L27/18A61L27/54
CPCA61L27/18A61L27/20A61L27/22A61L27/54A61L2300/252A61L2430/16C08L5/08C08L77/04
Inventor 姚克王本方芝阮韦淑怡贺川江
Owner ZHEJIANG UNIV
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