Preparation method and application of MoS2/Au-Pd compound-based procalcitonin electrochemical immunosensor

An immunosensor, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of unsuitable rapid detection, large sample consumption, complex pretreatment, etc., achieve low detection limit, simplify preparation process, the effect of good catalytic effect

Inactive Publication Date: 2017-04-19
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods have high sensitivity and selectivity, but the detection process requires expensive special instrument...

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  • Preparation method and application of MoS2/Au-Pd compound-based procalcitonin electrochemical immunosensor
  • Preparation method and application of MoS2/Au-Pd compound-based procalcitonin electrochemical immunosensor

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Experimental program
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Embodiment 1

[0031] Embodiment 1. A MoS-based 2 The preparation method of the electrochemical immunosensor of Au-Pd composite:

[0032] (1) Use 1.0 μm, 0.3 μm, 0.05 μm Al 2 o 3 The glassy carbon electrode (GCE) with a diameter of 4 mm was polished successively with polishing powder, ultrasonically cleaned in ethanol and ultrapure water for 3 min, dried with nitrogen, and 6 μL MoS 2 / Au-Pd nanocomposite dispersion liquid was drop-coated on the electrode surface, dried at room temperature to form a film, and washed with PBS (pH 7.4) buffer solution to remove unbonded MoS 2 / Au-Pd to get Au-Pd / MoS 2 / GCE;

[0033] (2) Take 10 μL of 6 μg / mL procalcitonin antibody (anti-PCT) standard solution and apply it dropwise on the surface of the electrode, incubate overnight at 4 °C, wash with PBS (pH 7.4) buffer solution to obtain anti-PCT / Pd -Au / MoS 2 / GCE;

[0034] (3) Take 10 μL bovine serum albumin (BSA) solution with a mass fraction of 1% drop-coated on the surface of the electrode, incubate...

Embodiment 2

[0036] Example 2. A MoS-based 2 The preparation method of the electrochemical immunosensor of Au-Pd composite:

[0037] (1) Use 1.0 μm, 0.3 μm, 0.05 μm Al 2 o 3 The glassy carbon electrode (GCE) with a diameter of 4 mm was polished sequentially with polishing powder, ultrasonically cleaned in ethanol and ultrapure water for 3 min, and dried with nitrogen, and 8 μL MoS 2 / Au-Pd nanocomposite dispersion liquid was drop-coated on the electrode surface, dried at room temperature to form a film, and washed with PBS (pH 7.4) buffer solution to remove unbonded MoS 2 / Au-Pd to get Au-Pd / MoS 2 / GCE;

[0038] (2) Take 10 μL of 8 μg / mL procalcitonin antibody (anti-PCT) standard solution and apply it dropwise on the surface of the electrode, incubate overnight at 4 °C, wash with PBS (pH 7.4) buffer solution to obtain anti-PCT / Pd -Au / MoS 2 / GCE;

[0039] (3) Take 10 μL of bovine serum albumin (BSA) solution with a mass fraction of 2% and drop-coat it on the surface of the electrode,...

Embodiment 3

[0041] Example 3. A MoS-based 2 The preparation method of the electrochemical immunosensor of Au-Pd composite:

[0042] (1) Use 1.0 μm, 0.3 μm, 0.05 μm Al 2 o 3 The glassy carbon electrode (GCE) with a diameter of 4 mm was polished successively with polishing powder, ultrasonically cleaned in ethanol and ultrapure water for 3 min, and dried with nitrogen, and 10 μL MoS 2 / Au-Pd nanocomposite dispersion liquid was drop-coated on the electrode surface, dried at room temperature to form a film, and washed with PBS (pH 7.4) buffer solution to remove unbonded MoS 2 / Au-Pd to get Au-Pd / MoS 2 / GCE;

[0043] (2) Take 10 μL of 10 μg / mL procalcitonin antibody (anti-PCT) standard solution and apply it dropwise on the surface of the electrode, incubate overnight at 4 °C, wash with PBS (pH 7.4) buffer solution to obtain anti-PCT / Pd -Au / MoS 2 / GCE;

[0044] (3) Take 10 μL of bovine serum albumin (BSA) solution with a mass fraction of 3% and drop-coat it on the surface of the electrod...

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Abstract

The invention relates to the field of electrochemical immunosensing technique, especially to a preparation method and application of a MoS2/Au-Pd compound-based label-free electrochemical immunosensor. H2O2 is used as an electrochemical probe, and a MoS2/Au-Pd compound is used as a substrate material. Based on good film forming capability and large specific surface area and excellent catalytic performance to H2O2, stability and sensitivity of an immunosensor are remarkably enhanced. The testing principle is to utilize current change before and after an antigen-antibody immunoreaction. When an antigen reacts with an antibody modified on an electrode, the formed immunocomplex is a non-electroactive substance and severely obstructs electron transfer, and current signal is reduced. The sensor is used for detection of procalcitonin. Linearity range for detection is 0.0001 ng/mL- 10 ng/mL, and detection limit is 0.05 pg/mL.

Description

technical field [0001] The invention relates to the technical field of electrochemical immunosensing, in particular to a MoS-based 2 Preparation and application of procalcitonin (PCT) electrochemical immunosensor of / Au-Pd substance. Specifically related to a H 2 o 2 As an electrochemical probe, MoS 2 Construction and application of electrochemical immunosensors based on / Au-Pd composites. Background technique [0002] PCT belongs to a kind of calcitonin propeptide substance, which has no hormone activity, and its elevated level in serum is caused by severe infection and destruction of bacteria, fungi, parasites, sepsis and multiple organ failure; in itself The content will not increase during immunity, allergy and virus infection. Therefore, PCT can diagnose systemic infections caused by bacteria and fungi, and is regarded as a routine index for auxiliary and differential diagnosis of systemic bacterial infections and sepsis. At present, the detection methods for PCT ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N27/327G01N27/30
CPCG01N33/68G01N27/308G01N27/3278G01N2333/585
Inventor 花小霞周长利王月姣
Owner UNIV OF JINAN
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