Preparation method of fluorescent sensor system based on cationic conjugated polymer and iridium complex FRET effect and application thereof

A conjugated polymer, fluorescence sensor technology, used in chemical instruments and methods, fluorescence/phosphorescence, indium organic compounds, etc.

Active Publication Date: 2017-04-26
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no relevant reports on the fluorescent sensor system and its application b

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  • Preparation method of fluorescent sensor system based on cationic conjugated polymer and iridium complex FRET effect and application thereof
  • Preparation method of fluorescent sensor system based on cationic conjugated polymer and iridium complex FRET effect and application thereof
  • Preparation method of fluorescent sensor system based on cationic conjugated polymer and iridium complex FRET effect and application thereof

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Embodiment 1

[0045] A method for preparing a fluorescent sensor system based on the FRET effect of cationic conjugated polymers and iridium complexes, the specific steps are as follows:

[0046] (1) Synthesis of iridium complexes

[0047]A. Add 10-15mmol 2-chloropyrimidine, 10-20mmol 2,4-difluorophenylboronic acid, 1.25-1.5mmol triphenylphosphine, 10-15mL ethylene glycol dimethyl ether and 15-20mL potassium carbonate solution into the three-necked flask, and pass nitrogen gas at room temperature for 30-60 minutes, then add 0.3-0.5 mmol of palladium acetate into the three-necked flask, stir magnetically, and reflux at 110-150°C for 15-20 hours to stop the reaction, cool to room temperature, and divide Extract the organic phase and the water phase; extract the water phase with 30-60 mL of ethyl acetate, then repeat the extraction of the water phase with 30-60 mL of ethyl acetate three times; combine the organic phases with 40-80 mL of water and 40-80 mL of brine in sequence Wash, then dry w...

Embodiment 2

[0071] The fluorescent sensor system based on the FRET effect of cationic conjugated polymers and iridium complexes detects adenosine triphosphate. The specific method steps are as follows: add 2 μL of 10 μM adenosine triphosphate aptamer solution to 100 μL of the cationic conjugated polymer prepared in the above specific example 1. In the fluorescent sensor system with the FRET effect of the iridium complex, then add different concentrations of adenosine triphosphate solutions to the fluorescent sensor system, then add the buffer solution, incubate at 37°C for 40-60min, detect the change of fluorescence intensity, and calculate the positive ion The fluorescence intensity ratio of the conjugated polymer (415nm) and the iridium complex (530nm) was obtained to obtain a series of fluorescence intensity ratios corresponding to different concentrations of adenosine triphosphate, and the quantitative relationship between the fluorescence intensity ratio and the concentration of adenos...

Embodiment 3

[0074] The fluorescent sensor system based on the FRET effect of cationic conjugated polymers and iridium complexes detects platelet-derived growth factor, and the specific method steps are as follows: add 2 μL of 10 μM platelet-derived growth factor aptamer solution to 100 μL of the aptamer prepared in the above specific example 1 In the fluorescent sensor system based on the FRET effect of cationic conjugated polymers and iridium complexes, then add different concentrations of platelet-derived growth factor solutions into the fluorescent sensor system, then add buffer solution, and incubate at 37°C for 40-60min , detect changes in fluorescence intensity, calculate the fluorescence intensity ratio of the cationic conjugated polymer (415nm) to the fluorescence intensity of the iridium complex (530nm), obtain a series of fluorescence intensity ratios corresponding to different concentrations of platelet-derived growth factor, and establish the fluorescence intensity The quantita...

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Abstract

The invention discloses a preparation method of fluorescent sensor system based on cationic conjugated polymer and iridium complex FRET effect and application thereof and is characterized in that it comprises the synthesis step of the iridium complex; the synthesis step of the cationic conjugated polymer;the cationic conjugated polymer and iridium complexes give rise to FRET and obtain the fluorescence sensor system steps based on the FRET effect of the cationic conjugated polymer and iridium complex; the fluorescence sensor system can be used to detect thrombin, adenosine triphosphate, platelet derived growth factor, cocaine and lysozyme . The invention has the advantages of good specificity, high sensitivity, accurate and reliable results, low cost, high speed and simple process.

Description

technical field [0001] The invention relates to a fluorescent sensor system, in particular to a preparation method and application of a fluorescent sensor system based on the FRET effect of cationic conjugated polymer and iridium complex. Background technique [0002] Fluorescence resonance energy transfer (FRET) is a widely used principle for designing fluorescence sensors. Fluorescence resonance energy transfer means that when two fluorescent chromophores are close enough, when the donor molecule absorbs photons of a certain frequency, it is excited to a higher electronic energy state, and before the electron returns to the ground state, it passes through the dipole The interaction realizes the transfer of energy to the adjacent acceptor molecules, that is, energy resonance transfer occurs. The conditions for effective energy transfer between the energy donor and the acceptor are harsh, mainly including: (1) the emission spectrum of the energy donor must overlap with the ...

Claims

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Application Information

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IPC IPC(8): C08G61/02C07F15/00G01N21/64
CPCC07F15/004C08G61/02C08G2261/18C08G2261/3142G01N21/6486
Inventor 胡宇芳杜春暖葛国平郭智勇王邃张青青
Owner NINGBO UNIV
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