Preparation method of non-toxic degradable transpiration inhibiter

A technology of transpiration inhibitors and preparation steps, which is applied in the field of preparation of non-toxic and degradable transpiration inhibitors, which can solve the problems of persistent pollution, difficulty in natural degradation, toxicity, etc., and achieve the effect of broad application prospects

Inactive Publication Date: 2017-05-10
孙祎
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: Aiming at the problem that traditional transpiration inhibitors are toxic, harmful to the environment and human health, difficult to degrade naturally, and have persistent pollution, a heat-resistant Bacillus coagulans enriched by soybean meal is provided, Milk and soybeans are fermented at medium temperature, and the fat protein complex cooked in milk and soybeans is loaded onto the surface of cellulose film produced by bacteria, which is ground and sprayed on the surface of leaves, and forms a layer after drying Composite film, a method for effectively closing the stomata on the surface of leaves and plants, and inhibiting the transpiration of water vapor on the surface of plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] First weigh 15g of soybean meal, 700mL of deionized water, 10g of sodium chloride, 15g of dipotassium hydrogen phosphate, and 2g of potassium manganate and place them in a petri dish, stir and mix them and sterilize them at 125°C for 15 minutes, then let them cool down After reaching room temperature, prepare a sterilized enrichment medium; use an inoculation loop to select 3 loops of Bacillus coagulans to transfer to the above sterilized enrichment medium, control the culture temperature to 50°C, and culture on a shaking table at 200r / min 8 days, after the cultivation is completed, filter with three layers of gauze and collect the filtrate to prepare the enriched strain liquid; then weigh 50 parts of fresh milk, 15 parts of soybeans, 10 parts of sodium chloride, and 15 parts of dipotassium hydrogen phosphate, 2 parts of potassium manganate and 50 parts of deionized water were placed in a beaker, stirred and mixed, heated in a water bath at 110°C for 30 minutes, allowed ...

example 2

[0020] First, weigh 10g of soybean meal, 650mL of deionized water, 5g of sodium chloride, 10g of dipotassium hydrogen phosphate, and 1g of potassium manganate and place them in a petri dish, stir and mix them and sterilize them at 120°C for 10 minutes, then let them stand to cool After reaching room temperature, prepare a sterilized enrichment medium; use an inoculation loop to select 2 loops of Bacillus coagulans to transfer to the above sterilized enrichment medium, control the culture temperature to 45°C, and culture on a shaking table at 120r / min After 5 days, after the cultivation is completed, filter with three layers of gauze and collect the filtrate to prepare the enriched strain liquid; then weigh 45 parts of fresh milk, 10 parts of soybeans, 5 parts of sodium chloride, and Put 10 parts of dipotassium hydrogen phosphate, 1 part of potassium manganate and 45 parts of deionized water in a beaker, stir and mix them, heat them in a water bath at 100°C for 25 minutes, and l...

example 3

[0023] First weigh 12g of soybean meal, 670mL of deionized water, 7g of sodium chloride, 12g of dipotassium hydrogen phosphate, and 2g of potassium manganate and place them in a petri dish, stir and mix them and place them in a high-temperature sterilization process at 122°C for 12 minutes, then let them stand to cool After reaching room temperature, prepare a sterilized enrichment medium; use an inoculation loop to select 2 loops of Bacillus coagulans to transfer to the above sterilized enrichment medium, control the culture temperature at 47°C, and culture on a shaking table at 130r / min After 7 days, after the cultivation is completed, filter with three layers of gauze and collect the filtrate to prepare the enriched strain liquid; then weigh 47 parts of fresh milk, 12 parts of soybeans, 7 parts of sodium chloride, and 12 parts of dipotassium hydrogen phosphate, 2 parts of potassium manganate and 47 parts of deionized water were placed in a beaker, stirred and mixed, heated i...

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Abstract

The invention relates to a preparation method of a non-toxic degradable transpiration inhibiter, and belongs to the technical field of a transpiration inhibiter. According to the method, substances such as soybean meal and sodium chloride are used as raw materials; a sterilization enrichment culture medium is prepared; bacillus coagulans are inoculated onto the sterilization enrichment culture medium for culture; through filtration, enriched strain liquid is obtained; then, the enriched strain liquid is inoculated onto a self-made sterilization culture medium; heating is performed; culture medium surface layer films are collected; drying is performed; grinding and sieving are performed to obtain fermentation film powder; finally, the fermentation film powder is subjected to homogenizing treatment with substances such as deionized water and monopotassium phosphate; the non-toxic degradable transpiration inhibiter can be obtained. The non-toxic degradable transpiration inhibiter prepared by the preparation method has the advantages that the plant transpiration speed can be effectively reduced; no toxic substances are contained; the harm to the environment and the human body health cannot be caused; the degradation is easy; the durable pollution cannot be caused; wide application prospects are realized.

Description

technical field [0001] The invention relates to a method for preparing a non-toxic and degradable transpiration inhibitor, belonging to the technical field of transpiration inhibitor preparation. Background technique [0002] Transpiration inhibitor is a kind of medicament that reduces water evaporation, protects plants and improves survival. Its mechanism of action is to form a protective film on the surface of leaves by spraying evenly on leaves and branches of plants, closing stomata and slowing down metabolism. The unique mesh structure can ensure the normal respiration and reduce water evaporation, thereby improving the survival rate of trees. Generally, it is used before and after the transplantation of large trees and during transportation, high temperature or drought, large-scale seedlings in landscaping projects, ancient trees, rare trees, etc., and evergreen trees and shrubs transplanted. [0003] The existing transpiration inhibitors mainly include: ① Metabolic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00
CPCC05B7/00C05G3/00C05D1/00C05D9/00C05F1/007C05F5/002C05F11/00C05F11/08
Inventor 孙祎薛红梅韩彪
Owner 孙祎
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