Preparation method of non-toxic degradable transpiration inhibiter
A technology of transpiration inhibitors and preparation steps, which is applied in the field of preparation of non-toxic and degradable transpiration inhibitors, which can solve the problems of persistent pollution, difficulty in natural degradation, toxicity, etc., and achieve the effect of broad application prospects
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example 1
[0017] First weigh 15g of soybean meal, 700mL of deionized water, 10g of sodium chloride, 15g of dipotassium hydrogen phosphate, and 2g of potassium manganate and place them in a petri dish, stir and mix them and sterilize them at 125°C for 15 minutes, then let them cool down After reaching room temperature, prepare a sterilized enrichment medium; use an inoculation loop to select 3 loops of Bacillus coagulans to transfer to the above sterilized enrichment medium, control the culture temperature to 50°C, and culture on a shaking table at 200r / min 8 days, after the cultivation is completed, filter with three layers of gauze and collect the filtrate to prepare the enriched strain liquid; then weigh 50 parts of fresh milk, 15 parts of soybeans, 10 parts of sodium chloride, and 15 parts of dipotassium hydrogen phosphate, 2 parts of potassium manganate and 50 parts of deionized water were placed in a beaker, stirred and mixed, heated in a water bath at 110°C for 30 minutes, allowed ...
example 2
[0020] First, weigh 10g of soybean meal, 650mL of deionized water, 5g of sodium chloride, 10g of dipotassium hydrogen phosphate, and 1g of potassium manganate and place them in a petri dish, stir and mix them and sterilize them at 120°C for 10 minutes, then let them stand to cool After reaching room temperature, prepare a sterilized enrichment medium; use an inoculation loop to select 2 loops of Bacillus coagulans to transfer to the above sterilized enrichment medium, control the culture temperature to 45°C, and culture on a shaking table at 120r / min After 5 days, after the cultivation is completed, filter with three layers of gauze and collect the filtrate to prepare the enriched strain liquid; then weigh 45 parts of fresh milk, 10 parts of soybeans, 5 parts of sodium chloride, and Put 10 parts of dipotassium hydrogen phosphate, 1 part of potassium manganate and 45 parts of deionized water in a beaker, stir and mix them, heat them in a water bath at 100°C for 25 minutes, and l...
example 3
[0023] First weigh 12g of soybean meal, 670mL of deionized water, 7g of sodium chloride, 12g of dipotassium hydrogen phosphate, and 2g of potassium manganate and place them in a petri dish, stir and mix them and place them in a high-temperature sterilization process at 122°C for 12 minutes, then let them stand to cool After reaching room temperature, prepare a sterilized enrichment medium; use an inoculation loop to select 2 loops of Bacillus coagulans to transfer to the above sterilized enrichment medium, control the culture temperature at 47°C, and culture on a shaking table at 130r / min After 7 days, after the cultivation is completed, filter with three layers of gauze and collect the filtrate to prepare the enriched strain liquid; then weigh 47 parts of fresh milk, 12 parts of soybeans, 7 parts of sodium chloride, and 12 parts of dipotassium hydrogen phosphate, 2 parts of potassium manganate and 47 parts of deionized water were placed in a beaker, stirred and mixed, heated i...
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