A recombinant Torulopsis glabrata co-producing pyruvate and α-ketoglutarate

Abstract

The invention discloses a recombinant torulopsis glabrata capable of co-producing pyruvic acid and alpha-ketoglutaric acid, and belongs to the technical field of fermentation engineering. Through a gene engineering technique, CDC19 originated from brewer's yeast is integrated into a pY26-TEF-GPD expression vector to establish brewer's yeast recombinant expression plasmid pY26-CDC19, and then the recombinant expression plasmid pY26-CDC19 is electrotransformed into a recipient bacterium TgU- to obtain an engineering strain TgU-(pY26-CDC19) with a high yield of pyruvic acid. Compared with control bacteria, the total acid yield (pyruvic acid and alpha-ketoglutaric acid), the total acid conversion rate, and the total acid production intensity are improved by 112.2%, 115.6%, and 155.6%. Most importantly, the output of alpha-ketoglutaric acid of the recombinant bacterium TgU-(pY26-CDC19) is increased from 0 g / L to 30.1 g / L after modification.

Description

Technical field

[0001] The invention relates to a recombinant S. glabrata for co-producing pyruvate and α-ketoglutarate, and belongs to the technical field of fermentation engineering. Background technique

[0002] Keto acids are compounds containing both carboxyl and keto groups in the molecule. According to the relative positions of the carboxyl and ketone groups in the molecule, they can be divided into α and β-keto acids. They are a kind of organic acids that play an important role in the living body. At the same time, the demand for them in the society is increasing. They are widely used Daily chemical, food, medical, agriculture and other industries. Among them, α keto acid is a keto acid with a carboxyl group on the α carbon atom, and pyruvate is the simplest α keto acid. As an intermediate product of biological metabolism, pyruvate is in the middle of various metabolic pathways and is the final product of glycolysis; it is converted into acetyl-CoA, the starting materia...

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